Balık Yemlerinde Kullanılan Farklı Protein Kaynaklarının Sibirya Mersini (Acipencer baerii Brandt 1869) Jüvenillerinin Proteaz Aktivitesi Üzerine Etkileri

Yıl 2016, , 0 - 0, 10.05.2016

Cemal Alptekin , Kaya Gökçek

https://doi.org/10.17693/yunus.66485

Öz

Bu çalışmada, Sibirya mersin balığı, A. baerii, pre-larvalarının kondisyon faktörü üzerine besleme protokolünün etkisi ve ticari karma yemlerde kullanılan farklı protein kaynaklarının jüvenillerin proteaz aktivitesi üzerine olası inhibisyon etkisi in vitro metotlar kullanılarak incelenmiştir. Canlı yemle besleme ve karma yeme geçiş dönemi, kondisyon faktörünü istatistiki olarak etkilemiştir (p<0.05). Çalışmada, proteaz aktivitesi üzerine en düşük inhibisyon değeri soya protein konsantresi (%14.45±1.58) ve balık unundan (15.34±3.85%) elde edilmiştir. En yüksek inhibisyon değerleri ise soya unu (%63.33±4.71) ve kan ununda (%66.67±8.02) ölçülmüştür. Balık unu ve soya ununun 1:1 kombinasyonunda ise, enzim aktivitesi nispeten daha yüksek miktarda etkilenmiştir (%31.85±10.50)(p<0.05).

Anahtar Kelimeler:  A. baerii, proteaz aktivitesi, protein kaynakları, inhibisyon etkisi

  

Abstract

The Effects of Different Protein Sources on Protease Activity of Siberian Sturgeon (Acipencer baerii Brandt 1869) Juveniles

In the present study, the effect of feeding regime on condition coefficients and the inhibitory effects of different protein sources on early life proteases of Siberian sturgeon juveniles were in vitro tested. Feeding with live prey and transition period to artificial feed significantly affected the condition coefficients (p<0.05). In the study, the minimum inhibitory effect were obtained from soybean protein concentration (14.45±1.58%) and fish meal (15.34±3.85%). Also, the highest inhibitory effect was obtained from soy bean meal (63.33±4.71%) and blood meal (66.67±8.02%). The enzyme activity was relatively affected higher by dual combinations between fish meal and soybean meal (1:1) (31.85±10.50 %)(p<0.05).

Keywords:  A. baerii, protease activity, protein sources, inhibition effect

 

Kaynakça

• Alarcon, F. J., Moyano, F. J., Diaz, M., Fernandez-Diaz, C. ve Yufera, M. 1999. Optimization of the protein fraction of microcapsules used in feeding of marine fish larvae using in vitro digestibility techniques. Aquaculture Nutrition, 5: 107-113.
• Babaei, S. S., Kenari, A. A., Nazari, R. ve Gisbert, E. 2011. Developmental changes of digestive enzymes in Persian Sturgeon (Acipencer percius) during larval ontogeny. Aquaculture, 318: 138-144.
• Bagenal, T.B. 1978. Methods for the assessment of fish production in fresh waters. Blackwell Scintific Publication, Oxford, pp. 101-136.
• Bradford, M. M. 1976. A rapid sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dry binding. Anal. Biochem., 72, 248-254.
• Faulk, C. K., Benninghoff, A. D ve, Holt, G. J. 2007. Ontogeny of the gastrointestinal tract and selected digestive enzyme in cobia Rachycentron canadum (L.). J. Fish Biol. 70:567-583.
• Furne, M., Hidalgo, M. C., Lopez, A., Garcia- Gallego, M., Morales, A. E., Domezain, A., Domezaine, J. ve Sanz, A. 2005. Digestive enzyme activities in Adriatic sturgeon Acipencer naccarii and rainbow trout Oncorhynchus mykiss. A comperative study. Aquaculture, 250: 391-398.
• Garcia-Carreno, F. L. 1996. Proteinase inhibitors. Trends Food Sci. Technol., 7: 197-204.
• Gawlicka, A., Herold, M. A., Barrows, F. T., de le Noue, J. ve Hung, S. S. O. 2002. Effect of dieatary lipids on growth, fatty acid composition, intestinal absorbtion and hepatic storage in white sturgeon (Acipencer transmontanus) larvae. J. Appl. Icthyol., 18: 673-681.
• Gökçek, K., Szabo, T., Alptekin, C., Kurt, R., Töre, Y. ve Urbanyi, B. (Yayınlanmamış veri). The Effect of Feeding Regime and Inhibitory of Different Feed Ingredients on Proteases of Russian Sturgeon, Acipencer gueldenstaedtii Brandt&Ratzenburg, 1833, at Early Life Stages.
• Kim, B. G., Divakaran, S., Brown, C. L. ve Ostrowski, A. C. 2001. Comparative digestive enzyme ontogeny in two larval fishes: Pacific threadfin (Polydatilus sexfilis) and bluefin trevally (Caranx melampygus). Fish Physiol. Biochem. 24: 225-241.
• Kurt, R. ve Gökçek, K. (Yayınlanmamış veri). Ticari Protein Kaynaklarının Çuka Mersini, Acipencer ruthenus Linneaus 1758, Jüvenillerinin Proteaz Aktivitesi Üzerine Etkileri.
• Napora- Rutkowski, L., Kamaszewski, M., Bielawski, W., Ostaszewska, T. ve Wegner, A. 2009. Effects of starter diets on pancreatic enzyme activity in juvenile starlet (Acipencer ruthenus). The. Isr. Jour. Aqua.-Bamidgeh, 61 (2): 143-150.
• Noori, F., Gilbert, V. S. ve Sorgeloos, P. 2012. Preliminary study on the activity of protease enzymes in Persian sturgeon (Acipencer persicus Borodin, 1897) larvae in response to different diets: effects on growth and survival. Aquaculture Research, 43, 198-207.
• Sanz, A., Llorente, J. L., Furne, M., Ostos-Garrido, V., Carmona, R., Domezain, A. ve Hidalgo, M. C. 2011. Digestive enzymes during ontogeny of the sturgeon Acipencer naccarii: intestine and pancreas development. J. Appl. Ichthyol., 27: 1139-1146.
• Segner, H., Rösch, R., Verreth, J. ve Win, U. 1993. Larval nutrition physiology: studies with Clarias gariepinus, Coregonus lavaretus and Scophthalmus maximus. J. World Aqua. Soc. 24: 121-134.
• Walter, H. E. 1984. Proteinases: methods with hemoglobin, casein and azocoll as sub-strates, In: Methods of Enzymatic Analysis (Bergmeyer, H.J. ed.), 5, 270-277.
• Willot, P., Sabeau, L., Gessner, J., Arlati, G., Bronzi, P., Gulyas, T. ve Berni, P. 2001. Sturgeon farming in Western Europe: recent developments and perspectives. Aquat. Living Resourc., 14: 367-374.
• Zambonino Infante, J. L. ve Cahu, C. L. 2001. Ontogeny of the gastrointestinal tract of marine fish larvae. Comp. Biochem. Physiol. C, 130: 477-487.