Patlıcan Meyvelerinde (Solanum melongena L.) Metil Jasmonat Uygulamalarının Enzimatik Kararma ve Antioksidatif Enzimler Üzerine Etkileri

Öz

Meyveleri klimakterik olmayan patlıcanlar, üşüme zararına hassas ve hasat sonrası raf ömrü kısa olduğundan dolayı önemli ekonomik kayıplara neden olmaktadır. Çalışma materyali olan patlıcanlar Türkiye de Van'ın Gevaş ilçesinde hasat edilmiştir. Patlıcan meyveleri olgunluk seviyeleri dikkate alınarak hasat edilmiş ve daha sonra köpük tabaklarda modifiye atmosfer paketleme yapılarak 10 ve 20 °C'de % 90-95 bağıl nem içeren soğuk hava depolarında 21 gün boyunca depolanmıştır. Aynı olgunluğa sahip meyveler 3 ayrı gruba ayrılmıştır. Birinci grup meyveler kontrol olarak saf suya daldırılmıştır. İkinci grup meyveler 1 µM Metil Jasmonat (MeJA) çözeltisine 10 dakika süreyle daldırılmıştır. Üçüncü grup meyvelere ise 5 µM Metil Jasmonat (MeJA) çözeltisine 10 dakika süreyle daldırılmıştır. Hasat sonrası uygulanan Metil Jasmonat'ın solunum hızı, Süperoksit dismutaz (SOD), katalaz (CAT), polifenol oksidaz (PPO) ve malondialdehit (MDA) üzerine olan etkisi araştırılmıştır. Çalışmadan elde edilen sonuçlara göre, 20 °C'de depolanan meyvelerde 1 uM Metil Jasmonat uygulamasının, solunum hızı, SOD, PPO ve MDA gibi parametreler açısından en iyi sonuçları verdiği tespit edilirken, 5 uM Metil Jasmonatın ise CAT enzim aktivitesi açısından en iyi sonucu verdiğini göstermiştir. Sonuç olarak, MeJA uygulamalarının depolama periyodu boyunca antioksidatif enzimler ve solunum hızı üzerine etkisi olduğu söylenebilir.

Anahtar Kelimeler

• Antioksidatif enzimler,Solunum hızı,Depolama,MAP

Effect of Methyl Jasmonate on Enzymatic Browning and Antioxidant Enzyme System of Eggplant Fruit (Solanum melongena L.)**

Abstract

Eggplant fruit result in significant economic losses, as being non-climacteric is sensitive to chilling injury and short postharvest life. Eggplants were harvested in Gevas district of Van, Turkey. The eggplant fruits were harvested by taking maturity levels into consideration and then placed in foam plates and covered with Modified Atmosphere Packaging (MAP) for 21 days in cold air depots containing 10 and 20 °C temperature and 90-95% relative humidity. The fruits of the same size were divided into 3 different groups. The first group of fruits was immersed in distilled water as a control. The second group of fruits were immersed in 1 μM MeJA solution for 10 minutes. The third group was immersed in 5 μM MeJA solution for 10 minutes. The effect of postharvest Methyl Jasmonate treatment during the storage period on respiratory rate, Superoxide dismutase (SOD), catalase (CAT), polyphenol oxidase (PPO), and malondialdehyde (MDA) were evaluated. The results obtained from this study suggest that 1 µM Methyl Jasmonate application gives the best results in terms of parameters such as respiratory rate, SOD, PPO, and MDA at 20 °C, while 5 µM Methyl Jasmonate was found to be the most positive one in terms of CAT enzyme activity. As a result, it can be suggested that MeJA treatments were effective on antioxidativ enzymes and respiration rate during the storage period.

Keywords

• Antioxidative enzymes,Respiration rate,Storage,MAP

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