Lipid Peroksidasyon Oluşumunun Engellenmesi Üzerine Verapamilin Etkisi

Yıl 1998, Cilt: 4 Sayı: 1-2, 1 - 4, 14.06.1998

Burhanettin Baydaş Fahri Bayıroğlu Tahir Kahraman Zahid Ağaoğlu Ramazan Üstün

Öz

Bıı çalışma, karbontetrakloriir CCI4 uygulaması ile lipid peroksidasyon oluşumu ve hücre içi kalsiyum artışını bloke eden verapamil’in lipidperoksidasyonu önleme etkisi araştırılmak amacıyla planlandı. Bunun için toplam 24 adet albino cinsi rat kullanıldı. Ratlar üç gruba ayrıldı. Birinci gruba sadece verapamil 5 mg/kg, i.p. , ikinci gruba sadece CCI4 0.4 ml/kg, s.c. ve iıçüncii gruba ise verapamil uygulandıktan bir saat sonra CCI4 uygulandı. Bu uygulamalar iki gün süre ile tekrarlandı, iki giın sonra rotlardan eter anestezisi altında kan alınarak malondialdehit MDA , rediıkte glutatyon GSH , seruloplazmin ve vitamin C seviyeleri ölçüldü. Karbon tetrakloriir uygulanan ratlarda, malondialdehit miktarı diğer gruplara oranla yüksek bulundu. Gruplar arası karşılaştırma yapıldığında; CCl4 ile verapamil grupları ve CCI4 ile CCl4+verapamil grupları arasında belirgin bir farkın mevcut olduğu ve bunun istatistiki olarak anlamlı olduğu belirlendi P0.05 . Canlı organizmada antioksidan sistemin elemanları olarak bilinen GSH, seruloplazmin ve vitamin C değerleri açısından gruplar arasındaki farkların önemli olmadığı gözlendi P>0.05 . Malondialdehit miktarları göz önüne alındığında, bir kalsiyum kanal blokörii olan verapamilin, CCI4 ile oluşturulan lipid peroksidasyon oluşumunu engelleyebileceği tespit edildi

Anahtar Kelimeler

Verapamil, Lipid peroksidasyon, Glutatyon, Seruloplazmin, Vitamin C, CCI4

The Effect of Verapamil on Preventioıı of Lipid Peroxidation Formation

Öz

The Effect of Verapamil on Preventioıı of Lipid Peroxidation Formation The purpose of this investigation was to fınd out yvhether CCI4 cause lipid peroxidation generation and verapamil ,calcium channel blocker, prevents lipid peroxidation generation. Twenty-four Wistar Albino rats were randomly divided inîo three groups. Following a week adaptation period, the rats in the first group were injected verapamil i.p. ,5 mg/kg , the secoııd group was treated by CCI4 and the third group were treated by verapamil one hour later following CCl4 treatment. Treatment s in ali groııps were repeated t w o consecııtive days. The end of the treatment ,the bloods with EDTA were collected from the hc:ırts under the ether anesthesia. The analyses of MDA, GSH, seruloplasmin and vitamin C were performed. MDA amounts were elevated in the rats treated with only CCI4 in comparison with the other groups. The amount of the MDA yvere statistically differed betvveen CCI4 and verapamil groups and between CCI4 and CCI4 + verapamil groups. Bul the difference were not statistically important between verapamil and CCI4 plus verapamil groups. At the same time, the levels of GSH ,seruloplasmin and vitamin C ,antioxidant system elements,\vere not different among the groups. The results demonstrate that the treatment of verapamil, a Ca2+ channel blocker, was effective in normalizing lipid peroxidation induced by CCI4

Anahtar Kelimeler

Verapamil, Lipid peroxidation, Glutathione, Ascorbic acid, Ceruloplasmin, CCl4

Kaynakça

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