The Use of DNA Barcoding in Entomology
Yıl 2018,
Cilt: 28 Sayı: 1, 126 - 134, 30.03.2018
Gökhan Yatkın
,
Nurper Güz
Öz
DNA barcoding is a
taxonomic method that uses a short and standard genomic DNA fragment to
discriminate species. An approximately 600-700 base pair (bp) region of the
mitochondrial cytochrome c oxidase
subunit I (COI) gene referred as
‘DNA barcode’ is used in insect barcoding studies. This short standart DNA
sequence is not only a tool for simplifying species identification but also is
used for discovery of new species. Furthermore DNA barcoding enables the
detection of cryptic species via delimiting species boundaries. Here we
reviewed the historical and conceptual perspective of DNA barcoding term, the
gene regions used as DNA barcodes and COI
gene which is ideal species-identification
marker in insects. Furthermore we
discussed the advantages and limitations of DNA barcoding and presented DNA
barcoding studies in entomology.
Kaynakça
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Entomolojide DNA Barkodlama Tekniğinin Kullanımı
Yıl 2018,
Cilt: 28 Sayı: 1, 126 - 134, 30.03.2018
Gökhan Yatkın
,
Nurper Güz
Öz
DNA barkodlama, herhangi
bir organizmaya ait DNA’nın PCR teknikleri ile çoğaltılacak büyüklükteki kısa
ve standart bir fragmanının dizilenmesi ile tanılanmasını sağlayan taksonomik
bir yöntem olarak ifade edilebilir. Bu amaçla böceklerle yapılan çalışmalarda
‘DNA barkodu’ olarak bilinen mitokondriyal sitokrom
c oksidaz alt ünite I (COI)
geninin yaklaşık 600-700 baz çiftine (bç) ait kısmı kullanılmaktadır. Bu kısa
standart DNA sekansı türlerin tanılanmasını kolaylaştırmasının yanı sıra yeni
türlerin keşfi için de oldukça iyi bir araçtır. Ayrıca DNA barkodlama türlerin
sınırlarını belirleyerek kriptik türlerin ayrımına katkı sağlar. Bu derlemede
DNA barkodlama teriminin tarihsel ve kavramsal olarak ortaya çıkışı, DNA barkodu
olarak kullanılan gen bölgeleri ve böcekler için ideal tür tanımlama markörü
olan COI gen bölgesi hakkında bilgi
verilmiştir. Son olarak DNA barkodlamanın avantajları ve kısıtlamaları
irdelenerek entomolojide DNA barkodlama çalışmalarından örnekler sunulmuştur.
Kaynakça
- 1. Ahrens D, Monaghan MT, Vogler AP (2007). DNA-based taxonomy for associating adults and larvae in multi-species assemblages of chafers (Coleoptera: Scarabaeidae). Molecular Phylogenetics and Evolution. 44: 436-449.
- 2. Alberts B, Johnson A, Lewis J, Raff R, Roberts K, Walter P (2002). The genetic systems of mitochondria and plastids. Molecular Biology of the Cell. 4th Edn. New York: Garland Science.
- 3. Amaral AR, Sequeira M, Coelho MM (2007). A first approach to the usefulness of cytochrome c oxidase I barcodes in the identification of closely related delphinid cetacean species. Mar. Freshwater Res. 58: 505-510.
- 4. Armstrong K, Ball S (2005). DNA barcodes for biosecurity: Invasive species identification. Philosophical Transactions of the Royal Society of London B: Biological Sciences 360: 1813-1823.
- 5. Asahida T, Yamashita Y, Kabayashi T (1997). Identification of consumed stone flounder Kareius bicoloratus (Basilewsky), from the stomach contents of sand shrimp, Crangon affinis (De Hann) using mitochondrial DNA analysis. Journal of Experimental Marine Biology and Ecology. 217, 153-163.
- 6. Ball SL, Armstrong KF (2006). DNA barcodes for insect pest identification: a test case with tussock moths (Lepidoptera: Lymantriidae). Canadian Journal of Forest Research. 36: 337-350.
- 7. Beutel RG, Pohl H (2006). Endopterygote systematics– where do we stand and what is the goal (Hexapoda, Arthropoda)? Systematic Entomology. 31: 202-219.
- 8. Blaxter M (2003). Counting angels with DNA. Nature. 421: 22-124.
- 9. Blaxter ML (2004). The promise of a DNA taxonomy. Philos Trans R Soc Lond B Biol Sci. 359: 669-679.
- 10. Busse HJ, Denner EBM, Lubitz W (1996). Classification and identification of bacteria: current approaches to an old problem. Overview of methods used in bacterial systematics. Journal of Biotechnology. 47: 3-38.
- 11. Cywinska A, Hunter FF, Hebert P.D.N (2006). Identifying Canadian mosquito species through DNA barcodes. Medical and Veterinary Entomology. 20: 413-424.
- 12. Çakmak I, Janssen A, Sabelis MW (2006). Intraguild interactions between the predatory mites Neoseiulus californicus and Phytoseiulus persimilis. Exp. and Appl. Acarol. 38: 33-46.
- 13. Dasmahapatra KK, Mallet J (2006). Taxonomy: DNA barcodes: recent successes and future prospects. Heredity (Edinb). 97: 254-255.
- 14. Duman M, Guz N, Sertkaya E (2015). DNA barcoding of sunn pest adult parasitoids using cytochrome c oxidase subunit I (COI). Biochem. Syst. Ecol. 59 70-77.
- 15. Dunshea G (2009). DNA-based diet analysis for any predator. PLoS One 4:e5252.
- 16. Farrell LE, Roman J, Sunquist ME (2000). Dietary separation of sympatric carnivores identified by molecular analysis of scats. Mol Ecol. 9: 1583-90.
- 17. Franck P, Maalouly-Matar M, Olivares J (2017). Molecular Tools for the Detection and the Identification of Hymenoptera Parasitoids in Tortricid Fruit Pests. Int. J. Mol. Sci. 18, 2031.
- 18. Foley DH, Wilkerson RC, Cooper RD, Volovsek ME, Bryan JH (2007). A molecular phylogeny of Anopheles annulipes (Diptera: Culicidae) sensu lato: the most species-rich anopheline complex. Molecular Phylogenetics and Evolution. 43: 283-297.
- 19. Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol. Mar. Biol. Biotech. 3, 294-299.
- 20. Fournier V, Hagler J, Daane K, de Leon J, Groves R (2008). Identifying the predator complex of Homalodisca vitripennis (Hemiptera: Cicadellidae): A comparative study of the efficacy of an ELISA and PCR gut content assay. Oecologia 157:629-40.
- 21. Funk DJ, Omland KE (2003). Species-level paraphyly and polyphyly: Frequency, causes, consequences, with insights from animal mitochondrial DNA. Annu Rev Ecol Syst. 34: 397-423.
- 22. Gariepy TD, Kuhlmann U, Gillott C, Erlandson M (2007). Parasitoids, predators and PCR: the use of diagnostic molecular markers in biological control of arthropods. J. Appl. Entomol. 131: 225-240.
- 23. Gariepy TD, Haye T, Zhang J (2014). A molecular diagnostic tool for the preliminary assessment of host–parasitoid associations in biological control programmes for a new invasive pest. Mol Ecol. 23: 3912-3924.
- 24. Gattolliat JL, Monaghan MT (2010). 'DNA‐based association of adults and larvae in Baetidae (Ephemeroptera) with the description of a new genus Adnoptilum in Madagascar'. Journal of the North American Benthological Society. 29 1042-1057.
- 25. Godfray HCJ (1994). Parasitoids: Behavioral and Evolutionary Ecology. Princeton University Press, Princeton, New Jersey.
- 26. Gomez A, Wright PJ, Lunt DH, Cancino JM, Carvalho GR, Hughes RN (2007). Mating trials validate the use of DNA barcoding to reveal cryptic speciation of a marine bryozoan taxon. Proc R Soc Biol Sci Ser B. 274: 199-207.
- 27. Grimaldi DA, Engel MS (2005). Evolution of the Insects. Cambridge University Press, Cambridge.
- 28. Guz N, Kocak E, Kilincer N (2013). Molecular phylogeny of Trissolcus species (Hymenoptera: Scelionidae). Biochemical Systematics and Ecology 48, 85-91.
- 29. Hajibabaei M, Janzen DH, Burns JM, Hallwachs W, Hebert PDN (2006). DNA barcodes distinguish species of tropical Lepidoptera. Proceedings of the National Academy of Sciences of the United States of America. 103: 968-971.
- 30. Hayashi M, Sota T (2010) Identification of elmid larvae (Coleoptera: Elmidae) from Sanin District of Honshu, Japan, based on mitochondrial DNA sequences. Entomological Science. 13 417-424.
- 31. Hebert PDN, Cywinska A, Ball SL, deWaard JR (2003a). Biological identifications through DNA barcodes. Proceedings of the Royal Society. B. Biological Sciences. 270: 313-321.
- 32. Hebert PDN, Ratnasingham S, deWaard JR (2003b). Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proc R Soc B, 270: 96-99.
- 33. Hebert PDN, Penton EH, Burns JM, Janzen DH, Hallwachs W (2004). Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proc Natl Acad Sci USA. 101: 14812-14817.
- 34. Hogg ID, Hebert PDN (2004). Biological identification of springtails (Hexapoda: Collembola) from the Canadian Arctic, using mitochondrial DNA barcodes. Canadian Journal of Zoology. 82: 749-754.
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