Reactions of Fifteen Onion Cultivars Commonly Cultivated in Turkey to Leek yellow stripe virus (LYSV)

Yıl 2021, , 71 - 79, 30.03.2021

Adyatma Irawan Santosa , Filiz Ertunç

https://doi.org/10.29133/yyutbd.748558

Cited By: 2

Öz

Onion (Allium cepa L.) cultivars commonly cultivated in Turkey were for the first time examined for their reactions to Leek yellow stripe virus (LYSV). Fifteen onion cultivars consisted of red, white, and yellow bulb cultivars were grown in pots and mechanically inoculated with LYSV-12.6Po, a newly characterized Turkish isolate. The experiment was in completely randomized block design with three replicates, containing 30 plants in each. One replicate was also planted as control. Serological and molecular detection methods, and statistical analysis on five growth parameters were used to evaluate their reactions to LYSV. LYSV infections in KG kırmızı, UG beyaz, Şampiyon, Perama, Seyhan, and Hazar were not detected by DAS-ELISA, but RT-PCR gave positive results to LYSV for all 15 tested cultivars. t-test results showed that LYSV inoculation caused significant reduction in all growth parameters for most cultivars. Duncan’s multiple range test found that Anka, UG kahverengi, and KG kahverengi performed badly in all growth parameters. On the other hand, Şampiyon and Perama were not significantly different to each other in all growth parameters, and performed very well in plant weight, plant height, and no. of leaves parameters. Based on results of this study, Şampiyon and Perama were considered as the most tolerant cultivar to LYSV.

Anahtar Kelimeler

onion, LYSV, cultivar reactions

Teşekkür

Authors are thankful to Arya Widyawan, M.Sc. from Plant Protection Department, King Saud University, Saudi Arabia for his help in statistical calculations.

Türkiye’de Yaygın Olarak Üretilen On Beş Soğan Çeşidinin Leek yellow stripe virus (LYSV)’üne Karşı Reaksiyonları

Öz

Türkiye’de yaygın olarak yetiştirilen soğan (Allium cepa L.) türlerinin bu çalışma ile Leek yellow stripe virus (LYSV)’üne karşı reaksiyonları ilk olarak araştırılmıştır. Kırmızı, beyaz ve sarı soğan çeşitlerinden oluşan 15 tür saksılarda yetiştirilmiş ve mekaniksel olarak Türkiye’de yeni karakterize edilmiş olan LYSV-12.6Po izolatı ile aşılanmıştır. Denemede rastgele tam blok tasarımıyla 3 tekerrür ve her tekerrürde 30 bitki düzenlenmiştir. Ayrıca 1 tekerrür de kontrol olarak hazırlanmıştır. Serolojik ve moleküler testlerle LYSV’nin bitkilerdeki varlığı test edilmiş ayrıca ele alınan 5 gelişme parametrelerinin belirlenmesi için istatistiksel analizler uygulanmıştır. LYSV enfeksiyonu KG kırmızı, UG beyaz, Şampiyon, Perama, Seyhan ve Hazar çeşitlerinde DAS-ELISA ile belirlenememiştir, ancak RT-PCR testlerinde ele alınan 15 çeşidin tümü de pozitif sonuçlar vermiştir. t-testi sonucuna göre LYSV enfeksiyonu, ele alınan parametrelerde çeşitlerin çoğunda belirgin azalmalara neden olmuştur. Duncan çoklu aralık testine göre, Anka, UG kahverengi ve KG kahverengi tüm gelişme parametreleri açısından çok kötü sonuçlar vermişlerdir. Diğer taraftan Şampiyon ve Perama ele alınan tüm gelişme parametreleri açısından birbirinden çok farklı olmayıp, bitki ağırlığı, bitki boyu ve yaprak sayısı açısından en iyi sonuçları vermişlerdir. Bu çalışmadan elde edilen sonuçlara göre Şampiyon ve Perama, LYSV enfeksiyonuna en tolerans çeşitler olarak belirlenmişlerdir.

Anahtar Kelimeler

soğan, LYSV, çeşit reaksiyonları

Kaynakça

• Adams, M. J., Antoniw, J. F., & Beaudoin, F. (2005). Overview and analysis of the polyprotein cleavage sites in the family Potyviridae. Mol. Plant Pathol., 6, 471-487. doi.org/10.1111/j.1364-3703.2005.00296.x
• Al-Shahwan, I. M., Santosa, A. I., Abdalla, O. A., & Al-Saleh, M. A. (2018). Behavior of watermelon cultivars after inoculation with Watermelon mosaic virus in Saudi Arabia. Journal of Agricultural Science and Technology A, 8, 306-314. doi.org/10.17265/2161-6256/2018.05.006
• Azad, M. A. K., Amin, L., & Sidik, N. M. (2014). Gene technology for Papaya ringspot virus disease management. The Scientific World Journal, 2014, 1-11. doi.org/10.1155/2014/768038
• Brunt, A. A., Crabtree, K., Dallwitz, M. J., Gibbs, A. J., & Watson, L. (1996). Plant viruses online: Description and lists from the VIDE database. Leek yellow stripe potyvirus. http://bio-mirror.im.ac.cn/mirrors/pvo/vide/descr437.htm. Accessed date: 02.05.2020.
• Collard, B. C. Y., & Mackill, D. J. (2008). Marker-assisted selection: an approach for precision plant breeding in the twenty-first century. Phil. Trans. R. Soc. B, 63(1491), 557-572. doi:10.1098/rstb.2007.2170
• Dovas, C. I., Hatzibukas, E., Salomon, R., Barg, E., Shiboleth, Y. M., & Katis, N. I. (2001). Comparison of methods for virus detection in Allium species. J Phytopathol, 149, 731-737. doi.org/10.1046/j.1439-0434.2001.00705.x
• Dovas, C. I., & Vovlas, C. (2003). Viruses infecting Allium spp. in Southern Italy. Journal of Plant Pathology, 85(2), 135.
• Elnagar, S., El-Sheikh, M. A., & Abd El-Wahab, A. S. E. (2011). Effect of natural infection with Onion yellow dwarf virus (OYDV) on yield of onion and garlic crops in Egypt. Journal of Life Sciences, 5, 634-638.
• Fajardo, T. V. M., Nishijima, M., Buso, J. A., Torres, A. C., Ávila, A. C., & Resende, R. O. (2001). Garlic viral complex: identification of Potyviruses and Carlavirus in central Brazil. Fitopatologia Brasileira, 26, 619-626.
• Fidan, H. (2010). Sarımsak, soğan ve pırasadaki virüs hastalıklarını saptanması ve Taşköprü 56 sarımsak tipinin en yaygın virüse karşı reaksiyonunun belirlenmesi (in Turkish). (PhD), Çukurova University, Adana, Turkey.
• Fidan, H., & Baloglu, S. (2009). First report of Onion yellow dwarf virus and Leek yellow stripe virus in garlic in Turkey. Plant Dis., 93(6), 672. doi.org/10.1094/PDIS-93-6-0672C
• Gonsalves, D., Tripathi, S., Carr, J. B., & Suzuki, J. Y. (2010). Papaya ringspot virus. The Plant Health Instructor. doi.org/10.1094/PHI-I-2010-1004-01
• Klukáčková, J., Navrátil, M., & Duchoslav, M. (2007). Natural infection of garlic (Allium sativum L.) by viruses in the Czech Republic. J Plant Dis Protect, 144(3), 97-100. doi.org/10.1007/BF03356714
• Korkmaz, S, & Cevik, B. (2009). Leek yellow stripe virus newly reported in Turkey. Plant Pathol., 58, 787. doi.org/10.1111/j.1365-3059.2009.02049.x
• Lot, H., Chovelon, V., Souche, S., & Delecolle, B. (1998). Effects of onion yellow dwarf and leek yellow stripe viruses on symptomatology and yield loss of three French garlic cultivars. Plant Dis., 82, 1381-1385. doi.org/10.1094/PDIS.1998.82.12.1381
• Lot, H., Chovelon, V., Souche, S., Delecolle, B., Etoh, T., & Messiaen, C. M. (2001). Resistance to Onion yellow dwarf virus and Leek yellow stripe virus found in a fertile garlic clone. Acta Horticulturae, 555, 243-246. doi.org/10.17660/ActaHortic.2001.555.36
• Lunello, P., Di Rienzo, J., & Conci, V. C. (2007). Yield loss in garlic caused by Leek yellow stripe virus Argentinean isolate. Plant Dis., 91, 153-158. doi.org/10.1094/PDIS-91-2-0153
• Lunello, P., Ducasse, D. A., Helguera, M., Nome, S. F., & Conci, V. C. (2002). An Argentinean isolate of Leek yellow stripe virus from leek can be transmitted to garlic. Journal of Plant Pathology, 84(1), 11-17. dx.doi.org/10.4454/jpp.v84i1.1082
• Paludan, N. (1980). Virus attack an leek: Survey, diagnosis, tolerance of varieties and winter hardiness. Institut for Plantepatologi, 1518, 371-385.
• Presting, G. G., Smith, O. P., & Brown, C. R. (1995). Resistance to Potato leafroll virus in potato plants transformed with the coat protein gene or with vector control constructs. Phytopathology, 85, 436-442. doi.org/10.1094/Phyto-85-436
• Santosa, A. I., & Ertunç, F. (2020). Serological and molecular detection of Leek yellow stripe virus infecting onion (Allium cepa L.) and leek (Allium ampeloprasum L.) in Ankara, Turkey. Bitki Koruma Bülteni, 60(1), 49-56. doi.org/10.16955/bitkorb.622150
• Sevik, M. A., & Akcura, C. (2013). Viruses occurring in onion crop in Amasya province, the major onion producing region in Turkey. Indian Journal of Virology, 24(1), 78-81. dx.doi.org/10.1007%2Fs13337-012-0114-z
• Shahraeen, N., Lesemann, D. E., & Ghotbi, T. (2008). Survey for viruses infecting onion, garlic and leek crops in Iran. Bulletin OEPP/EPPO Bulletin, 38, 131-135. doi.org/10.1111/j.1365-2338.2008.01198.x
• Sharma, P., Sahu, A. K., Verma, R. K., Mishra, R., Choudhary, D. K., & Gaur, R. K. (2013). Current status of Potyvirus in India. Arch Phytopathology Plant Protect, 47(8), 906-918. dx.doi.org/10.1080/03235408.2013.825963
• Tuzlalı, H. T. (2018). Güney Marmara Bölgesi’nde Allium cinsi bitkilerde Potyvirüslerin tanılanmasi ve karakterizasyonu (in Turkish). (PhD), Çanakkale Onsekiz Mart University, Graduate School of Natural and Applied Sciences, Çanakkale, Turkey.
• TÜIK (Türkiye İstatistik Kurumu/Turkish Statistical Institute). (2020). http://www.tuik.gov.tr/Start.do Accessed date: 11.05.2020.
• Vučurović, I., Nikolić, D., Radović, N., Vučurović, A., Ristić, D., Krstić, B., & Stanković, I. (2017). Incidence and distribution of Leek yellow stripe virus in Allium crops in Serbia. Pesticides and Phytomedicine (Belgrade), 32(3-4), 145–155. doi.org/10.2298/PIF1704145V
• Vučurović, I., Vučurović, A., Nikolić, D., Bulajić, A., Milošević, D., Krstić, B., & Stanković, I. (2016). First report of Leek yellow stripe virus in leek in Serbia. Plant Dis., 100(1), 230. doi.org/10.1094/PDIS-04-15-0432-PDN
• Ward, L. I., Perez-Egusquiza, Z., Fletcher, J. D., & Clover, G. R. G. (2009). A survey of viral diseases of Allium crops in New Zealand. Australas Plant Pathol., 38, 533-539. doi.org/10.1071/AP09039
• Worrall, E. A., Wamonje, F. O., Mukeshimana, G., Harvey, J. J. W., Carr, J. P., & Mitter, N. (2015). Chapter One - Bean common mosaic virus and Bean common mosaic necrosis virus: Relationships, Biology, and Prospects for Control. Adv Virus Res, 93, 1-46. doi.org/10.1016/bs.aivir.2015.04.002
• Wylie, S. J., Adams, M., Chalam, C., Kreuze, J., Lopez-Moya, J. J., Ohshima, K., Praveen, S., Rabenstein, F., Stenger, D., Wang, A., & Murilo Zerbini, F. (2017). ICTV Virus Taxonomy Profile: Potyviridae. J. Gen. Virol. 98(3), 352–354. doi:10.1099/jgv.0.000740