Zeranol İmplante Edilen Erkek Kuzuların İç Salgı Bezleri ile Genital Organlarındaki Patolojik Bulgular

Yıl 1997, Cilt: 3 Sayı: 1, 11 - 25, 15.06.1997

M.yavuz Gülbahar Cemalettin Köküuslu

Öz

Bu çalışmada zeranol Ralgro® implante edilen erkek kuzuların genital organları ile iç salgı bezlerinde meydana gelen değişiklikler değerlendirilmiştir. Çalışmada 32 adet, 3 aylık ±10 gün erkek, Akkaraman ırkı kuzu kullanıldı. Deneme grubundaki kuzuların kulak derisi altına sırasıyla 12 I. grup , 24 mg II. grup ve 96 mg Ill.grup zeranol implante edilerek, 33 gün sonra nekropsileri yapıldı. Adenohipofizde asidofil hücrelerin sayı ve büyüklük olarak arttığı, bazofil hücrelerin ise sayı ve büyüklük olarak azaldığı dikkati çekti. Deneme süresinin sonunda tiroid bezlerinin aktivasyon durumlarının bazı kuzularda azaldığı, bazılarının etkilenmediği veya aktivasyonun arttığı gözlendi. Adrenal korteks kalınlığını ise önemli şekilde artırdığı dikkati çekti p

Anahtar Kelimeler

Zeranol, Kuzu, Genital sistem, Endokrin sistem

Pathological Findings in the Endocrine Glands and Genital Organs of the Male Lambs implanted with Zeranol

Öz

This experiment yvas conducted to examine the changes on the endocrine glands and reproductive organs in zeranol Ralgro® implanted male lambs. Three-months old thirtytyvo male Akkaraman lambs yvere used for this study. The treatment groups yvere implanted subcutaneously at the ear of the lambs yvith 12 mg Group I , 24 mg Group II and 96 mg Group III zeranol. The non-treatment group yvas served as control. After 33 days, all the lambs yvere necropsied. The acidophil cells shoyved a significant increase in number and size, hoyvever, there yvas a significant decrease in number and size of basophil cells in the pars distalls of pituitary glands. Thyroid gland activity in treated groups vvere noticed to increase in the fırst treatment and to decrease or remain unchanged in the second treatment in generally. In the treatment groups, a significant increase yvas observed in adrenal cortex yvidth över the Controls P

Anahtar Kelimeler

Zeranol, Lamb, Genital organs, Endocrine glands

Kaynakça

• I- Rico, A.G. Metabolism of endogenous and exogenous anabolic agents in cattle. J. Anim. Sci., 57: 226-232, 1983.
• Fara, G.M., Del Corvo, G., Bemuzzi, S., Bigatello, A, Di
• Pietro, C., Scaglioni, S. and Chiumello, G. Epidemic of breast enlargement in an
• İtallan school. Lancet, 2: 295-297, 1979.
• Scaglioni, S., Di Pietro, C., Bigatello, A and Chiumello, G.
• Breast enlargement at an itallan school. Lancet, i: 551-552, 1978.
• Bongiovanni, A.M. An epidemic of prematüre thelarche in
• Rodriguez, C.A.S. Environmental hormone contamination in Puerto Rico. N. Eng. J. Med., 310: 1741-1742, 1984.
• Rodriguez, C.A.S. and Toro-sola, M.A Anabolic steroids in meat and prematüre telarche. Lancet, 1: 1300, 1982.
• Lamming, G.E. Scientifıc report on anabolic agents in animal production. Vet. Rec., 122: 389-391, 1987.
• Sawyer, G. J. and Barker, D. J. Growth promotants in cattle in Australla. Aust. Vet. J., 65: 101-108, 1988.
• Sundlof, S.F. and Strickland, C. Zearalenone and zeranol: Potential residue problems in livestock. Vet. Hum. Toxicol., 28: . 42-250, 1986.
• in beef bulls: Scrotal sircumference, serving ability, semen characteristics, and
• pathologic changes of the reproductive organs. Am. J. Vet. Res., 48: 137-147, 1987.
• Beverly, J.R. Ralgro-Its mode of action. In: Proceedings of the meeting implanting for grovvth, 5-19, 1984.
• II- Hidy, P.H., Baldvvin, R.S., Greasham, R.L., Keith, C.L. and
• E. J. Oestrogen-induced changes in the genital taret of the male calf. Zbl. Vet.
• Med. A, 18: 717-730, 1971.
• McMullen, J.R. Zearalenone and some derivates: Production and biological
• activities. Adv. Appl. Microbiol., 22: 59-82, 1977.
• Katzenellenbogen, B.S., Katzenellenbogen, J.A. and
• Mirocha, C.J. Histologic changes in the genital tracts of svvine fed estrogenic
• mycotoxin. Am. J. Vet. Res., 30: 551-556, 1969.
• Mordecai, D. Zearalenones: Characterization of the estrogenic potencies and
• receptor interactions of a series of fungal Ş-resorcylic acid lactones.
• Endocrinology, 105: 33-40, 1979.
• Trenkle, A Mechanism of action for the use of anabolics. In: E Meissonier, (e): Anabolics in Animal Productions, OIE, Paris, p 65- 73, 1983. 15: 497-503, 1967.
• Buttery, B.J., vemon, B.G. and Pearson, J.T. Anabolic agents- some thoughts on their mode of action. Proc. Nutr. Soc., 37: 311- 315, 1978.
• Azzall, G.R. Histochemical and ultrastructural modifıcations of mice endometrium, vagina and pituitary follovving zeranol treatment. Experientia, 33: 1638-1639, 1977.
• Kennedy, and N., Palmer (e): Pathology of Domestic Animals, Third edition,
• Academic Press, Orlando, Florida, USA, p 395, 1985.
• Rao veeramachaneni, D.N., Sherman, G.B., Floyd, J.G., Ott, R.S. and Hixon, J.E. Zeranol and estradiol induce similar lesions in the testis and epididymides of the prepubertal beef bull. Fundam. Appl. Toxicol., 10:73-81, 1988.
• A 15: 514-516, 1958.
• Rothenbacher, H., Wiggins, J.P. and Wilson, L.L.
• testosteron on Merino vVethers exposed to highly oestrogenic subterranean
• elover pasture. Aust. Vet. J., 63: 188-190, 1986.
• Pathological changes in endocrine glands and certain other tissues of lambs
• implanted with the synthetic grovvth promotant zeranol. Am. J. Vet. Res., 36: 1313-1317, 1975.
• Wiggins, J.P., Rothenbacher, H. and Wilson, L.L. Histologic evaluation of the effects of diethylstilbestrol and zeranol on certain lamb tissues. Am. J. Vet. Res., 41: 487-492, 1980.
• Çiftçi, M.K. and Kıran, M.M. Erkek Merinos kuzulara implante edilen zeranolun genital organlara etkisi üzerine histopatolojik incelemeler. S.Ü. Vet. Fak. Derg., 7: 16-22, 1990.
• Çiftçi, M.K., Deligözoğlu, F., Kaya, Z. ve Traş, B. Zeranol implante edilen pubertal dönemdeki esmer erkek ırk danaların testis, epididimis ve eklenti bezlerinde görülen histopatolojik değişiklikler. S.Ü. Vet. Fak. Derg., 6: 23-28, 1990.
• Luna, L.G. Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology, 3th Ed., McGraw-Hill Book Company, Nevv York, USA, 1968.
• Food and Drug Administration. Part 135b-New animal drugs for implantation or injection. Part 135g-Tolerances for residues of nevv animal drugs in food. Federal Register 35,(168), 13727-13728, 1970.
• Hassan, AH. S., Kamel, G. and El-Hammosi, F.F. The effect of zeranol implantation on some endocrine glands and gonads in fat- tailed lambs. l.The pituitary gland (Pars distalls) Z. Mikrosk. Anat. Forsch. (Leipz.), 95:634- 646, 1981.
• Wiggins, J.P., Rothenbacher, H., Wilson, L.L., Martin, R.J., Wangness, R.J. and Ziegler, J.H. Growth and endocrine responses of lambs to zeranol implants: Effects of preimplant growth rate and breed of sire. J. Anim. Sci., 49: 291-297, 1979.
• Ballachey, B.E., Miller, H.L., Jost, L.K. and Evenson, D.P. Flow cytometry evaluation of testicular and sperm cells obtained from bulls implanted with zeranol. J. Anim. Sci., 63: 995-1004, 1986.
• Godfrey, R.W., Randel, R.D. and Rouquette, F.M. Effect of zeranol on sexual development of cross bulls. J. Anim. Sci., 67: 1751- 1756, 1989.
• Gray, D.G., Unruh, J.A, Dikeman, M.E. and Stevenson, J.S. implanting young bulls vvith zeranol from birth to four slaughter ages: III. Growth performance and endocrine aspects. J. Anim. Sci., 63: 747-756, 1986.
• Junievvicz, P.E., Welsh, T.H. and Johnson, B.H. Effects of zeranol upon bovine testicular fimction. Theriogenology, 23: 565-582, 1985
• Staigmiller, R.B., Brovvnson, R.M., Kartcher, R.J. and Williams, J.H. Sexual development in beef bulls follovving zeranol implants. J. Anim. Sci., 60: 342-351, 1985.
• Floyd, J.G., Ott, R.S., Hixon, J.E., Rao veeramachneni, D.N., Willms, C.F. and Parett, D.G. Effects of zeranol implanted during a postvveaning vveight gain test on testicular, semen, and endocrine characteristics of bulls. Am. J. Vet. Res., 55: 556-560, 1994.
• Carr, W.R. and Land, R.B. Plasma luteinizing hormone levels and testis diameters of ram lambs of dififerent breeds. J. Reprod. Fertik, 42: 325- 333, 1975.
• Skinner, J.D., Booth, W.D., Rovvson, L.E.A and Karg, H. The post-natal development of the reproductive tract of the Suffolk ram, and changes in the gonadotrophin content of the pituitary. J. Reprod. Fert., 16:463-477, 1968.
• Deschamps, J.C., Ott, R.S., McEntee, K., Heath, E.H.,
• Kroes, R., Berkvens, J.M., Loendersloot, H.T. and Ruitenberg,
• Kurtz, H.J., Naim, M.E., Nelson, G.H., Christensen, C.M. and
• Mirocha, C.J., Christensen, C.M. and Nelson, G.H. Estrogenic
• Ruitenberg, E.J., Kroes, R. and Berkvens, J. Evaluation of the “prostate test” in checking the administration of oestrogens in the calf. Zbl. Vet. Med. A, 17: 351-357, 1970.
• Maxie, M.G. The Urinary System. In: K.V.F. Jubb, P.C.
• Udall, R.H. and Jensen, R. Studies on urolithiasis. II. The
• Gardner, J.J. and Adams, N.R. The effects of zeranol and
• Pryor, W.J. implantation of resorcylic acid lactone in cattle andsheep. Aust. Vet. J., 49: 593-594, 1973.
• Anonim . A grovving controversy. Vet. Rec., 138: 121, 1996.
• Lamming, G.E. Scientifıc report on anabolic agents in animal production. Vet. Rec., 122:389-391, 1987.