Türkiye'de Kültür Şartlarında Büyütülen Rus Mersin Balıklarından (Acipenser gueldenstaedtii) Sperm Alınması ve Muhafazası

Öz

Bu çalışmada bütün dünyada nesilleri azalan Asipenceridae familyasının Karadeniz ve ona bağlı nehirlerde yaşayan Rus mersininden kültür şartlarında sperm alınması ve cryopreservasyonu çalışılmıştır. Semen abdominal masaj ile toplanmıştır. Çalışmada spermlerin zamana bağlı hız ve hareketleri 5 kategoride değerlendirilmiştir. Soğutma 0 ile -4°C arasında  3°C/dakika ve -4 ile -10 °C arasında  5°C uygulanmıştır. Taze semenler %8 DMSO Karyoprotectantı kullanılarak 5 farklı ekstendır ile -196 °C’de sıvı azot içinde dondurulmuştur. Spermatokrite oranları %65 ile %91 arasında değişen spermlerin pH’sı ortalama 8.0 olmuştur. Dondurmadan 24 saat sonra sperm örnekleri 32°C su içinde 2 dakika düzenli çalkalanarak çözülmüş ve spermleri harektlendirmek için su ilave edilmiştir. Taze spermlerin su ile aktivasyonundan 10 dakika sonra hızları ve hareketlilikleri sırasıyla ++ ve %20 olarak belirlenmiştir. Elde ettiğimiz sonuçlar E2 ekstenderinin diğerlerinden daha iyi olduğunu göstermiş ve Mersin balıklarının semeninin soğuk muhafazasında faydalı bir ekstendir olabilir.

Anahtar Kelimeler

• Karaca mersini,muhafaza

Semen Collection and Cryopreservation of Russian Sturgeon (Acipenser gueldenstaedtii) Reared in Turkey

Öz

In this research, milt collection and cryopreservation with different extenders in endangered Russian sturgeon (Acipencer gueldenstaedtii) which lives Black Sea and its tributary rivers  were studied. The semen was collected by abdominal massage. Sperm motility classified to five categories. Cooling were carried out as follow; 3°C/min between 0 and -4 and 5°C/min between -4 and -10. Sperm were frozen using 5 different extenders and with 8% DMSO as cyroprotectant at -196 °C in liquid nitrogen. Spermatocrite ratios range from 65% to 91%. Sperm pH was determined 8.0. After 24 hours the deep frozen sperm samples were thawed by quickly immersing them in a water bath at 32 ºC under constant shaking for about 2 min, and freshwater was added to stimulate the sperm motility. In ten minutes after activation, the velocity and motility of sperm were ++ and 20%. Our results indicate that E2 extender was better than the others and may useful extender for cryopreservation of sturgeon semen

Anahtar Kelimeler

• Sturgeon,sperm,cryopreservation,motility

Kaynakça

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