Fasulye Genomunda 2,4-Diklorofenoksiasetik Asit Genotoksisitesine Karşı Β-Östradiolün Hafifletici Rolü

Yıl 2018, Cilt: 28 Sayı: 1, 1 - 9, 30.03.2018

Mahmut Sinan Taşpınar Burcu Sığmaz Murat Aydın Esra Arslan Güleray Ağar

https://doi.org/10.29133/yyutbd.305274

Cited By: 1

Öz

2,4-Diklorofenoksiasetik asit (2,4-D) tahıl ekin kültüründe Taraxacum officinale ve geniş yapraklı
yabancı otların gelişmesini önlemek için yaygın olarak kullanılan
herbisitlerden biridir. Ancak, 2,4-D'nin, düşük konsantrasyonlarda bitkilerde
genetik hasarın yanı sıra gözlemlenebilir fizyolojik etkilere neden olduğu
bilinmektedir. Uygulanan 2,4-D'ye karşı bitkilerde memeli hormonlarının
etkisini araştıran herhangi bir rapor bulunmamaktadır. Bu nedenle, bu çalışma
2,4-D'ye maruz bırakılan fasulye (Phaseolus
vulgaris)'deki DNA hasarı, DNA metilasyonu ve DNA stabilitesindeki
değişimleri araştırmayı ve β-östradiolün herhangi bir etkisinin olup olmadığını
belirlemeyi amaçlamıştır. DNA hasar düzeylerini ve DNA metilasyon modelindeki
değişiklikleri tanımlamak için RAPD (Rastgele Çoğaltılan Polimorfik DNA) ve
CRED-RA (Çift Restriksiyon Enzim Kesimi-Rastgele Çoğaltımı) teknikleri
kullanılmıştır. Elde edilen veriler, 2,4-D'nin RAPD profil değişikliklerinde
(DNA hasarında) artışa ve genomik kararlılık stabilitesinde (GTS) ise azalışa
neden olduğunu göstermiştir. 2,4-D'nin neden olduğu etkiler, farklı β-östradiol
konsantrasyonları uygulandıktan sonra azalmıştır. Bu çalışmanın sonuçları,
fasulyelerde 2,4-D herbisitin neden olduğu genetik ve epigenetik
değişikliklerin engellenmesi için β-östradiolün etkin bir şekilde
kullanılabileceğini açıkça göstermektedir.

Anahtar Kelimeler

β-östradiol, DNA metilasyonu, Genotoksisite, DNA hasarı, 2, 4-D

Alleviative Role of Β-Estradiol Against 2,4-Dichlorophenoxyacetic Acid Genotoxicity on Common Bean Genome

Öz

2,4-Dichlorophenoxyacetic acid (2,4-D) is one of the several herbicides that widely
used to prevent development of Taraxacum
officinale and broadleaf weeds on cereal crops culture. However, it is known that
2,4-D can cause genetic damage to plants at low concentrations as well as
produce observable physiological effects. There is no report investigating the
effect of mammalian hormones in crops against the applied 2,4-D. Therefore, the
present study was aimed at investigating levels of DNA damage, changes in DNA
methylation and DNA stability in common bean (Phaseolus vulgaris) exposed
to 2,4-D and determine whether β-estradiol has any effect. RAPDs (Randomly Amplified
Polymorphic DNA) and CRED-RAs (Coupled Restriction Enzyme Digestion-Random
Amplification) techniques were used to define the DNA damage levels and changes in the
pattern of DNA methylation.  The obtained
data demonstrated that 2,4-D led to an increase in RAPDs profile changes (DNA damage), and a
reduction in genomic template stability (GTS). The effects caused by 2,4-D were
decreased after application with different concentrations of β-estradiol. The
results of this study clearly show that β-estradiol could be used function
effectively to prevented from the genetic and epigenetic changes caused by
2,4-D herbicide in common bean.

Anahtar Kelimeler

2 4-D, β-estradiol, DNA methylation, Genotoxicity, DNA damage

Kaynakça

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