SERUM SIALIC ACID, LİPİD-BOUND SIALIC ACID LEVELS IN SHEEP NATURALLY CHRONIC INFECTED WITH FASCIOLA HEPATİCA

Yıl 2006, Cilt: 9 Sayı: 2, 40 - 46, 01.06.2006

Handan Mert Süleyman Kozat Suat Ekîn Nihat Mert İbrahim Yörük

Öz

The aim of this study to show the importance of sialic acid and lipid-bound sialic acid levels besides some hematological and biochemical parameters with respect to prognosis in detecting the degeneration and functional disorders in the livers of sheep with chronic fascioliasis. This study was carried out on twenty-two female Akkaraman sheep consisting of 10 healthy sheep and 12 sheep naturally chronic infectedwith Fasciola hepatica. In the statistical analyses, in the beginning of the study day 0. , levels of sheep with chronic fascioliasis were compared with the control group levels, a signifîcant decrease was observed in the Hct p

Anahtar Kelimeler

Sheep, Fasciola hepatica, sialic acid, lipid-bound sialic acid

DOĞAL KRONİK FASCİOLA HEPATICA'IA KOYUNLARDA SERUM SIALIK ASİT SERUM LİPİD-BAĞLI SIALIK ASİT DÜZEYLERİ

Öz

Kronik Fasciolazis'li koyunlarda karaciğerde meydana gelen dejenerasyon ve fonksiyonel bozuklukların tespitinde prognoz bakımından hematolojik ve bazı serum biyokimyasal parametrelerinin yanında sialic asit ve lipid-bağlı sialik asit düzeylerinin önemini ortaya koymak için yapıldı. Çalışmada 12'si kronik doğal Fasciola hepatica’ lı ve 10 sağlıklı olmak üzere toplam 22 koyun kullanıldı. Yapılan istatistiksel analizlerde, çalışmanın başlamcm O.günde kronik Fasciolazis'li koyunların değerleri kontrol grubun değerleri karşılaştırıldığında Hct p

Anahtar Kelimeler

Koyun, Fasciola hepatica, sialik asit, lipid-bound sialik asit

Kaynakça

• 1. Anderson PH, BerrettS, BrushPJ,Hebert CN, Parfıtt JW, Patterson DS:. Biochemical indicators of liver injury in calves with experimental fascioliasis. Vet Rec. 15:43- 45,(1977).
• 2. Sykes AR, Coop RL, Robinson MG: Chronic şubelin ical ovine fascioliasis: plasma glutamate dehydrogenase, gamma-glutamyl transpeptidase and aspartate aminotransferase activities and their significance as diagnosticaids. ResVetSci 28:71-75(1980).
• 3. Bhavanandan VP, Sheykhnazari M: Adaptation of the periodateresorcinol method for detennination of sialic acids to a mieroassay using mierotiter plate reader. Anal Biochem. 213: 438-440(1993).
• 4. Schauer R: Achievements and challenges of sialic acid researeh. J. Glycoconj. 17:485-499,(2000).
• 5. Tewarson SL, Mittal VP, Singh M. Gupta GP: Serum sialic acid- an important cancer marker. J. Indian Cancer. 30:125131,(1993).
• 6. Romppanen J: Serum Sialic Acid in Clinical Diagnostics, Kuopio Unyversity Publications D. Medical Sciences 309, Doctoral dissertation (2003).
• 7. Shamberger RJ: Serum sialic acid in normals and in cancer patients. J Clin Chem Clin Biochem. 22: 647-651, (1984).
• 8. Romppanen J, Petâjâ J, Heikinheimo M, Monoııen I: Total and lipid-bound serum sialic acid in children with malignaney or infeetions. Anticancer Res. 18: 27932798,(1998).
• 9. O’Kennedy R, Bems G, Moran E, Smyth H, Carroll K, Thomes RD, O’Brien A, Fennelly J, Butler M: A critical analysis of the use of sialic acid determination in the diagnosis of malignaney. Cancer Lett. 58:91- 100,(1991).
• 10. Seider A, Graf N, Sitzmann F: Wertigkeit der Sialinsâurebestimmung im Serum bei Kindem, PâdiatrPadol. 27: 4346,(1992).
• 11. Doğan H, Pasaoglu H, Ekinciler OF, Tatlisen N : A comparative study of total protein, total and lipidassociated serum sialic acid levels in patients with Behcet’s disease and control groups. Açta ophthalmol Copenh. 70: 790794, (1992).
• 12. Allain P, Oliver E, Le-Bouil A, Benoit C, Geslin P, Tadei A: Increase of sialic acid concentration in the plasma ofpatients withcoronary disease. Presse Med 25:96- 98,(1996).
• 13. Vural P, Akgul C, Canbaz M: Alterations of total and lipid-bound sialic acid levels inrecurrent abortion. Int J Fertil Womens Med. 46:315-319, (2001).
• 14. Boch J, Supperer R: Veterinârmedizinische Parasitologie. 3. AufL Paul Parey Verlag, Berlin und Hamburg. (1983).
• 15. Happic FA, Boray JC: Quantitative diagnosis of chronic fasciolosis. 1. Comparative studies on quantitative feacel examinations for chronic Fasciola hepatica infectioninsheep. AustVet J 45: 326-338, (1969).
• 16. Oosthuizen WTJ, Erasmus JB, Boelema E, Grove JT: Efficacy of moxidectin against internal parasites of sheep. JSAffVetAss 64: 28-30.(1993).
• 17. Dedie K, Bostedt H: Schafkrankheiten. Ulmer Verlag, Stuttgart(1985).
• 18. Zimmerman GL: Liver flukes in ruminants. In Large animal internal medicine. Ed. Smith, B. Mosby Publ., St. louis, Baltimore, Philadelphia, Toronto. (1989).
• 19. Jain NC: The deer. In: Schalm's Veterinary Haematology, 5th ed., Shalm, O.W., Jain, N.C., Carol, E.J. Eds., Lea and Febiger, Philadelphia. (1986).
• 20. Katopodis N, Hirshaut Y, Geller NL, Stock CC: Lipid associated sialic acid test for the deteetion of human cancer. CancerRes 42: 5270-5275,(1982).
• 21. Sydow GA: Simplifîed quick method for determination of sialic acid in serum. Biomed. Biochem. Açta. 44: 1721- 1723, (1985).
• 22. Spithill TW, Dalton JP: Progress in development of liver fluke vaccines.ParasitolToday. 14: 224228,(1998).
• 23. Dalton JP: Epidemiology and control. In: Dalton, J.P.: Fasciolosis. CABI Publishing, Cambridge, pp 113- 149.(1999).
• 24. Marques SMT, Scrofemeker ML: Fasciola hepatica infeetion in cattle and buffaloes in the State of Rio Grande do Sul, Brazil. Parasitol. Latinoam. 58: 169- 172,(2003).
• 25. Benchaoui HA, McKellar QA: Effect of early treatment with rafoxanide on antipyrine clearance in sheep infected with Fasciola hepatica. Xenobiotica. 23: 439448,(1993).
• 26. Ferre I, Lopez P, Rojo-Vazquez FA, Gonzalez-Gallego J: Experimental ovine fasciolosis: antipyrine clearance as indicator of liver damage. Vet. Parasitol 62:93-100, (1996).
• 27. Galtier P, Larrieu G, Tufenkji AE, Franc M: Influence of experimental fascioliasis on the activity of drugmetabolizing enzvmes in lamb liver. Drug Metab Dispos 14:137141. (1986)
• 28. Ferre I, Lopez P, Gonzalo-Orden M, Julian MD, Rojo FA, Gonza' lez-Gallego J: The effects of subclinical fascioliasis on hepatic secretory funetion in sheep. ParasitolRe 81:127131, (1995).
• 29. Kouider S, Kolb E: Der Gehalt an Ascorbinsaure, an glucose, an gesampprotein, an AL AT und an ASAT im blutplasma von gesunden sehafen sowie bei solehen mit parasitenbefall vor und nach i.v. injektion von ascorbinsaurelosung. Monatshefte-für- Veterinarmedizin. 49: 135-141,(1994).
• 30. Bain PJ: Liver. In: Latimer KS, Mahaffey EA, Prasse KW, Duncan and Prasse's Veterinary Laboratory Medicine: Clinical Pathology, 4th ed. Ames, Iowa State Press, 193-214,(2003),
• 31. Stockham SL, Scott MA: Fundamentals of Veterinary Clinical Pathology. Ames, Iowa State Press, 43 8: 446- 450.2002
• 32. TimoteoOV, Maco JV, V. Maco PJ, NeyraGY, Leguı'a JR: Espinoza Characterization of the humoral immune response in alpacas (Lama pacos) experimentally infected with Fasciola hepatica against eysteine proteinases Fasl and Fas2 and histopathological findings. Veterinary Immunology and Immunopathology 106: 7786.(2005).
• 33. Mitruka B M, Rawnsley HM: Clinical biochemical and hematological reference values in normal experimental animals and normal humans. 2nd Ed., Year Book Medical Publishers Inc., Chicago, (1981).
• 34. Chauvin A, Bouvet G, Boulard C : Humoral and cellular immune responses to Fasciola hepatica experimental primary and secondary infection in sheep. Int J Parasitol.25:12271241,(1995).
• 35. Conboy GA, Stromberg BE: Hematology and clinical pathology of experimental Fascioloides magna infection in cattle and guinea pigs. Veterinary Parasitology. 40:241-255(1991).
• 36. Hawkins CD: The use of hemoglobin, packed-cell volüme and serum sorbitol dehydrogenase as indicators of the development of fascioliasis in sheep. Vet Parasitol 15:125-133, (1984).
• 37. Presidente PJ, McCraw BM, Lumsden JH: Experimentally induced Fasciola hepatica infection in white-tailed deer. I. Clinicopathological and parasitological features, Can J Comp Med. 39: 155- 165,(1975).
• 38. Crook MA, Haq M, Tutt P: Serum sialic acid and its relationship to various haematological parameters, including erythrocyte sedimentatıon rate. Br J Biomed Sci. 54:100-103, (1997).
• 39. Blood DC, Radostits DM, Henderson JA: Veterinary Medicine, 7th Ed., Bailiere Tindall, London. (1987).
• 40. Degheidy NS, Amin MM, Ghazy AA: Effect of fasciolosis on liver function before and after treatment in sheep. Egyptian Journal of Comparative Pathology and Clinical Pathology. 1: 65-76, (1990).
• 41. Swarup D, Upadhyay DS, Pachauri SP: Some biochemical indices in naturally occurring fascioliasis ingoats. Res VetSci 40:276-277, (1986).
• 42. Kokoglu E, Sönmez H, Uslu E, Uslu I: Sialic acid levels in various types of cancer, Cancer Biochem Biophys. 13:57-64, (1992).
• 43. Polivkova J, Vosmikova K, Horak L : Utilization of detemıining lipid-bound sialic acid for the diagnosis and furtherprognosis ofcancer. Neoplasma. 39:233- 236,(1992).
• 44. Pönniö M, Alho H, Heinalâ P, Nikkari ST, Sillanaukee P: Serum and saliva levels of sialic acid are elevated in alcoholics. Alcohol Clin. Exp. Res. 23: 10601064, (1999).
• 45. Lindberg G, Eklund GA, Gullberg B, Rastam L: Serum sialic acid concentration and cardiovascular mortality. JBiolMed302:143-146,(1991).
• 46. Crook MA, Tutt P, Pickup JC: Elevated serum sialic acid concentration in NIDDM and its relationship to blood pressure and retinopathy. Diabetes Çare. 16: 57-60, (1993).
• 47. Kongtawelert P, Tangkijvanich P, Ong-Chai S, Poovorawan Y: Role of serum total sialic acid in differentiating cholangiocarcinoma from hepatocellular carcinoma. J World Gastroenterol 9: 2178-2181,(2003).
• 48. VanDijkW, PosO, Van der Stelt ME, Moshage HJ, Yap SH, Dente L, Baumann P, Eap CB: Inflammationinduced changes in expression and glycosylation of genetic variants of alpha 1-acidglycoprotein. Studies with human sera, primary cultures of human hepatocytes and transgenic mice. J Biochem 276: 343-347,(1991).
• 49. Crook MA, Treloar A, Haq M, Tutt P: Serum total sialic acid and acute phase proteins in elderly subjects. Eur J Clin Chem Clin Biochem. 32:745-747, (1994).
• 50. Taniuchi K, Chifu K, Hayashi NA: New enzymatic method for the determination of sialic acid in serum and its application for a marker of acute phase reactants. J KobeMed.Sci.27:91-102,(1981).
• 51. Murray RK, Granner DK, May es PA, Rodwell VW: Lipid transport and storage. In: Harper’s biochemistry, 23rd ed. Norwalk, Conn: Appleton & Lange. 250-258, (1993).
• 52. Schutter EMJ, Visser JJ, Van Kamp GJ, MensdorffPouilly S, van Dijk W, Hilgers J, Kenemans P: The utility of lipid-associated sialic acid (LASA or LSA) as a serum marker for malignancy. A review of the literatüre. TumourBiol. 13:121-132,(1992).
• 53. Vardi J, Tadros GH, Foemmel R, Shebes M: Plasma lipidassociated sialic acid and serum CA 125 as indicators of disease status with advanced ovarian cancer. ObstetGynecol., 74:379-383, (1989).
• 54. Ropka ME, Goodwin WJ, Levine PA, Sasaki CT, Kirchner JC, Cantrell RW: Effective head and neck tumor markers. The continuing quest. Arch Otolaryngol Head Neck Surg. 117:101114,(1991).