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Evaluation of the Erythrocyte Fragility, Haematological Parameters and Antioxidant Properties of Platanus orientalis Leaf Infusion Against Ethanol Toxicity in Rats

Year 2020, Volume: 35 Issue: 1, 22 - 28, 30.06.2020
https://doi.org/10.28955/alinterizbd.740369

Abstract

The aim of this study was to evaluate the protective effects of the leaf infusion of chinar (Platanus orientalis L.) on erythrocyte fragility, haematological parameters and antioxidant status against ethanol-induced oxidative stress in rats. Thirty male rats were divided into five groups: Control, Ethanol, Ethanol+Silymarin (10 mg kg-1), Ethanol+PO-20 mg mL-1 infusion, and Ethanol+PO-60 mg mL-1 infusion. According to the results, in the Ethanol group, erythrocyte counts, red cells distribution, plateletcrit, platelet and lymphocyte levels significantly decreased compared to the Control group, while PO-60 dose-fed group showed a significant increase in haematocrit and haemoglobin values compared to the Ethanol group. There were significant changes in erythrocyte fragility of Ethanol and Ethanol-treatment groups at different NaCl concentrations of 0.3 and 0.6 according to Control group. It was observed that PO Leaf infusion reduced the hemolysis caused by ethanol at a concentration of 0.3% NaCl, thus reducing the values to the control values. In addition, PO leaf infusion caused a significant increase in total antioxidant status against ethanol toxicity and a significant decrease in total oxidative status and oxidative stress index. It was concluded that PO leaf infusion may have antihematotoxic effect, reducing erythrocyte fragility and increase antioxidant capacity against ethanol toxicity.

Supporting Institution

Van Yuzuncu Yil University Scientific Research Projects Department

Project Number

TAP-2019-8183 and TYL-2018-6774

Thanks

This study was financially supported by Van Yuzuncu Yil University Scientific Research Projects Department. Project Numbers (TAP-2019-8183 and TYL-2018-6774).

References

  • Alimi, H., Hfaeidh, N., Bouoni, Z., Sakly, M., and Rhouma, K.B., 2012. Protective effect of Opuntia ficus indica f. inermis prickly pear juice upon ethanol-induced damages in rat erythrocytes. Alcohol 46(3): 235-243.
  • Arihan, O., Oto, G., Bayram, I., and Aras, I. 2016. Effect of safran, safranal and crocin which are active ingredients of Saffron (Crocus) on erythrocyte fragility and hematological parameters in carbon tetrachloride intoxicated rats. Eastern Journal of Medicine 21(4): 173.
  • Bati, B., Celik, I., and Dogan, A., 2015. Determination of hepatoprotective and antioxidant role of walnuts against ethanol-induced oxidative stress in rats. Cell biochemistry and biophysics 71(2): 1191-1198.
  • Chatzigeorgiou, S., Thai, Q. D., Tchoumtchoua, J., Tallas, K., Tsakiri, E.N., Papassideri, I., and Trougakos, I. P., 2017. Isolation of natural products with anti-ageing activity from the fruits of Platanus orientalis. Phytomedicine 33: 53-61.
  • Chiu, C. H., Wang, Y. C., Yeh, K. M., Lin, J. C., Siu, L. K., and Chang, F. Y., 2018. Influence of ethanol concentration in the phagocytic function of neutrophils against Klebsiella pneumoniae isolates in an experimental model. Journal of microbiology, immunology and infection 51(1): 64-69.
  • Chopra, R. N., Nayar, S. L., and Chopra, I. C., 1956. Glossary of Indian medicinal Plants (Council of Industrial and Scientific Research). New Delhi 1: 133.
  • da Cunha, F. A. B., Waczuk, E. P., Duarte, A. E., Barros, L. M., Elekofehinti, O. O., Matias, E. F. F., and Souza, D. O., 2016. Cytotoxic and antioxidative potentials of ethanolic extract of Eugenia uniflora L. (Myrtaceae) leaves on human blood cells. Biomedicine Pharmacotherapy 84: 614-621.
  • Dogan, A., 2018. Lyophilized Extract of the Horse Mushroom, Agaricus arvensis (Agaricomycetes) Delayes Erythrocyte Hemolysis and Stabilizes Some Blood Parameters in Carbontetrachloride-Intoxicated Rats. International journal of medicinal mushrooms 20: 909-918.
  • Dogan, A., and Anuk, O. O., 2019. Investigation of the phytochemical composition and antioxidant properties of chinar (Platanus orientalis L.) leaf infusion against ethanol-induced oxidative stress in rats. Molecular biology reports 46(3): 3049-3061.
  • Dogan, A., and Celik, I., 2012. Hepatoprotective and antioxidant activities of grapeseeds against ethanol-induced oxidative stress in rats. British Journal of Nutrition 107(1): 45-51.
  • Erel, O., 2004. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical biochemistry 37(4): 277–285.
  • Erel, O., 2005. A new automated colorimetric method for measuring total oxidant status. Clinical biochemistry 38(12): 1103–1111.
  • Erkec, O. E., Arihan, O., Colcimen, N., Kara, M., Karatas, E., Demir, H., and Ragbetli, M. C., 2018. Effects of Cichorium intybus on serum oxidative stress, liver and kidney volume, and cyclin B1 and Bcl-2 levels in the brains of rats with ethanol induced damage. Cell Mol Biol (Noisy le Grand) 64(7): 30-35.
  • Gao, X., Yin, J., Wang, X., Petersen, F., and Yu, X., 2019. A comprehensive comparison of hematological parameters among 39 common diseases. Scandinavian journal of clinical and laboratory investigation 79(4): 251-259.
  • Haider, S., Nazreen, S., Alam, M. M., Hamid, H., and Alam, M. S., 2012. Anti-inflammatory and anti-nociceptive activities of Platanus orientalis Linn. and its ulcerogenic risk evaluation. Journal of ethnopharmacology 143(1): 236-240.
  • Khan, A. S., 2017. Woody plants with possible anti-HIV activity. In: Medicinally Important Trees Springer, Cham, pp. 109-131.
  • King, M. J., Garçon, L., Hoyer, J. D., Iolascon, A., Picard, V., Stewart, G., and International Council for Standardization in Haematology, 2015. ICSH guidelines for the laboratory diagnosis of nonimmune hereditary red cell membrane disorders. International journal of laboratory hematology 37(3): 304-325.
  • Lu, Y., and Cederbaum, A. I., 2008. CYP2E1 and oxidative liver injury by alcohol. Free Radical Biology and Medicine 44(5): 723-738.
  • Mitrokotsa, D., Mitaku, S., Demetzos, C., Harvala, C., Mentis, A., Perez, S., and Kokkinopoulos, D., 1993. Bioactive compounds from the buds of Platanus orientalis and isolation of a new kaempferol glycoside. Planta Medica 59(6): 517-520.
  • Olchowik, E., Lotkowski, K., Mavlyanov, S., Abdullajanova, N., Ionov, M., Bryszewska, M., and Zamaraeva, M., 2012. Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.). Cellular Molecular Biology Letters 17(3): 333.
  • Ozkol, H., Bulut, G., Balahoroglu, R., Tuluce, Y., and Ozkol, H. U., 2017. Protective effects of selenium, N-acetylcysteine and vitamin E against acute ethanol intoxication in rats. Biological trace element research 175(1): 177-185.
  • Padmini, E., and Sundari, B. T., 2008. Erythrocyte glutathione depletion impairs resistance to haemolysis in women consuming alcohol. Journal of clinical biochemistry and nutrition 42 (1): 14-20.
  • Pari, L., and Suresh, A., 2008. Effect of grape (Vitis vinifera L.) leaf extract on alcohol induced oxidative stress in rats. Food and chemical toxicology 46(5): 1627-1634.
  • Shaker, E., Mahmoud, H., and Mnaa, S., 2010. Silymarin, the antioxidant component and Silybum marianum extracts prevent liver damage. Food and Chemical Toxicology 48(3): 803-806.
  • Sinha, N., and Dabla, K. P., 2015. Oxidative stress and antioxidants in hypertension–a current review. Current hypertension reviews 11(2): 132-142.
  • Tamura, R., Ohta, H., Satoh, Y., Nonoyama, S., Nishida, Y., and Nibuya, M., 2016. Neuroprotective effects of adenosine deaminase in the striatum. Journal of Cerebral Blood Flow Metabolism 36(4): 709-720.
  • Tülüce, Y., Lak, P. T. A., Koyuncu, İ., Kılıç, A., Durgun, M., and Özkol, H., 2017. The apoptotic, cytotoxic and genotoxic effect of novel binuclear boron-fluoride complex on endometrial cancer. BioMetals 30(6): 933-944.
  • Tyulina, O. V., Prokopieva, V. D., Boldyrev, A. A., and Johnson, P., 2006. Erythrocyte and plasma protein modification in alcoholism: a possible role of acetaldehyde. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease 1762(5): 558-563.
  • Varga, Z. V., Matyas, C., Paloczi, J., and Pacher, P., 2017. Alcohol misuse and kidney injury: epidemiological evidence and potential mechanisms. Alcohol research: current reviews 38(2): 283.
  • Wrońska‐Noferδ, T., Rosin, J., and Bartosz, G., 1991. Interaction of ethanol and xylene in their effects on erythrocytes and other haematological parameters in the rat. Journal of Applied Toxicology 11(4): 289-292.
  • Zakhari, S., 2006. Overview: how is alcohol metabolized by the body. Alcohol Research 29(4): 245.
Year 2020, Volume: 35 Issue: 1, 22 - 28, 30.06.2020
https://doi.org/10.28955/alinterizbd.740369

Abstract

Project Number

TAP-2019-8183 and TYL-2018-6774

References

  • Alimi, H., Hfaeidh, N., Bouoni, Z., Sakly, M., and Rhouma, K.B., 2012. Protective effect of Opuntia ficus indica f. inermis prickly pear juice upon ethanol-induced damages in rat erythrocytes. Alcohol 46(3): 235-243.
  • Arihan, O., Oto, G., Bayram, I., and Aras, I. 2016. Effect of safran, safranal and crocin which are active ingredients of Saffron (Crocus) on erythrocyte fragility and hematological parameters in carbon tetrachloride intoxicated rats. Eastern Journal of Medicine 21(4): 173.
  • Bati, B., Celik, I., and Dogan, A., 2015. Determination of hepatoprotective and antioxidant role of walnuts against ethanol-induced oxidative stress in rats. Cell biochemistry and biophysics 71(2): 1191-1198.
  • Chatzigeorgiou, S., Thai, Q. D., Tchoumtchoua, J., Tallas, K., Tsakiri, E.N., Papassideri, I., and Trougakos, I. P., 2017. Isolation of natural products with anti-ageing activity from the fruits of Platanus orientalis. Phytomedicine 33: 53-61.
  • Chiu, C. H., Wang, Y. C., Yeh, K. M., Lin, J. C., Siu, L. K., and Chang, F. Y., 2018. Influence of ethanol concentration in the phagocytic function of neutrophils against Klebsiella pneumoniae isolates in an experimental model. Journal of microbiology, immunology and infection 51(1): 64-69.
  • Chopra, R. N., Nayar, S. L., and Chopra, I. C., 1956. Glossary of Indian medicinal Plants (Council of Industrial and Scientific Research). New Delhi 1: 133.
  • da Cunha, F. A. B., Waczuk, E. P., Duarte, A. E., Barros, L. M., Elekofehinti, O. O., Matias, E. F. F., and Souza, D. O., 2016. Cytotoxic and antioxidative potentials of ethanolic extract of Eugenia uniflora L. (Myrtaceae) leaves on human blood cells. Biomedicine Pharmacotherapy 84: 614-621.
  • Dogan, A., 2018. Lyophilized Extract of the Horse Mushroom, Agaricus arvensis (Agaricomycetes) Delayes Erythrocyte Hemolysis and Stabilizes Some Blood Parameters in Carbontetrachloride-Intoxicated Rats. International journal of medicinal mushrooms 20: 909-918.
  • Dogan, A., and Anuk, O. O., 2019. Investigation of the phytochemical composition and antioxidant properties of chinar (Platanus orientalis L.) leaf infusion against ethanol-induced oxidative stress in rats. Molecular biology reports 46(3): 3049-3061.
  • Dogan, A., and Celik, I., 2012. Hepatoprotective and antioxidant activities of grapeseeds against ethanol-induced oxidative stress in rats. British Journal of Nutrition 107(1): 45-51.
  • Erel, O., 2004. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical biochemistry 37(4): 277–285.
  • Erel, O., 2005. A new automated colorimetric method for measuring total oxidant status. Clinical biochemistry 38(12): 1103–1111.
  • Erkec, O. E., Arihan, O., Colcimen, N., Kara, M., Karatas, E., Demir, H., and Ragbetli, M. C., 2018. Effects of Cichorium intybus on serum oxidative stress, liver and kidney volume, and cyclin B1 and Bcl-2 levels in the brains of rats with ethanol induced damage. Cell Mol Biol (Noisy le Grand) 64(7): 30-35.
  • Gao, X., Yin, J., Wang, X., Petersen, F., and Yu, X., 2019. A comprehensive comparison of hematological parameters among 39 common diseases. Scandinavian journal of clinical and laboratory investigation 79(4): 251-259.
  • Haider, S., Nazreen, S., Alam, M. M., Hamid, H., and Alam, M. S., 2012. Anti-inflammatory and anti-nociceptive activities of Platanus orientalis Linn. and its ulcerogenic risk evaluation. Journal of ethnopharmacology 143(1): 236-240.
  • Khan, A. S., 2017. Woody plants with possible anti-HIV activity. In: Medicinally Important Trees Springer, Cham, pp. 109-131.
  • King, M. J., Garçon, L., Hoyer, J. D., Iolascon, A., Picard, V., Stewart, G., and International Council for Standardization in Haematology, 2015. ICSH guidelines for the laboratory diagnosis of nonimmune hereditary red cell membrane disorders. International journal of laboratory hematology 37(3): 304-325.
  • Lu, Y., and Cederbaum, A. I., 2008. CYP2E1 and oxidative liver injury by alcohol. Free Radical Biology and Medicine 44(5): 723-738.
  • Mitrokotsa, D., Mitaku, S., Demetzos, C., Harvala, C., Mentis, A., Perez, S., and Kokkinopoulos, D., 1993. Bioactive compounds from the buds of Platanus orientalis and isolation of a new kaempferol glycoside. Planta Medica 59(6): 517-520.
  • Olchowik, E., Lotkowski, K., Mavlyanov, S., Abdullajanova, N., Ionov, M., Bryszewska, M., and Zamaraeva, M., 2012. Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves (Rhus typhina L.) and grape seeds (Vitis vinifera L.). Cellular Molecular Biology Letters 17(3): 333.
  • Ozkol, H., Bulut, G., Balahoroglu, R., Tuluce, Y., and Ozkol, H. U., 2017. Protective effects of selenium, N-acetylcysteine and vitamin E against acute ethanol intoxication in rats. Biological trace element research 175(1): 177-185.
  • Padmini, E., and Sundari, B. T., 2008. Erythrocyte glutathione depletion impairs resistance to haemolysis in women consuming alcohol. Journal of clinical biochemistry and nutrition 42 (1): 14-20.
  • Pari, L., and Suresh, A., 2008. Effect of grape (Vitis vinifera L.) leaf extract on alcohol induced oxidative stress in rats. Food and chemical toxicology 46(5): 1627-1634.
  • Shaker, E., Mahmoud, H., and Mnaa, S., 2010. Silymarin, the antioxidant component and Silybum marianum extracts prevent liver damage. Food and Chemical Toxicology 48(3): 803-806.
  • Sinha, N., and Dabla, K. P., 2015. Oxidative stress and antioxidants in hypertension–a current review. Current hypertension reviews 11(2): 132-142.
  • Tamura, R., Ohta, H., Satoh, Y., Nonoyama, S., Nishida, Y., and Nibuya, M., 2016. Neuroprotective effects of adenosine deaminase in the striatum. Journal of Cerebral Blood Flow Metabolism 36(4): 709-720.
  • Tülüce, Y., Lak, P. T. A., Koyuncu, İ., Kılıç, A., Durgun, M., and Özkol, H., 2017. The apoptotic, cytotoxic and genotoxic effect of novel binuclear boron-fluoride complex on endometrial cancer. BioMetals 30(6): 933-944.
  • Tyulina, O. V., Prokopieva, V. D., Boldyrev, A. A., and Johnson, P., 2006. Erythrocyte and plasma protein modification in alcoholism: a possible role of acetaldehyde. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease 1762(5): 558-563.
  • Varga, Z. V., Matyas, C., Paloczi, J., and Pacher, P., 2017. Alcohol misuse and kidney injury: epidemiological evidence and potential mechanisms. Alcohol research: current reviews 38(2): 283.
  • Wrońska‐Noferδ, T., Rosin, J., and Bartosz, G., 1991. Interaction of ethanol and xylene in their effects on erythrocytes and other haematological parameters in the rat. Journal of Applied Toxicology 11(4): 289-292.
  • Zakhari, S., 2006. Overview: how is alcohol metabolized by the body. Alcohol Research 29(4): 245.
There are 31 citations in total.

Details

Primary Language English
Subjects Agricultural, Veterinary and Food Sciences
Journal Section Research Articles
Authors

Abdulahad Doğan This is me 0000-0002-5438-8560

Fatih Dönmez This is me 0000-0003-3958-1028

Özgür Ozan Anuk This is me 0000-0002-9028-7118

Project Number TAP-2019-8183 and TYL-2018-6774
Publication Date June 30, 2020
Acceptance Date March 1, 2020
Published in Issue Year 2020 Volume: 35 Issue: 1

Cite

APA Doğan, A., Dönmez, F., & Anuk, Ö. O. (2020). Evaluation of the Erythrocyte Fragility, Haematological Parameters and Antioxidant Properties of Platanus orientalis Leaf Infusion Against Ethanol Toxicity in Rats. Alinteri Journal of Agriculture Science, 35(1), 22-28. https://doi.org/10.28955/alinterizbd.740369
AMA Doğan A, Dönmez F, Anuk ÖO. Evaluation of the Erythrocyte Fragility, Haematological Parameters and Antioxidant Properties of Platanus orientalis Leaf Infusion Against Ethanol Toxicity in Rats. Alinteri Journal of Agriculture Science. June 2020;35(1):22-28. doi:10.28955/alinterizbd.740369
Chicago Doğan, Abdulahad, Fatih Dönmez, and Özgür Ozan Anuk. “Evaluation of the Erythrocyte Fragility, Haematological Parameters and Antioxidant Properties of Platanus Orientalis Leaf Infusion Against Ethanol Toxicity in Rats”. Alinteri Journal of Agriculture Science 35, no. 1 (June 2020): 22-28. https://doi.org/10.28955/alinterizbd.740369.
EndNote Doğan A, Dönmez F, Anuk ÖO (June 1, 2020) Evaluation of the Erythrocyte Fragility, Haematological Parameters and Antioxidant Properties of Platanus orientalis Leaf Infusion Against Ethanol Toxicity in Rats. Alinteri Journal of Agriculture Science 35 1 22–28.
IEEE A. Doğan, F. Dönmez, and Ö. O. Anuk, “Evaluation of the Erythrocyte Fragility, Haematological Parameters and Antioxidant Properties of Platanus orientalis Leaf Infusion Against Ethanol Toxicity in Rats”, Alinteri Journal of Agriculture Science, vol. 35, no. 1, pp. 22–28, 2020, doi: 10.28955/alinterizbd.740369.
ISNAD Doğan, Abdulahad et al. “Evaluation of the Erythrocyte Fragility, Haematological Parameters and Antioxidant Properties of Platanus Orientalis Leaf Infusion Against Ethanol Toxicity in Rats”. Alinteri Journal of Agriculture Science 35/1 (June 2020), 22-28. https://doi.org/10.28955/alinterizbd.740369.
JAMA Doğan A, Dönmez F, Anuk ÖO. Evaluation of the Erythrocyte Fragility, Haematological Parameters and Antioxidant Properties of Platanus orientalis Leaf Infusion Against Ethanol Toxicity in Rats. Alinteri Journal of Agriculture Science. 2020;35:22–28.
MLA Doğan, Abdulahad et al. “Evaluation of the Erythrocyte Fragility, Haematological Parameters and Antioxidant Properties of Platanus Orientalis Leaf Infusion Against Ethanol Toxicity in Rats”. Alinteri Journal of Agriculture Science, vol. 35, no. 1, 2020, pp. 22-28, doi:10.28955/alinterizbd.740369.
Vancouver Doğan A, Dönmez F, Anuk ÖO. Evaluation of the Erythrocyte Fragility, Haematological Parameters and Antioxidant Properties of Platanus orientalis Leaf Infusion Against Ethanol Toxicity in Rats. Alinteri Journal of Agriculture Science. 2020;35(1):22-8.