Determination of Structural, Conformational and Antiradical Properties of Carvacrol Encapsulated with Yeast Cells (Saccharomyces cerevisiae)

Abstract

Carvacrol is the most studied phenolic substance, one of the essential components of thyme. The encapsulation technique can be used to make more stable carvacrol, which has decreased stability due to environmental factors. In this study, it was aimed to encapsulate and characterize carvacrol, which is used in the food, agriculture and pharmaceutical industry, with yeast cells. Yeast cells were pretreated with plasmolysis and carvacrol was encapsulated with both plasmolysed and non-plasmolysed cells. Carvacrol retained was 80.79% in the plasmolysed yeast cells and it was 90.43% in the non-plasmolysed yeast cell. The total amount of phenolic substances in the capsules was determined as to be 138.1 and 146.8 mg GAE/g for plasmolysed and non-plasmolysed yeast cells capsules respectively and the non-plasmolysed yeast capsules exhibited higher antiradical activity compared to the plasmolysed encapsules (PE). In addition, the encapsulation of carvacrol by yeast cells was proved by FTIR technique and structural properties were determined by scanning electron microscope.

Keywords

• Antiradical,Carvacrol,Yeast cell,Plasmolysis,S. cerevisiae

Maya Hücreleri (Saccharomyces cerevisiae) ile Enkapsüle Edilen Karvakrolün Yapısal, Konformasyonel ve Antiradikal Özelliklerinin Belirlenmesi

Öz

Karvakrol, kekik bitkisinin uçucu bileşenlerinden en çok çalışılan fenolik yapılı bir maddedir. Çevresel faktörlere bağlı olarak stabilitesi azalan karvakrolü daha stabil bir ürün haline dönüştürmek için enkapsülasyon tekniğinden faydalanılabilmektedir. Bu çalışmada gıda, ziraat ve ilaç endüstrisinde kullanımı olan karvakrolün maya hücreleri ile biyo-enkapsüle edilmesi ve karakterizasyonu amaçlanmıştır. Maya hücrelerine ön işlem olarak plazmoliz işlemi uygulanmış ve hem plazmoliz olmuş hem de olmamış hücreler ile karvakrol enkapsüle edilmiştir. Plazmolize olmuş ve olmamış maya hücrelerinde tutulan karvakrol oranları plazmolize olmuş maya hücresinde %80.79, plazmolize olmamış maya hücresinde ise %90.43 olarak tespit edilmiştir. Enkapsüllerin toplam fenolik madde miktarları plazmolize olmuş ve olmamış hücre enkapsülleri için sırasıyla 138.1 ve 146.8 mg GAE/g olarak belirlenmiş ve maya enkapsülleri içerisinde plazmoliz olmamış enkapsül (POE), plazmolize enkapsüle (PE) kıyasla daha yüksek antiradikal aktivite sergilemiştir. Ayrıca gerçekleştirilen FTIR tekniği ile karvakrolün maya hücreleri tarafından enkapsülasyonu doğrulanmış ve taramalı elektron mikroskobu ile yapısal özellikleri belirlenmiştir.

Anahtar Kelimeler

• S.cerevisiae,maya hücresi,karvakrol,plazmoliz,biyoaktivite

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