Araştırma Makalesi
Türkiye'de Kültür Şartlarında Büyütülen Rus Mersin Balıklarından (Acipenser gueldenstaedtii) Sperm Alınması ve Muhafazası
Öz
Bu
çalışmada bütün dünyada nesilleri azalan Asipenceridae familyasının Karadeniz
ve ona bağlı nehirlerde yaşayan Rus mersininden kültür şartlarında sperm
alınması ve cryopreservasyonu çalışılmıştır. Semen abdominal masaj ile
toplanmıştır. Çalışmada
spermlerin zamana bağlı hız ve hareketleri 5 kategoride değerlendirilmiştir. Soğutma
0 ile -4°C arasında 3°C/dakika ve -4 ile
-10 °C arasında 5°C uygulanmıştır. Taze
semenler %8 DMSO Karyoprotectantı kullanılarak 5 farklı ekstendır ile -196
°C’de sıvı azot içinde dondurulmuştur. Spermatokrite oranları %65 ile %91
arasında değişen spermlerin pH’sı ortalama 8.0 olmuştur. Dondurmadan 24 saat
sonra sperm örnekleri 32°C su içinde 2 dakika düzenli çalkalanarak çözülmüş ve spermleri
harektlendirmek için su ilave edilmiştir. Taze spermlerin su ile
aktivasyonundan 10 dakika sonra hızları ve hareketlilikleri sırasıyla ++ ve %20
olarak belirlenmiştir. Elde ettiğimiz sonuçlar E2 ekstenderinin diğerlerinden
daha iyi olduğunu göstermiş ve Mersin balıklarının semeninin soğuk
muhafazasında faydalı bir ekstendir olabilir.
Anahtar Kelimeler
Kaynakça
- Alavi, S.M.H., Cosson, J. and Kazemi, P. 2004. Semen characterristics in Acipenser persicus in relation to sequential striping. J. Appl. Ichthyol., 22 (Suppl. 1): 400-405.
- Alavi, S.M.H., Cosson, J. and Kazemi, P. 2004. Semen characterristics in Acipenser persicus in relation to sequential striping. J. Appl. Ichthyol., 22 (Suppl. 1): 400-405.
- Aydın, İ,Karadeniz kalkan balığı (Psetta maxima Linnaeus, 1758) yumurta kalitesinin blastomer morfolojisi ile tahmin edilmesi ve triploid uygulamasının yumurta kalitesine etkilerinin belirlenmesi,120,Recep Tayip Erdoğan Üniversitesi Fen Bilimleri Enstitüsü,Rize,Turkish,2008
- Billard, R., Cosson, J., Noveiri, S.B. and Pourkazemi, M,Cryopreservation and short-term storage of sturgeon sperm, a review,1 – 9,English,2004,236
- Burtsev, I.A. and Serebryakova, A.V,First results of sturgeon sperm cryopreservation,94-100,E.V. Vladimirskaya,VNIRO,1969
- Chebanov, M.S., Savelyeva, E.A., Galich, E.V. and Chmir, Y.N.,Ecolocical- Biotecnological Problems in Sturgeon Culture in the Sea of Azov Basin,Trabzon,1998,September
- Chebanov, M.S., Savelyeva, E.A., Galich, E.V. and Chmir, Y.N.,Stock enhancement and conservation culture of sturgeons: problems and prospects,316-316,2004,65
- Ciereszko, A., Toth, G.P., Christ, S.A. and Dabrowski, K,Effect of cryopreservation and theophylline on motility characteristics of lake sturgeon (Acipenser fulvescens) spermatozoa,665-672,1996,45
- Çelikkale, M.S., Timur, M., Memis D. and Ercan, E.,A Study on Acclimation to the Cold Water of Juveniles Russian Sturgeon (A. gueldenstaedtii),137-140,2002,2
- Çelikkale M.S., Memis D., Ercan E. and Çağıltay, F,Growth performance of juvenile Russian sturgeon (Acipenser gueldenstaedtii Brandt& Ratzenburg, 1833) at two stocking densities in net cages,14-18,2005,21
- Dreanno, C., Suquet, M., Quemener, L., Cosson, J., Fierville, F., Normant, Y. and Billard, R.,Cryopreservation of turbot (Scophthalmus maximus) spermatozoa,589– 603,1997,48
- Glogowskia, J., Kolmanb, R., Szczepkowskib, M., Horva´thc, A´., Urba´nyic, B., Sieczyn´skia, P., Rzemienieckid, A., Domagalad, J., Demianowicza, W., Kowalskia, R. and Ciereszko, A,Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol,367-373,2002,211
- Horváth, Á., Wayman, W.R., Urbányi, B., Ware, K.M., Dean, J.C. and Tiersch, T.R.,The relationship of the cryoprotectants methanol and dimethyl sulfoxide and hyperosmotic extenders on sperm cryopreservation of two North-American sturgeon species,243– 251,2005,247
- Kopeika, E.F., Williot, P. and Goncharov, B.F,Cryopreservation of Atlantic sturgeon Acipenser sturio L., 1758 sperm: First results and associated problems,167-173,2000,16
- Kopeika, E. F, Kopeika, J. and Zhang, T,Cryopreservation of Fish Sperm, Methods in Molecular Biology,203-219,J.G. Day and Stacey G.N.,2007
- Lahnsteiner, F., Berger, B., Horvath, A. and Urbanyi, B.,Studies on the semen biology and sperm cryopreservation in the sterlet, Acipenser ruthenus L,519 – 528,2004,35
- Linhart, O., Mims, S.D. and Shelton, W.L.,Motility of spermatozoa from Shovelnose sturgeon, Scaphirhynchus platorynchus, and Paddlefish, Polyodon spathula,902-909,1995,47
- LLinhart, O., Mims, S. D., Gomelsky, B. Cvetkova, L. I., Cosson, J., Rodina, M., Horvath, A. and Urbanyi, B,Effect of cryoprotectants and male on motility parameters and fertilization rate in paddlefish (Polyodon spathula) frozen-thawed spermatozoa.,389–394,2006,22
- Liu, L., Wei, Q., Guo, F., Zhang, J. and Zhang, T,Cryopreservation of Chinese sturgeon (Acipenser sinensis) sperm,384–388,2006,22
- Memiş, D., Çelikkale, M.S. and Ercan, E,Effects of different diets on the growth performance and body composition of Russian sturgeon (A. gueldenstaedtii).,287–290,2006,22
- Memiş, D., Ercan, E., Çelikkale, M.S., Timur, M. and Zarkua, Z.,Development of Russian sturgeon (A.gueldenstaedtii) larvae from fertilized eggs to artificial feeding,International Workshop on Advanced Techniques in Sturgeon Fish Larviculture, Artemia,2007,Aquatic Animals Research Institute, Urmia University
- Mims, S.D,Evaluation of activator solutions, motility, duration, and short-term storage of paddlefish spermatozoa,224–229,1991,22
- Mirzoyan, A.V., Nebesikhina, N.A., Voynova, N.V. and Chistyakov, V.A,Preliminary results on ascorbic acid and lysine suppression of clastogenic effect of deep-frozen sperm of the Russian sturgeon (Acipenser gueldenstaedtii),374–378,2006,29
- Mounib, M.S,Cyrogenic preservation of fish and mammalian spermatozoa,13-18,1978,23
- Noveiri, S.B., Alipour, A. and Pourkazemi, M,Sperm morphometry, density and spermatocrit study in Persian sturgeon (Acipenser persicus),380-383,2006,22
- Rurangwa, E., Kime, D.E., Ollevier, F. and Nash, J.P,The measurement of sperm motility and factors affecting sperm quality in cultured fish,1-28,2004,234
- Vlasenko, A.D., Pavlov, A. V. and Vasil’ev, V.P,Acipenser gueldenstaedti Brandt, 1833,294-345,J. Holcík,156-200 AULA. Wiesbaden, Germany,The Freshwater Fishes of Europe. General Introduction to Fishes, Acipenseri-formes
Semen Collection and Cryopreservation of Russian Sturgeon (Acipenser gueldenstaedtii) Reared in Turkey
Öz
In this research, milt collection and cryopreservation with different extenders in endangered Russian sturgeon (Acipencer gueldenstaedtii) which lives Black Sea and its tributary rivers were studied. The semen was collected by abdominal massage. Sperm motility classified to five categories. Cooling were carried out as follow; 3°C/min between 0 and -4 and 5°C/min between -4 and -10. Sperm were frozen using 5 different extenders and with 8% DMSO as cyroprotectant at -196 °C in liquid nitrogen. Spermatocrite ratios range from 65% to 91%. Sperm pH was determined 8.0. After 24 hours the deep frozen sperm samples were thawed by quickly immersing them in a water bath at 32 ºC under constant shaking for about 2 min, and freshwater was added to stimulate the sperm motility. In ten minutes after activation, the velocity and motility of sperm were ++ and 20%. Our results indicate that E2 extender was better than the others and may useful extender for cryopreservation of sturgeon semen
Anahtar Kelimeler
Kaynakça
- Alavi, S.M.H., Cosson, J. and Kazemi, P. 2004. Semen characterristics in Acipenser persicus in relation to sequential striping. J. Appl. Ichthyol., 22 (Suppl. 1): 400-405.
- Alavi, S.M.H., Cosson, J. and Kazemi, P. 2004. Semen characterristics in Acipenser persicus in relation to sequential striping. J. Appl. Ichthyol., 22 (Suppl. 1): 400-405.
- Aydın, İ,Karadeniz kalkan balığı (Psetta maxima Linnaeus, 1758) yumurta kalitesinin blastomer morfolojisi ile tahmin edilmesi ve triploid uygulamasının yumurta kalitesine etkilerinin belirlenmesi,120,Recep Tayip Erdoğan Üniversitesi Fen Bilimleri Enstitüsü,Rize,Turkish,2008
- Billard, R., Cosson, J., Noveiri, S.B. and Pourkazemi, M,Cryopreservation and short-term storage of sturgeon sperm, a review,1 – 9,English,2004,236
- Burtsev, I.A. and Serebryakova, A.V,First results of sturgeon sperm cryopreservation,94-100,E.V. Vladimirskaya,VNIRO,1969
- Chebanov, M.S., Savelyeva, E.A., Galich, E.V. and Chmir, Y.N.,Ecolocical- Biotecnological Problems in Sturgeon Culture in the Sea of Azov Basin,Trabzon,1998,September
- Chebanov, M.S., Savelyeva, E.A., Galich, E.V. and Chmir, Y.N.,Stock enhancement and conservation culture of sturgeons: problems and prospects,316-316,2004,65
- Ciereszko, A., Toth, G.P., Christ, S.A. and Dabrowski, K,Effect of cryopreservation and theophylline on motility characteristics of lake sturgeon (Acipenser fulvescens) spermatozoa,665-672,1996,45
- Çelikkale, M.S., Timur, M., Memis D. and Ercan, E.,A Study on Acclimation to the Cold Water of Juveniles Russian Sturgeon (A. gueldenstaedtii),137-140,2002,2
- Çelikkale M.S., Memis D., Ercan E. and Çağıltay, F,Growth performance of juvenile Russian sturgeon (Acipenser gueldenstaedtii Brandt& Ratzenburg, 1833) at two stocking densities in net cages,14-18,2005,21
- Dreanno, C., Suquet, M., Quemener, L., Cosson, J., Fierville, F., Normant, Y. and Billard, R.,Cryopreservation of turbot (Scophthalmus maximus) spermatozoa,589– 603,1997,48
- Glogowskia, J., Kolmanb, R., Szczepkowskib, M., Horva´thc, A´., Urba´nyic, B., Sieczyn´skia, P., Rzemienieckid, A., Domagalad, J., Demianowicza, W., Kowalskia, R. and Ciereszko, A,Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol,367-373,2002,211
- Horváth, Á., Wayman, W.R., Urbányi, B., Ware, K.M., Dean, J.C. and Tiersch, T.R.,The relationship of the cryoprotectants methanol and dimethyl sulfoxide and hyperosmotic extenders on sperm cryopreservation of two North-American sturgeon species,243– 251,2005,247
- Kopeika, E.F., Williot, P. and Goncharov, B.F,Cryopreservation of Atlantic sturgeon Acipenser sturio L., 1758 sperm: First results and associated problems,167-173,2000,16
- Kopeika, E. F, Kopeika, J. and Zhang, T,Cryopreservation of Fish Sperm, Methods in Molecular Biology,203-219,J.G. Day and Stacey G.N.,2007
- Lahnsteiner, F., Berger, B., Horvath, A. and Urbanyi, B.,Studies on the semen biology and sperm cryopreservation in the sterlet, Acipenser ruthenus L,519 – 528,2004,35
- Linhart, O., Mims, S.D. and Shelton, W.L.,Motility of spermatozoa from Shovelnose sturgeon, Scaphirhynchus platorynchus, and Paddlefish, Polyodon spathula,902-909,1995,47
- LLinhart, O., Mims, S. D., Gomelsky, B. Cvetkova, L. I., Cosson, J., Rodina, M., Horvath, A. and Urbanyi, B,Effect of cryoprotectants and male on motility parameters and fertilization rate in paddlefish (Polyodon spathula) frozen-thawed spermatozoa.,389–394,2006,22
- Liu, L., Wei, Q., Guo, F., Zhang, J. and Zhang, T,Cryopreservation of Chinese sturgeon (Acipenser sinensis) sperm,384–388,2006,22
- Memiş, D., Çelikkale, M.S. and Ercan, E,Effects of different diets on the growth performance and body composition of Russian sturgeon (A. gueldenstaedtii).,287–290,2006,22
- Memiş, D., Ercan, E., Çelikkale, M.S., Timur, M. and Zarkua, Z.,Development of Russian sturgeon (A.gueldenstaedtii) larvae from fertilized eggs to artificial feeding,International Workshop on Advanced Techniques in Sturgeon Fish Larviculture, Artemia,2007,Aquatic Animals Research Institute, Urmia University
- Mims, S.D,Evaluation of activator solutions, motility, duration, and short-term storage of paddlefish spermatozoa,224–229,1991,22
- Mirzoyan, A.V., Nebesikhina, N.A., Voynova, N.V. and Chistyakov, V.A,Preliminary results on ascorbic acid and lysine suppression of clastogenic effect of deep-frozen sperm of the Russian sturgeon (Acipenser gueldenstaedtii),374–378,2006,29
- Mounib, M.S,Cyrogenic preservation of fish and mammalian spermatozoa,13-18,1978,23
- Noveiri, S.B., Alipour, A. and Pourkazemi, M,Sperm morphometry, density and spermatocrit study in Persian sturgeon (Acipenser persicus),380-383,2006,22
- Rurangwa, E., Kime, D.E., Ollevier, F. and Nash, J.P,The measurement of sperm motility and factors affecting sperm quality in cultured fish,1-28,2004,234
- Vlasenko, A.D., Pavlov, A. V. and Vasil’ev, V.P,Acipenser gueldenstaedti Brandt, 1833,294-345,J. Holcík,156-200 AULA. Wiesbaden, Germany,The Freshwater Fishes of Europe. General Introduction to Fishes, Acipenseri-formes
Toplam 27 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Haziran 2012 |
| Yayımlandığı Sayı | Yıl 2012 Cilt: 2012 Sayı: 3 |
