Yield and Quality Characteristics of Lettuce Lines Developed by Mutation Breeding
Abstract
In this study, we aimed to create genetic variation through physical mutagen application to lettuce (Lactuca sativa var. longifolia), a vegetable species whose leaves are consumed fresh, to obtain new varieties rich in nutritional content that can create market demand from this gene pool. Cervantes and Escule lettuce seeds were irradiated with effective mutation doses determined specific to the variety using a Cobalt-60 gamma ray source. Selection and inbreeding studies were performed for 4 generations, and 36 mutant lines were selected from the lines at the M4 stage to be used in subsequent experiments. Height, diameter, weight, leaf color, water-soluble dry matter (WSDM), chlorophyll, total carotenoid, vitamin C, lutein and total phenolic content of lettuce heads were examined in 36 mutant lines selected and in 4 commercial control varieties. The differences between the data obtained were statistically significant. Mutant lines numbered 62, 66, 71, 72, 74, 77, 84, and 100 which were found to be superior in terms of both morphological characteristics and nutritional content, were selected for inclusion in yield trials as a variety of candidates. These findings represent a significant step in increasing genetic diversity in agricultural production and developing lettuce varieties with higher yield and nutritional value.
Keywords
Supporting Institution
This research was funded by the Republic of Turkey Ministry of Agriculture and Forestry, General Directorate of Agricultural Research And Policies.
Ethical Statement
Ethical approval is not required for this study because no harm was done to nature and the environment.
References
- Aeamovie-Djokovie, G., Pavlovie, R., Mladenovie, J., & Djurie, M. (2011). Vitamin C content of different types of lettuce varieties. Acta Agriculturae, 32, 83-89.
- Afton, W. D. (2018). Evaluation of growth characteristics, yield, marketability, and nitrate levels of lettuce (Lactuca sativa L.) cultivars produced in South Louisiana. (Master’s thesis), Louisiana State University, Baton Rouge, United States.
- Anne, S., & Lim, J. H. (2020). Mutation breeding using gamma irradiation in the development of ornamental plants: A review. Flower Research Journal, 28(3), 102-115. doi:https://doi.org/ 10.11623/frj.2020.28.3.01.
- Anusiya, M., & Sivachandiran, S. (2019). Effect of different shade levels on growth and yield performance of lettuce. International Journal of Forestry and Horticulture, 5(3), 1-4. doi:https://doi.org/10.20431/2454-9487.0503001.
- Atak, E., & Uslu, E. (2018). Phenolic compounds, extraction methods and analytical techniques. Journal of Technical Sciences, 3(27), 39-48.
- Bado, S., Forster, B. P., & Nielen, S. (2015). Plant mutation breeding: Current progress and future assessment. Plant Breeding Reviews, 39, 23-88. doi:https://doi.org/10.1002/978111910 7743.ch2.
- Baslam, M., Morales, F., Garmendiac, I., & Goicoecheaa, N. (2013). Nutritional quality of outer and inner leaves of green and red pigmented lettuces (Lactuca sativa L.) consumed as salads. Scientia Horticulturae, 151, 103-111. doi:https://doi.org/10.1016/j.scienta.2012.12.023.
- Beşirli, G., Göçmen, M., Yanmaz, R., & Kantoğlu, K. Y. (2008). Diversity of some garlic (Allium sativum L.) genotypes and mutants by RAPD markers. In G. Çağlar, K. Abak, & I. E. Akinci (Eds.), Proceedings of 4th National Vegetable Culture Symposium Book (pp. 49-54).
Details
Primary Language
English
Subjects
Crop and Pasture Breeding
Journal Section
Research Article
Authors
Şule Kökpınar
*
0000-0002-6481-5618
Türkiye
Publication Date
December 25, 2025
Submission Date
March 27, 2025
Acceptance Date
July 1, 2025
Published in Issue
Year 1970 Volume: 35 Number: 4
