Östrüs,Anöstrüs ve Yalancı Gebelikteki Kedilerin Dişi Üreme Organlarında Plazma Hücreleri ve Alpha-Naphthyl Acetate Esterase Pozitif Lenfositlerin Dağılımı

Öz

Anahtar Kelimeler

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Östrüs,Anöstrüs ve Yalancı Gebelikteki Kedilerin Dişi Üreme Organlarında Plazma Hücreleri ve Alpha-Naphthyl Acetate Esterase Pozitif Lenfositlerin Dağılımı

Öz

Kedilerde anöstrüs, östrüs ve yalancı gebelik dönemlerinde dişi genital kanal organları ve ovaryumlarında, plasma hücresi ve alpha naphthyl acetate esterase (ANAE) positif lenfositlerin dağılımı, histokimyasal teknikler kullanılarak ışık mikroskopik olarak belirlendi. Plasma hücrelerinin incelenmesi için doku örnekleri formol-alkol solüsyonunda 48 saat tespit edilip parafinde bloklandı. Altı mikron kalınlığındaki kesitler metil green - pironin ile boyandı. ANAE için doku örnekleri formolsükroz solüsyonunda (pH 6.8) +4C ‘de 22 saat, sonra Holt solüsyonunda yine aynı şartlarda 22 saat fikse edildi. ANAE boyaması için 8 μm kalınlığındaki kriyostat kesitleri pH 6.2’de inkübe edildi. Kedilerde anöstrüs döneminde plasma hücre sayısı korpus uteride kornu uteriye göre anlamlı derecede azdı (P

Anahtar Kelimeler

• Kelimeler: Plasma hücresi, Lenfosit, Östrüs siklusu, Yalancı gebelik, Kedi

Kaynakça

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