Evaluation of HumDNA Typing (Yanhuang-PCR) Kit for Usability and Reliability in Forensic Sciences and Comparison with Two Different PCR Kits (Identifiler Plus & GlobalFiler
Öz
Forensic science is a multidisciplinary field in which experts in
different fields collaborate to shed light on crime. Forensic genetics, a discipline to distinguish crime commuters/innocents, has
a great place within the scope of forensic sciences. Here, Short
Tandem Repeat (STR) analyses are the most frequently conducted forensic genetic analyses applied in interval situations such as
kinship determinations, parenting tests, comparison of evidence
obtained from the crime scene and/or suspects, or identification
of individuals. The number of these biomarkers varies in the content of the kits benefited in the analysis. To support the aim of
the study, the usability of the HUMDNA Typing Yanhuang PCR
Kit in the field of forensic sciences is evaluated. In this study, in
addition to two different kits—Identifiler Plus and GlobalFiler—
frequently preferred in the field, the HUMDNA Typing Yanhuang
PCR Kit is analyzed, and all three types of kits are compared. In
terms of discrimination power in forensic sciences, the variety of
number of examined loci is vitally important. In addition to this
comparison, three different size standards (LIZ500, LIZ600, and
Salmon 500 included in the kit) are tested in the electrophoresis of
samples amplified—using the HUMDNA Typing Yanhuang PCR
Kit—and cleaner results are obtained with LIZ600. Besides, PCR
mixtures at different ratios (1, 1/2.5, 1/5) are prepared and tested
to demonstrate how helpful the kit works in eliminating insufficient sample deficiencies—a crucial issue in forensic sciences.
To obtain bear fruits in conducted studies in every ratio, results
should be achieved by minute quantity of DNA. Thereby, the
HUMDNA Typing Yanhuang PCR Kit is supposed a beneficial
kit for genetic studies carried out within forensic sciences. This
study suggests that essential data—as well as possible population
studies—are potentially added to the literature to be conducted in
the following studies.
Anahtar Kelimeler
Yanhuang PCR Kit, HumDNA Typing Kit, Identifiler Plus, GlobalFiler, Short Tandem Repeats (STRs), Forensic Genetics
Kaynakça
- 1. Li C. Forensic genetics. Forensic Sciences Research. 2018;3(2):103-104. https:// doi.org/10.1080/20961790.2018.1489445
- 2. Wyner N, Barash M, McNevin D. Forensic autosomal short tandem repeats and their potential association with phenotype. Frontiers in genetics. 2020; 11: 884. https://doi.org/10.3389/fgene.2020.00884
- 3. Butler JM. Short tandem repeat typing Technologies are used in human identity testing. BioTechniques 2018; 43(4). https://doi.org/10.2144/000112582
- 4. Validation Information to Aid Forensic DNA Laboratories [Internet]. National Institute of Standards and Technology (NIST) STRBase; [cited 2023 April 15] Available from: https://strbase-archive.nist.gov/validation.htm
- 5. Core STR Loci Used in Human Identity Testing [Internet]. National Institute of Standards and Technology (NIST) STRBase; [cited 2023 Jan 09] Available from: https://strbase-archive.nist.gov/coreSTRs.htm
- 6. https://assets.thermofisher.com/TFS-Assets/LSG/manuals/4440211_AmpFlSTR_IdentifilerPlus_UG.pdf (Son Erişim Tarihi 3 Mart 2023).
- 7. https://assets.thermofisher.com/TFS-Assets/LSG/manuals/4477604.pdf (Son Erişim Tarihi 3 Mart 2023).
- 8. https://e.fgidna.com/tests?id=31 (Son Erişim Tarihi 4 Nisan 2023).
- 9. Fung WK, Hu Y. Statistical DNA forensics: theory, methods and computation. 1st ed. John Wiley & Sons; 2008. ISBN: 978-0-470-06636-2
- 10. Dash HR, Das S. Microbial degradation of forensic samples of biological origin: a potential threat to human DNA typing. Molecular biotechnology 2018; 60(2): 141-153. https://doi.org/10.1007/s12033-017-0052-5