Yıl 2013,
Cilt: 24 Sayı: 2, 73 - 75, 01.06.2013
Öz
In this study it was aimed to determination as histochemically of some glycoconjugates in mucosa of bovine’s jejunum by Con A (Canavaliaensiformis), PNA (Arachishypogaea), UEA-1 (Ulexeuropaeus), SBA (Glycinemax) lectins. As a result of lectin histochemically methods, positive reaction was observed with all lectin in surface of villous epithelium cells. The positive reaction was obtained with the lectins with the lectins except for Con A in the goblet cells. It was detected positive reaction in villous epithelium and glands epithelial cells with Con A lectins. The reaction was observed in apical cytoplasm.
Kaynakça
- Atuma C, Strugala V, Allen A, Holm L (2002). The adherent gastrointestinal mmucus gel layer: thickness and physicalstate in vivo. Am J Physiol Gastrointest LiverPhysiol, 280, G922-G929.
- Blikslager AT, Roberts MC (1997). Mechanisms of intestinal mucosal repair. J Am Vet Med Assoc, 211, 1437-1441.
- Boonsoongnern P, Saengprapaitip K, Srisai, D, Suprasert D-A (2007). Glycoconjugates characterization in jejunal goblet cell of the chicken by means of lectin histochemistry. Kasetsart Veterinarians, 17, 2.
- Brooks SA, Carter TM (2001). N-acetylgalactosamine, N- acetylglucosamine and sialicasidexpression in primary breast cancer. Acta Histochem, 103, 37-51.
- Choi, BY, Sohn, YS, Choi C, Chae C (2003). Lectin histochemistry for glycoconjugates in the small intestines of piglets naturally infected with Isoporasuis. J Med Sci, 65, 3, 389-392, Eşrefoğlu M (2004). Genel ve Özel Histoloji. Pelikan Tıp ve Teknik Kitapçılık, İstanbul.
- Ferri D, Liquori GE, Natale L, Santarelli G, Scillitani G (2001). Mucin histochemistry of the digestive tract of the red-leggedfrog Rana aurora. Acta Histochemica, 103, 225-237.
- Filippe MI, Fenger C (1979). Histochemical characteristics of mucins in the small intestine. Acomperative study of normal mucosa, beign epithelial tumors and carcinoma. Histochem J, 11,277-287.
- Fischer J, Klein JP, Vierbuchen M, Skutta B, Uhlenbruck G, Fischer R (1984). Characterization of glycoconjugates of human gastrointesitnal mucosa by lectins. Histochem and Cytochem, 32, 7, 681-689.
- Kemper AC, Specian RD (1991). Rat small intestinal mucins a quantative analysis. Aanat Rec, 11, 277-287.
- Sancar-Baş S, Kaptan E, Sengezer-İnceli M, Sezen A, Us H (2009). Glycoconjugatehistochemistry in the fundic stomach and smallintestine of thefrog (Rana ridibunda). IUFS J Biol, 68, 2, 93-104.
- Schaumburg-Lever G, Alroy J, Ucci A, Lever WF (1984). Distribution of carbohydrate residues in normal skin. Arch Dermatol Res, 276, 216- 223.
- Scillitani G, Zizza S, Liquori GE, Ferri, D (2007). Lectin histochemistry of gastrointestinal glycoconjugates in the greater horseshoe bat, Rhinolophus ferrumequinum (Schreber, 1774). Acta Histochem, 109, 347-357.
- Stoward PJ, Spicer SS, Miller RL (1980). Histochemical Reactivity of Peanut Lectin Peroxidase Conjugate. J Histochem Cytochem, 28, 9, 979- 990.
- Strous GJ, Dekker J (1992). Mucin-type glycoproteins. Crit Rev Biochem Mol Biol, 27, 57-92.
- Tanyolaç A (1993). Özel Histoloji. Ankara Üniversitesi Veteriner Fakültesi Yayınları, Ankara.
- Vecchi T, Franchis D, Tronconni A, Agape D (1989). Evidence of altered structural and secretory glycoconjugates in the jejunal mucosa of patiens with glüten sensitive enteropathy and subtotal villous atrophy. Gut, 30, 804-810.
- Yörük M (2008): Sindirim Sistemi II: Sindirim Kanalı. Veteriner Özel Histoloji, Özer A (Ed), 161-183, Nobel Yayın Dağıtım, Ankara.
Yıl 2013,
Cilt: 24 Sayı: 2, 73 - 75, 01.06.2013
Öz
Bu çalışmada sığır jejunum mukozasındaki bazı glikokonjugatların lektin histokimyasal karakterizasyonunun Con A (Canavaliaensiformis), PNA(Arachishypogaea), UEA-I (Ulexeuropaeus) ve SBA (Glycinemax) lektinleri ile belirlenmesi amaçlandı. Lektin histokimyasal uygulamalar sonucunda villus epitel hücrelerinin yüzeyinde tüm lektinlerle pozitif reaksiyon gözlendi. Kadeh hücrelerinde Con A hariç tüm lektinlerle pozitif reaksiyon elde edildi. Villus epitel ve bez epitel hücrelerinde Con A lektini ile pozitif reaksiyon saptandı. Reaksiyon apikal sitoplazmada gözlendi.
Kaynakça
- Atuma C, Strugala V, Allen A, Holm L (2002). The adherent gastrointestinal mmucus gel layer: thickness and physicalstate in vivo. Am J Physiol Gastrointest LiverPhysiol, 280, G922-G929.
- Blikslager AT, Roberts MC (1997). Mechanisms of intestinal mucosal repair. J Am Vet Med Assoc, 211, 1437-1441.
- Boonsoongnern P, Saengprapaitip K, Srisai, D, Suprasert D-A (2007). Glycoconjugates characterization in jejunal goblet cell of the chicken by means of lectin histochemistry. Kasetsart Veterinarians, 17, 2.
- Brooks SA, Carter TM (2001). N-acetylgalactosamine, N- acetylglucosamine and sialicasidexpression in primary breast cancer. Acta Histochem, 103, 37-51.
- Choi, BY, Sohn, YS, Choi C, Chae C (2003). Lectin histochemistry for glycoconjugates in the small intestines of piglets naturally infected with Isoporasuis. J Med Sci, 65, 3, 389-392, Eşrefoğlu M (2004). Genel ve Özel Histoloji. Pelikan Tıp ve Teknik Kitapçılık, İstanbul.
- Ferri D, Liquori GE, Natale L, Santarelli G, Scillitani G (2001). Mucin histochemistry of the digestive tract of the red-leggedfrog Rana aurora. Acta Histochemica, 103, 225-237.
- Filippe MI, Fenger C (1979). Histochemical characteristics of mucins in the small intestine. Acomperative study of normal mucosa, beign epithelial tumors and carcinoma. Histochem J, 11,277-287.
- Fischer J, Klein JP, Vierbuchen M, Skutta B, Uhlenbruck G, Fischer R (1984). Characterization of glycoconjugates of human gastrointesitnal mucosa by lectins. Histochem and Cytochem, 32, 7, 681-689.
- Kemper AC, Specian RD (1991). Rat small intestinal mucins a quantative analysis. Aanat Rec, 11, 277-287.
- Sancar-Baş S, Kaptan E, Sengezer-İnceli M, Sezen A, Us H (2009). Glycoconjugatehistochemistry in the fundic stomach and smallintestine of thefrog (Rana ridibunda). IUFS J Biol, 68, 2, 93-104.
- Schaumburg-Lever G, Alroy J, Ucci A, Lever WF (1984). Distribution of carbohydrate residues in normal skin. Arch Dermatol Res, 276, 216- 223.
- Scillitani G, Zizza S, Liquori GE, Ferri, D (2007). Lectin histochemistry of gastrointestinal glycoconjugates in the greater horseshoe bat, Rhinolophus ferrumequinum (Schreber, 1774). Acta Histochem, 109, 347-357.
- Stoward PJ, Spicer SS, Miller RL (1980). Histochemical Reactivity of Peanut Lectin Peroxidase Conjugate. J Histochem Cytochem, 28, 9, 979- 990.
- Strous GJ, Dekker J (1992). Mucin-type glycoproteins. Crit Rev Biochem Mol Biol, 27, 57-92.
- Tanyolaç A (1993). Özel Histoloji. Ankara Üniversitesi Veteriner Fakültesi Yayınları, Ankara.
- Vecchi T, Franchis D, Tronconni A, Agape D (1989). Evidence of altered structural and secretory glycoconjugates in the jejunal mucosa of patiens with glüten sensitive enteropathy and subtotal villous atrophy. Gut, 30, 804-810.
- Yörük M (2008): Sindirim Sistemi II: Sindirim Kanalı. Veteriner Özel Histoloji, Özer A (Ed), 161-183, Nobel Yayın Dağıtım, Ankara.
Toplam 17 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Yazarlar | |
| Yayımlanma Tarihi | 1 Haziran 2013 |
| IZ | https://izlik.org/JA23FU29KW |
| Yayımlandığı Sayı | Yıl 2013 Cilt: 24 Sayı: 2 |