Elektrik Alanın, Rat Eritrosit ve Dokularındaki Antioksidan Enzim Süperoksid Dismutaz, Glutatyon Peroksidaz Aktiviteleri, Lipid Peroksidasyonu ve Glutatyon Seviyelerine Etkisi

Yıl 1998, Cilt: 4 Sayı: 1-2, 65 - 71, 14.06.1998

Tahir Kahraman Haluk Testereci

Öz

Sunulan çalışmada doğru DC , alternatif akım AC ile trafo merkezinde yüksek voltaj altında TR sırasıyla, 0.74 kV/m, 0.81 kV/m ve 50 kV/m şiddetinde elektrik alan etkisine bırakılan rotların eritrosit ve dokularında, süperoksid dismutaz SOD , glutatyon peroksidaz GSH-Px aktiviteleri, lipid peroksidasyonu malondialdehid MDA ve glutatyon GSH düzeyleri araştırıldı. 44 genç erkek rat, 11 hayvandan oluşan 4 gruba ayrıldı. Birinci grup kontrol olarak alındı. Diğer üç grup 40 gün boyunca günde 24 saat süreyle farklı şiddetteki elektrik alan etkisine bırakıldı. Tüm ratlardan 20. ve 40. günlerde kan örnekleri alındı. 44. günde karaciğer ve beyin dokuları kesilerek çıkartıldı. MDA, GSH, SOD ve GSH-Px kan ve dokulardaki düzeyleri, spektrofotometrik olarak ölçüldü. Kan MDA düzeyi, DC ve AC gruplarında kontrol grubuna göre artarken, kan glutatyon düzeyi bütün gruplarda önemli oranda azaldı p

Anahtar Kelimeler

Elektrik alan, Antioksidanlar, Malondialdehid, Eritrosit, Doku

Effect of Electric Field on Activities of Antioxidaııt Enzymes SOD, GSH-Px , Lipid Peroxidation MDA and Glutathioııe Levels in Rat Erythrocytes and Tissues

Öz

Effect of Electric Field on Activities of Antioxidaııt Enzymes SOD, GSH-Px , Lipid Peroxidation MDA and Glutathioııe Levels in Rat Erythrocytes and Tissues The main aim of this study was to investigate activity of SOD, GSH-Px as w e ll as MDA and GSH levels in the rat erythrocytes and tissues exposed to ele ctric field of dire ct current DC , alternating cıırrent AC and Van Transformer Çenter TR with intensity of 0.74 kV/m, 0.81 kV/m and 50 kV/m respectively. Forty-four adult male rats were divided into four groups of 11 animals. The first group was used as the control KN . The other groups were exposed to three different electric fields described abovefor 24 hours till 40th day. Blood samples were collected on 20th and 40rh days. Liver and brain tissues were excised on the 44th day. MDA, GSH, SOD and GSH-Px w e re determined on both tissues and blood samples by means of spectrophotometer. In blood, MDA levels in DC and AC groups were found to be signifıcantly increased comparing to other groups p

Anahtar Kelimeler

Electric field, Erythrocytes, Malondialdehyde, Antioxidcınt, Tissue.

Kaynakça

• 1. Sümer Ş, Baldemir F and Çelik T: An Investigation on the Effects of Electric Fields on Celi Division in Barley (Hordeum vulgare L.), FÜ Fen ve Müh. Bil. Der. 7(1): 171-178 (1995).
• 2. Billings CE: Fiziksel Ajanların Etkileri, N. Zengin(Ed) Sodemans Fizyopatolojisi, 1164-1190, Türkiye Klinikleri Yayınevi, Ankara (1992).
• 3. Flink EB and Prasad AS: Kimyasal Ajanlar ve Hastalık, S. Turgut(Ed) Sodemans Fizyopatolojisi, 1191 - 1208, Türkiye Klinikleri Yayınevi, Ankara (1992).
• 4. Dowman R, Wolpaw JR, Seegal RF and Murti SS: Chronic Exposure of Primates to 60 Hz Electric and Magnetic Fields: III. Neurophysiologic Effects, Bioelectromagnetics, 10:303 - 317(1989).
• 5. Theriault G, Goldberg M, Miller AB, Armstrong B, Guenel P, Deadman J, İmbernon E, To T, Chevalier A, Cry D and Wall C: Cancer Risks Associated with Occupational Exposure to Magnetic Field Among Electric Utility Workers in Ontoria and Quebec, Canada and France: (1970- 1989), American J. Epidemiology, 139(6):550 - 572 (1994).
• 6. Kavet R and Black R: Electrical and Magnetic Fields: Health Reseach Agıicultural Engineeıing, 66( 1 ):21—24 (1985).
• 7. Korur E: Elektrik Hatları Sağlığa Zararlı mı? A. Coghlan (Ed), In " New Scientist" Bilim ve Teknik Derg., 20:6-8(1992).
• 8. Pentland AP: Active Oxygen Mechanisms of UV Inflammation, Free Radicals in Diagnostic Medicine, D.Amstrong(Ed), pp. 87-97, Plenum Press, New York (1994).
• 9. Atalay NS, Güler G, Koz M ve Gönül B: Elektrik Alanın Böbreküstü Bezi Malondialdehid (MDA) Seviyesine Etkisi, Türkiye Tıp Derg., 1(39): 161- 167 (1994).
• 10. Wolpaw JR, Seegal RF and Dovvman R: Chronic Exposure of Primates to 60-Hz Electric and Magnetic Fields: I. Exposure System and Measurements of General Health and Performance, Bioelectromagnetics, 10: 277-288 (1989).
• 11. Anon. Randox Lab. Lmd. Ransod Süpeıoxide Dismutase Enzim Kiti, (1996).
• 12. Anon. Randox Lab. Lmd., Ransel Glutathione Peroxidase Enzim Kiti, (1996).
• 13. Sushil JK, Mcvie R, Duett J and Herbst JJ: Erythrocyte Membrane Lipid Peroxidation and Glycosylated Hemoglobin in Diabetes, Diabetes, 38:1539-1543 (1989).
• 14. Xia E, Rao G, Remmen HV, Heydari AR and Richardson A: Activities of Antioxidant Enzymes in Various Tissues of Male Fischer 344 Rats aıe Altered by Food Restriction, J. Nutr. 125:195-201 (1994).
• 15. Paglia DE and Valentine WN: Studies on The Quantitative and Qualitative Chaıacterization of Erythrocyte Glutathione Peroxidase, J. Lab. Clin. Med., 70(1): 158-168 (1967).
• 16. Beutler E, Dubon O and Kelly BM: Improved Method for The Determinatioıı of Blood Glutathione, J. Lab. Clin. Med., 61:882-888 (1963).
• 17. Rizzi R, Caroli A, Bolla P, Acciaioli A and Pagnacco G: Variability of Reduced Glutathione Levels in Massese Ewes and Its Effect on Daily Milk Pıoduction, J. of Dairy Research, 55:345-353 (1988)
• 18. Ball CR:Estimation and Identification of Thiols in Rat Spleen After Cysteine or Glutathione Treatment, Pelevance to Protection Against Nitrojen Mustards, Biochem. Pharmac., 15: 809-816. Pergamon Press Lmd. (1966).
• 19. Tiftik AM: Biüret Metoduyla Total Protein Tayini, Klinik Biyokimya, pp. 291-292, Mimoza Yayınları, Konya (1996).
• 20. Cochran WG and Cox GM: Experimental Desing, John Wiley£Jons, Ne w York (1950)
• 21. Anon. SPSS for Windows, Release 6.1 Standart Version, USA, (1994).
• 22. Snedecor G, Wand Cohran WG: Statistical Methods, Iowa State University, Press Ames, pp. 1-503, USA (1989).
• 23. Akgül A: Tıbbi Araştırmalarda İstatistiksel Analiz Teknikleri SPSS Uygulamaları, Yüksek Öğretim Kurulu Matbaası, Ankara (1997)
• 24. Yagi K:Lipid Peroxides and Related Radicals in Clinical Medicine, Free Radicals in Diagnostic Medicine, D. Armstrong(Ed), pp. 17-27, Plenum Press, New York (1994).
• 25. Halliwel B and Gutteridge JM: Lipid Peroxidation, Oxygen Radicals, Celi Damage, and Antioxidant Therapy, The Lancet, 23:1396-1397 (1984).
• 26. Janssen YMW, Houten BV, Bonn PJA and Mossmon BT:Biology of Disease, Celi and Tissue Responses to Oxidative Damage, Lab. Invest., 69(3):261-274 (1993).
• 27. Akkuş I: Serbest Radikaller ve Fizyopatolojik Etkileri, Mimoza Yayınları, Konya (1995).
• 28. Özdem SS ve Şadan G: Serbest Oksijen Radikallerinin Oluşumu ve Klinik Açıdan Önemi, Akdeniz Ü. Tıp Fak. Der. XI (l):63-71 (1994).
• 29. Romodanova EA, Paranich AV and Chaikina LA: Effect of Chronic Effect of The Electrostatic Field on Various Biochemical lııdicators of The Tissues, Fiziol Zh., May-Jun., 36(3):30-34 (1990).
• 30. Levshin IV: Permeability of Erhytrocyte Membranes from Peripheral Blood After Exposure to Low- Frequency Alternating Electromagnetic Field, Patologicheskaya Fiziolgiya i Eksperimentalnaya Terapiya, 1:17-19 (1990).
• 31. Meister A and Anderson ME: Glutathione, Ann. Rev. Biochem. 52: 711 - 760 (1983).
• 32. Güler G, Atalay NS, Altan N ve Yavuz Ö: Süperoksid Dismutaz Aktivitesini Artırmada Yeni Bir Etken: Elektrik Alanlar, Türk Fizyoloji Bil. Derneği 21.Ulusal Kong., Ankara, 24-28.Eylül. 1995.
• 33. Dinçer S, Koz E, Gönül B, Güler G ve Atalay NS: Elektrik Alanın Askorbik Asit ve Malondialdehid Düzeyleri Üzerine Etkisi, T. Fizyolojik Bil. Der. 21.Ulusal Kong., Ankara 24-28.Eylul.1995.