Temperature response to sperm motility of Russian sturgeon semen during the post-activation period

Öz

In this study, sperm quality of Russian sturgeon (Acipenser gueldenstaedtii Brandt, 1883) and effect of temperature on sperm motility and viability after diluting with fresh water studied. The sperm were collected from four males after 0.5 µg/kg surfagone injection. After sperm quality determined, diluted with fresh water and kept various temperatures from 4 to 24C°. The spermatozoa motility and viability were examined five minutes intervals until motionless stage. The motility and viability of Russian sturgeon spermatozoa decreases when the temperature is increased from 4 to 24°C. Motility was seen to in progress at the 14°C which is close to incubation degree of Russian sturgeon eggs until 28^th minute and viability recorded as 5% percent at that time. The viability of spermatozoa was determined more than 50 percent at the 14°C, therefore Russian sturgeon sperm can be use fertilization until 8 min after activated with fresh water.

Anahtar Kelimeler

• Sperm motility,Russian sturgeon,temperature,reproduction

Sıcaklığın Karaca Mersini Spermlerinin Sulandırmadan Sonraki Hareketliliğine Etkisi

Öz

Bu çalışmada karaca mersininin (Acipenser gueldenstaedtii Brandt, 1883) sperm kalitesi ve sulandırmadan sonra sıcaklığın sperm hareketliliği ve yaşama süresine etkisi çalışılmıştır. Spermler, 0.5 µg/kg sulfagon enjeksiyonu yapılan dört balıktan alınmıştır. Sperm kalitesi belirlendikten sonra, tatlı su ile sulandırılarak farklı sıcaklıklarda (4-24°C) muhafaza edilmiştir. Hareketliliğin, karaca mersin balığının yumurta inkübasyonu sıcaklığına en yakın olan 14°C’de 28. dakikaya kadar devam ettiği ve canlılığın %5 olduğu görülmüştür. Sperm canlılığının 14°C’de 8. dakikaya kadar %50’den fazla olduğu belirlenmiştir. Bu nedenle karaca mersini spermi sulandırıldıktan sonra 8. dakikaya kadar döllemede kullanılabilir.

Anahtar Kelimeler

• Sperm hareketliliği,Karaca mersini,sıcaklık,Üreme

Kaynakça

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