Sıçanlarda implantasyonda endometriyum dokusunun hücresel ve sıvısal savunma sistemi hücreleri üzerinde histokimyasal ve histometrik araştırmalar I. Hücresel savunma sistemi hücreleri

Yıl 2000, Cilt: 6 Sayı: 1-2 - 2000, 122 - 130, 18.06.2000

Ahmet Koç Mehmet Kanter

Öz

Bu çalışma, implantasyonda sıçan endometriyum dokusunun hücresel savunma sistemi hücrelerinin dağılımları ve yoğunluklarının histokimyasal ve histometrik yöntemlerle belirlenmesi amacıyla yapıldı. Çalışmada, 42 adet dişi Wistar Albino sıçan kullanıldı. Sıçanlardan alman uteruslardan hazırlanan kriyostat kesitlere, alfa naftil asetat esteraz pozitif hücreleri belirlemek için ANAE enzim boyaması yapıldı. T-lenfositlerin ve uterus doğal öldürücü hücrelerinin uNK’ların , implantasyonun ilk üç gününde desidual alan içinde gittikçe arttığı: endometriyumda ise, desidual alana göre daha az olan bu hücrelerin sayısının üçüncü günde desiduadaki hücre sayısına yakın bir oranda artış gösterdiği gözlendi. İmplantasyonun dördüncü gününden itibaren endometriyumda azalmaya başlayan bu hücrelerin sayılarında, altıncı günde yeniden şekillenen endometriyumla birlikte bir artışın olduğu görüldü. İmplantasyonda endometriyumda bulunmayan makrofajların miyometriyumda oldukça fazla sayıda olduğu tespit edildi. Bu çalışmada, endometriyum dokusunun hücresel savunma sistemi hücrelerinin implantasyonun farklı günlerine göre değişik dağılımlar ve yoğunluklar gösterdiği tespit edildi. Maternal immun tepkinin baskılanarak fötus'un canlılığını sürdürmesinde, diğer bazı faktörlerin genetik ve hormonal yanı sıra implantasyon sürecinde endometriyum dokusunun savunma sistemi hücrelerinin dağılımlarında meydana gelen değişimlerinde etkili olabileceği sonucuna varıldı

Anahtar Kelimeler

Sıçan, Endometriyum, împlantasyon, Hücresel savunma hücreleri

Investigation of the cellular and humoral immune system cells in the endometrium tissue of the rat with histochemical staining and histometric methods during implantation I. Cellular immune system cells

Öz

This study was performed to investigate the distribution and density of the cellular immune system cells in the endometrium tissue of the rat with histochemical staining and histometric methods during implantation. Foıty-two female Wistar albino rats were used. The sections cut using a cryostat microtome were stained with the Acid a- Napthyl Acetate Esterase in order to observe ANAE positive cells. The number of lymphocytes and uterine natuıal killer cells increased in desidual area during the fırst three days of the implantation. The number of these cells in endometrium was less than that in desidual area but, they became equal at the end of third day of implantation. The number of these cells appeared less on the fourth day of implantation but with the growth of endometrium it increased again by sixth day of implantation. Macrophages that were not found in endometrium at implantation were abundantly deteeted in myometrium. In this study, the distribution and density of the cellular immune system cells in the endometrium tissue during implantation were determined. It was concluded that in the suppression of the maternal immune response to allow fetus survive, the changes in the distribution of immune system cells in endometrium during implantation may be important besides other factors such as genetically and hormonal determinants

Anahtar Kelimeler

Rat, Endometrium, implantation, Cellular defence celi

Kaynakça

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