Year 2019, Volume 6, Issue 2, Pages 165 - 173 2019-06-30

Synthesis, Characterization and Investigation of Antimicrobial Activity of Silver Nanoparticles (AgNP) from Pistachio (Pistacia vera L.) Leaf
Fıstık (Pistacia vera L.) Yaprağından Gümüş Nanopartikül (AgNP)’lerin Sentezi, Karakterizasyonu ve Antimikrobiyal Aktivitesinin İncelenmesi

Abdullah EREN [1] , Mehmet Fırat BARAN [2]

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The aim of this study is to synthesize silver nanoparticles (AgNPs) by green synthesis methods using pistachio (Pistacia vera L.) plant extract. Green synthesis is a biological method in which nanoparticles are easily obtained; as well as being environmentally friendly and inexpensive in terms of cost, and being more preferred than chemical and physical methods. AgNPs obtained by the reaction were chacarterized with UV visible spectrophotometer (UV-vis), fouriertransformed infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric and differential thermal analysis (TGA-DTA), scanning electron microscopy and energy dispersive X-ray device (SEM-EDX). Silver nanoparticles were found to have a maximum absorbance at 460.67 nm. FTIR analysis was performed to analyze the phytochemicals involved in the reduction of AgNPs. Using the Debye-Scherrer's equation, the crystal size of the synthesized nanoparticles was calculated as 16.7 nm. AgNPs spherical appearance were detected by transmission electron microscopy. The antimicrobial effect of synthesized AgNPs were tested on gram-positive and gram-negative bacteria, and by the minimum inhibitory concentration (MIC) method on fungi (yeast). As a result, it was determined that the synthesized silver nanoparticles had antimicrobial effect.



Bu çalışmanın amacı, fıstık (Pistacia vera L.) bitki ekstraktı kullanılarak, yeşil sentez yöntemi ile gümüş nanopartikül (AgNP)’lerini sentezlemektir. Yeşil sentez; çevre dostu ve maliyet açısından ucuz olması, kimyasal ve fiziksel yöntemlerden daha çok tercih edilen nanopartiküllerin kolay bir şekilde elde edilmesi ile bilinen biyolojik bir yöntemdir. Reaksiyon sonucunda elde edilen AgNP’ler, UV görünür spektrofotometre (UV-vis), fourier-dönüştürülmüş kızılötesi spektroskopisi (FTIR), X-Işını kırınımı (XRD), termogravimetrik ve diferansiyel termal analizi (TGA-DTA), taramalı elektron mikroskobu ve enerji yayılımlı X-ışını cihazı (SEM-EDX) kullanılarak karakterize edilmiştir. Gümüş nanopartiküllerin 460.67 nm’de maksimum absorbansa sahip olduğu görülmüştür. AgNP’lerin indirgenmesinde rol olan fitokimyasalları analiz etmek için FTIR analizi yapılmıştır. Debye-Scherrer’s denkleminden yararlanarak sentezlenen nanoparçacıkların kristal boyutunun 16.7 nm olduğu hesaplanmıştır. AgNP’lerin küresel görünümde olduğu transmisyon elektron mikroskobu ile tespit edilmiştir. Sentezlenen AgNP’lerin antimikrobiyal etkisi gram pozitif ve gram negatif bakterileri ve fungus (maya) üzerinde minimum inhibitör konsantrasyonu (MIC) metodu ile test edilmiştir. Sonuç olarak, sentezlenen gümüş nanopartiküllerin antimikrobiyal etki gösterdiği belirlenmiştir.

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Primary Language tr
Subjects Science
Published Date Haziran 2019
Journal Section Research Article
Authors

Orcid: 0000-0003-1187-7978
Author: Abdullah EREN (Primary Author)
Institution: Artuklu Üniversitesi, Kızıltepe Meslek Yüksekokulu, Organik Tarım Bölümü, Mardin
Country: Turkey


Orcid: 0000-0001-8133-6670
Author: Mehmet Fırat BARAN
Institution: Artuklu Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Hizmetler Bölümü, Mardin
Country: Turkey


Dates

Publication Date: June 30, 2019

Bibtex @research article { tutad493006, journal = {Türkiye Tarımsal Araştırmalar Dergisi}, issn = {2148-2306}, eissn = {2528-858X}, address = {Siirt University}, year = {2019}, volume = {6}, pages = {165 - 173}, doi = {10.19159/tutad.493006}, title = {Fıstık (Pistacia vera L.) Yaprağından Gümüş Nanopartikül (AgNP)’lerin Sentezi, Karakterizasyonu ve Antimikrobiyal Aktivitesinin İncelenmesi}, key = {cite}, author = {EREN, Abdullah and BARAN, Mehmet Fırat} }
APA EREN, A , BARAN, M . (2019). Fıstık (Pistacia vera L.) Yaprağından Gümüş Nanopartikül (AgNP)’lerin Sentezi, Karakterizasyonu ve Antimikrobiyal Aktivitesinin İncelenmesi. Türkiye Tarımsal Araştırmalar Dergisi, 6 (2), 165-173. DOI: 10.19159/tutad.493006
MLA EREN, A , BARAN, M . "Fıstık (Pistacia vera L.) Yaprağından Gümüş Nanopartikül (AgNP)’lerin Sentezi, Karakterizasyonu ve Antimikrobiyal Aktivitesinin İncelenmesi". Türkiye Tarımsal Araştırmalar Dergisi 6 (2019): 165-173 <http://dergipark.org.tr/tutad/issue/44154/493006>
Chicago EREN, A , BARAN, M . "Fıstık (Pistacia vera L.) Yaprağından Gümüş Nanopartikül (AgNP)’lerin Sentezi, Karakterizasyonu ve Antimikrobiyal Aktivitesinin İncelenmesi". Türkiye Tarımsal Araştırmalar Dergisi 6 (2019): 165-173
RIS TY - JOUR T1 - Fıstık (Pistacia vera L.) Yaprağından Gümüş Nanopartikül (AgNP)’lerin Sentezi, Karakterizasyonu ve Antimikrobiyal Aktivitesinin İncelenmesi AU - Abdullah EREN , Mehmet Fırat BARAN Y1 - 2019 PY - 2019 N1 - doi: 10.19159/tutad.493006 DO - 10.19159/tutad.493006 T2 - Türkiye Tarımsal Araştırmalar Dergisi JF - Journal JO - JOR SP - 165 EP - 173 VL - 6 IS - 2 SN - 2148-2306-2528-858X M3 - doi: 10.19159/tutad.493006 UR - https://doi.org/10.19159/tutad.493006 Y2 - 2019 ER -
EndNote %0 Turkish Journal of Agricultural Research Fıstık (Pistacia vera L.) Yaprağından Gümüş Nanopartikül (AgNP)’lerin Sentezi, Karakterizasyonu ve Antimikrobiyal Aktivitesinin İncelenmesi %A Abdullah EREN , Mehmet Fırat BARAN %T Fıstık (Pistacia vera L.) Yaprağından Gümüş Nanopartikül (AgNP)’lerin Sentezi, Karakterizasyonu ve Antimikrobiyal Aktivitesinin İncelenmesi %D 2019 %J Türkiye Tarımsal Araştırmalar Dergisi %P 2148-2306-2528-858X %V 6 %N 2 %R doi: 10.19159/tutad.493006 %U 10.19159/tutad.493006
ISNAD EREN, Abdullah , BARAN, Mehmet Fırat . "Fıstık (Pistacia vera L.) Yaprağından Gümüş Nanopartikül (AgNP)’lerin Sentezi, Karakterizasyonu ve Antimikrobiyal Aktivitesinin İncelenmesi". Türkiye Tarımsal Araştırmalar Dergisi 6 / 2 (June 2019): 165-173. https://doi.org/10.19159/tutad.493006
AMA EREN A , BARAN M . Fıstık (Pistacia vera L.) Yaprağından Gümüş Nanopartikül (AgNP)’lerin Sentezi, Karakterizasyonu ve Antimikrobiyal Aktivitesinin İncelenmesi. TÜTAD. 2019; 6(2): 165-173.
Vancouver EREN A , BARAN M . Fıstık (Pistacia vera L.) Yaprağından Gümüş Nanopartikül (AgNP)’lerin Sentezi, Karakterizasyonu ve Antimikrobiyal Aktivitesinin İncelenmesi. Türkiye Tarımsal Araştırmalar Dergisi. 2019; 6(2): 173-165.