Farklı Sabunlaştırma Yöntemleri Kullanarak Kan Serumunda ve Sütte Karotin ve Vitamin A Tayini Üzerinde Çalışmalar

Yıl 1997, Cilt: 3 Sayı: 1, 27 - 30, 15.06.1997

Nurhayat Atasoy Hayati Çamaş

Öz

Bu çalışmada, soğuk ve sıcak sabunlaştırma yöntemleri kullanılarak kan serumunda ve sütte vitamin A ile total karotinlerin miktarları tayin edildi. Yirmi koyundan alınan kan serumunda, sabunlaştırma işlemi uygulanmaksızın yapılan tayinde ortalama vitamin A değeri 37.92 i 2.31 /Llg/100 ml, total karotin değeri 51.57 i 2.49 /Llg/lOO ml; soğuk sabunlaştırmada vitamin A 37.31 + 2.39 /l g/100 ml, total karotin 50.91 i 2.59 /Lig/100 ml; sıcak sabunlaştırmada ise vitamin A 37.87 + 2.28 /Lig/100 ml, total karotin de 51.06 +2.59 /Llg/lOO ml olarak bulundu. Sütteki değerler ise 20 inek sütünün anallzi sonunda ortalama vitamin A değerleri soğuk sabunlaştırma ile 54.09 +5.20 /Llg/lOO ml, sıcak sabunlaştırma ile 54.15+5.17 JLlg/lOO ml; total karotin değerleri de soğuk sabunlaştırmada 71.87+6.71 /Ll g/100 ml, sıcak sabunlaştırmada 71.89 + 6.72 /Llg/100 ml olarak tesbit edildi. Yapılan istatistiki hesaplamalar sonunda değişik işlemlerle elde edilen değerler arasında herhangi bir fark bulunmadı

Anahtar Kelimeler

Vitamin A, Total karotin, Sıcak sabunlaştırma, Soğuk sabunlaştırma, Süt ve serum

Studies on Vitamin A and Carotene Levels in Blood Serum and Milk by Using Different Saponification Methods

Öz

Studies on Vitamin A and Carotene Levels in Blood Serum and Milk by Using Different Saponification Methods In this study, by using cold and hot Saponification methods, the amounts of vitamin A and the total carotene in blood serum and milk has been determined. The total vitamin A value in blood serums taken from 20 sheep, yvıthout using saponification method, yvas 37.92 +2.31 /Llg/lOO ml, the total carotene value yvas 51.57 +2.49 /Llg/lOO ml; the vitamin A value in cold saponification yvas 37.31 +2.39 /Z g/100 ml, the total cartone value vvas 50.91 +2.59 /Llg/100 ml; as to the hot saponification, the vitamin A value yvas 37.87 +2.28 /Llg/lOO ml, and the total carotene yvas 51.06 + 2.59 /Llg/lOO ml. As to the values in milk; the average vitamin A values in milk taken from 20 coyvs, yvas 54.09 + 5.20 /Llg/lOO ml in cold saponification, yvas 54.15 +5.17 /Llg/100 ml in hot saponification, the total carotene values yvas 71.87 + 6.71 /Llg/lOO ml in cold saponification, yvas 71.89 +6.72 /Llg/lOO ml in hot saponification. There yvere not found any statistically difference betyveen values

Anahtar Kelimeler

Vitamin A, Total carotene, Cold saponification, Hot saponification, Milk and serum

Kaynakça

• Egemen A.: Vitaminlerin Sağlığımızdaki Önemi. Ankara, (1986).
• Adam R.C.: Vitaminler ve Antivtaminler. E.Ü. Ziraat Fak. Yay. 33, İzmir, (1960).
• Ersoy E., Bayşu N.: Biyokimya. A Ü. Vet.Fak.Yay., Ankara, (1986).
• Kaplan A, Pesce J.: Clinical Chemistry. Medical Research Laborotories. Ohio, 411- 550, (1989).
• Marks J. The Vitamines in Health and Diseases a Modem Reapracial. 42-49, (1968).
• Bingöl G. Biyokimya. A Ü. Eczacılık Fak. Yay., Ankara, (1978).
• Martin D., Mayes P., Rodwel V.: Harper’s Review of Biochemistry. 114, Lange Medical Publication, Callfomia, (1983).
• Moore T.: Vitamin A 18, 114-215, (1957).
• Wolf G.: Vitamin A in; RB Alfin-Slater. Human Nutrition. 3B, 97- 203, (1980).
• Pamell JP, Sharman BS. Effect of vitamin A on keratinization intherat. Am.Ass.Adv.Sci., 114-131, (1962).
• Mellanby EA: A Story of Nutritional Research. Baltimore, 74,(1950).
• Hayes KC, Cousins RJ.: Vitamin A Defıciency and Bone Growth. 130-132,(1970).
• Thaompson JN: Vitamin A in development of the embrio. Am.
• Langman J, Welsch GW.: Excess Vitamin A and development of the cerebral cortex. J. Comp. Neurol., 131, 15, (1967).
• Niazi IA, Saxena S.: Relationship between inhibiting influence of vitamin A and developmental stage of regeneration ta.il in toad tadpoles. I.J.Exp.Biol.,17, 866-868, (1979).
• Thompson J.: Problems of offical methods and new techniques for analysis of foods and feeds for vitamin A 8, (1986).
• Carr PH and Price E.: Colour reactions attributed to vitamin A Biochem. J., 20, 497, (1926).
• Dugan R., Frigerio N. and Siebert J.: Colorimetric determinatıon of vitamin A and it’s derivatives with trifloroaceticacide. Anal. Chem., 36, 114,(1964).
• Bayfıeld RF.: Colorimetric determination of vitamin A with trichloroaceticacide. Anal. Chem., 39, 282, (1971).
• Caldwell MJ, Parrish DB and Schrenk W.: The response of different photometers to the color produced by vitamin A and carotene with antimony trichloride. Trans, Cans. Acad. Sci., 49, (197, (1946).
• Sobel A and Werbin H.: Activated glycerol dichlorohydrin. A new colorimetric reagent for vitamin A Ind. Eng. Chem. Anal. Ed., (1946).
• Sobel A and Snow S.: The estimation of serum vitamin A with activated glycerol dichlorohydrin. J. Biol. Chem., (1947).
• Bayer J., Toro G. and Ackerman P.: Bray’s Clinical Laboratory Methods. (1962).
• Brochmann H. and Schotter H.: Alumina oxides with graded adsorbtion povverful chromotgraphic adsorbtion. Chem. Ber., 73-74, (1941).
• Anonymous.: Manuel for nutrition surveys. Second edition. National Instutes of Health. Bethesda, Md., (1963).
• William H.: Offical Methods of Analysis of the Association of Official and Analytical Chemists. (1975).
• Wiggins RA: Proc. Analyt. Div. Chem. Soc., (1976).
• Bell JG.: Chem and Industry. (1971).
• Çamaş H.: Süt ineklerinde kan plazmasında ve sütte karotin, vitamin A ve bazı yağ asitleri yönünden araştırmalar. ( Doçentlik tezi ), (1979).
• Thompson J., Duval S. and verdier B.: Investigation of carotenoids in human blood using HPLC. J. Micronut. Analysis, 1, 81-91, (1985).
• Coverly S.: Automated determination of vitamin A in milk. J. Micronut. Analysis, 1,65-74, (1985).
• Murti T., Jacob G. and Delthara V.: Detemination of vitamin A in infalt milk powders by HPLC. India J. Dairy Sci., 2, 42, (1989).