Deneysel Olarak Diyabet Oluşturulmuş Sıçanlarda Hba1c, Mda, Gsh-Px Ve Sod Miktarlarının Tayini

Öz

İnsülin sekresyonu veya etkisinde yetmezliği ile karakterize olan ve günümüzde çok yaygın olan Diabetes Mellitus üzerinde yapılan bu çalışmada 7-8 haftalık Wistar cinsi sıçanlar kullanıldı. Rastgele seçilen 10 sıçan kontrol, 20 sıçan diyabetli grup olmak üzere 2 grup oluşturuldu. Diyabetli gruba 45 mg/kg düzeyinde streptozotosin intraperitoneal uygulandı. Tüm hayvanlarda HbA1c, MDA, GSH-Px ve SOD düzeylerine bakıldı. HbA1c diyabetli grupta P<0.001 düzeyinde artış gösterirken, MDA düzeyinde saptanan yükselme istatiksel önem göstermemiştir (P>0.05). GSH-Px düzeyi diyabetli grupta azalmış (P<0.05), SOD miktarı ise diyabetli grupta artmıştır. Sonuç olarak, HbA1c’nin Diabetes Mellitus tanısında kullanılabilecek önemli bir parametre olduğuna ve Diabetes Mellitus’ta antioksidan enzim değişimlerinin hastalığın prognozu açısından önemli olabileceği, klinisyenlerin Diabetes Mellitus’ta bu parametrelere dikkat etmelerinin yerinde olacağı sonucuna varılmıştır.

Anahtar Kelimeler

• Diabetes Mellitus
• Hemoglobin A1c
• Glutatyon peroksidaz
• Malon dialdehit
• Rat
• Süperoksit dismutaz

Investigation on the HbA1c, MDA, GSH-Px and SOD Levels in Experimentally Diabetic Rats

Öz

Diabetes Mellitus is the results of deficiency of insulin secretion and effect was studied on Wistar rats in this research. Rats were randomly divided into 2 groups, 10 rats were healthy control and 20 rats were sick group. To develop experimental diabetes 45 mg/kg streptozotocine was administrated intraperiteonally to 20 rats in sick group. HbA1c, MDA, GSH-Px and SOD analysis were performed to blood samples of all rats. The levels HbA1c were increased significantly P<0.001 in sick group. Increased MDA levels in sick group didn’t show statistically significance (P>0.05). GSH-Px activity was significantly decreased in sick group, SOD activity was increased but statistical significance was not detected (P≤0.05). As conclusion, HbA1c was important parameters for the diagnosis at Diabetes mellitus, antioxidant enzyme activity changes could be important for the prognosis of Diabetes mellitus and clinicians must be careful and take in consideration of these changes during the treatment of their diabetic patient.

Anahtar Kelimeler

• Diabetes Mellitus
• Glutathione Peroxidase
• Hemoglobin A1c
• Malonaldehyde
• Rat
• Superoxide Dismutase

Kaynakça

1. Abou-Seif MA, Youssef AA (2004). Evaulation of some biochemical changes in diabetic patients. Clin Chim Acta, 346 (2), 161-70.
2. Abou-Seif MAM, Youssef H (2001). Oxidative stress and male IGF-1, gonadotropin and related hormones in diabetic patients. Clin Chem Lab Med, 39(7), 618-623.
3. Aliakber S, Brown PR and Bidwell DE (1993). Human erythrocyte superoxide dismutase in adults, neonates and chromosomally abnormal fetuses. Clin Biochem, 26, 109–113.
4. Aslan R, Şekeroğlu MR, Bayiroğlu F (1995). Serbest radikal türlerin membran lipid peroksidasyonuna etkileri ve hücresel antioksidan savunma. Sağ Bil Derg, 2, 137-142.
5. Bekerecioğlu M, Uğraş S, Dilek ON (1998). Serbest Radikaller. Sendrom, 10 (3), 85–94.
6. Braunwald ER, Fauci A, Kasper D, Hauser SL, Longo DL, Jameson JL (2003). Harrison’s Principles of İnternal Medicine. 12th Ed., McGraw- Hill, p: 2019-2025, New York.
7. Bunn HF (1981). Nonenzimatic glycosylation of protein, relevance of diabetes. Am J Med, 70, 325-330.
8. Carl A, Burtis R, Edward R, Ashwood MD (2003). Tietz Textbook of Clinical Chemistry. 3rd Ed., WB Saunders, p:790-796, Philadelphia.

9. Cengiz M, Cengiz S (2000). Tip II diyabetli hastalarda C vitamini uygulamasının eritrosit glutatyon ve HbA1c düzeyleri üzerine etkisi. Cerrahpaşa Tıp Derg I, 31(4), 211-215.
10. Cheeseman KH, Slater TF (1993). An introduction to free radical biochemistry. Br Med Bull, 49(3), 481-493.
11. Fairbanks VF, Klee GG (1994) Biochemical Aspects of Hematology. Second edition, WB Saunders Company, p:1974-2072, Philadephia.
12. Flohe L, Gunzler WA (1984). Assays of glutathione peroxidase, in: Methods in Enzymology, L Packer (ed), 105: p:114-115, New York.
13. Flohe L, Otting F (1984). Superoxide dismutase assays, in: Methods in Enzymology, Packer L (ed), 105: 93-104, New York.
14. Gutteridge JM (1995). Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem, 41:(12), 1819-1828.
15. Güzel S, Seven A, Civelek S, Salman S, Satman İ, Burçak G (2001). Tip I diyabetiklerin erken ve geç döneminde antioksidan statü. Cerrahpaşa Tıp Derg, 32 (4), 243-248.
16. Halifeoğlu İ, Karataş F, Çolak R, Canatan H, Telo S (2005). Tip II diyabetik hastalarda tedavi öncesi ve tedavi sonrası oksidant ve antioksidant I. Fırat Tıp Dergisi, Cilt 10, Sayı 3, 117-122.
17. Jacob RA, Burr BJ (1996). Oxidative damage and defence. Am J Clin Nut, 63, 985-990.
18. Kaneto H, Kajimoto Y, Miyagawa J, Matsuoka T, Fujitani Y, Umayahara Y, Hanafusa T, Matsuzawa Y, Yamasaki Y, Hori M (1999). Beneficial effects of antioksidant in diabetes, possible protection of pancreatic beta-cell against glucose toxicity. Diabetes, 48, 2398-2406.
19. Karabay G, Zağyapan R, Take G (2006). Streptozotosinle oluşturulan diabetin sıçan periferik sinirleri üzerine etkisinin elektron mikroskobik incelenmesi. UÜ Tıp Fak Derg, 32 (3), 77-81.
20. Kesavulu MM, Giri R, Kameswara Rao B, Apparao C (2000). Lipid peroxidation and antioxidant enzyme levels in type 2 diabetics with microvascular complications. Diabetes Metab, 26(5), 387-392.
21. Martin-Gallán P, Carrascosa A, Gussinye M, Domìnguez C (2005). Estimation of Lipoperoxidative Damage and Antioxidant Status in Diabetic Children: Relationship with Individual Antioxidant. Free Radic Res, 39(9), 933-942.
22. Matteucci E, Gıampıetro O (2000). Oxidative Stress in Families of Type 1 Diabetic Patients. Diabetes Care, 23(8), 1182-1186.
23. Mayes PA (1993). Lipids of physiologic significance, in: Harper’s Biochemistry, Lange Medical Publication, p:142–153, 588–598, London.
24. Onat T, Emerk K, Sözmen YE (2006). İnsan Biyokimyası. Palme Yayıncılık, Basım, Ankara.
25. Palanduz S, Ademoğlu E, Gökkuşu C, Tamer S (2001). Plasma antioxidants and type 2 diabetes mellitus. Res Commun Mol Pathol Pharmacol, 109(5-6), 309-318.
26. Soliman GZ (2008). Blood lipid peroxidation superoxide dismutase, malonaldehyde, glutathione levels in egyptian type 2 diabetic patients. Singapore Med J, 49(2), 129-36.
27. Stringer MD, Gorog PG, Freeman A, Kaskar VV (1989). Lipid peroxides and athero- sclerosis. BMJ, 298, 281-284.
28. Velazquez E, Winocour PH, Kesteven P, Alberti KG, Laker MF (1991). Relation of lipid peroxidase to macrovascular disease in type 2 diabetes. Diabet Med, 8, 752-758.