Molecular Phylogeny and Historical Biogeography of Petroleuciscus (Teleostei: Leuciscidae) Species in Turkey
Yıl 2022,
Cilt: 7 Sayı: 1, 88 - 95, 31.03.2022
Gökhan Kalaycı
Öz
In this study, phylogenetic relationships of Petroleuciscus species in Turkey were investigated by using mitochondrial cytochrome b (cyt b) (1140 bp). For this purpose, 84 samples from 13 populations for the cyt b gene were sequenced. The result of of genetic analysis, twenty-one haplotypes were identified and overall haplotype diversity was calculated as Hd: 0.921±0.011. The result of phylogenetic analysis of Petroleuciscus species shows the presence of two clades and four haplogroups. First clade includes Petroleuciscus smyrneaus and P. ninae from the Western Anatolia. Second clade indicated that P. borysthenicus consists of Black sea and Marmara Sea drainages and northern of Aegean Sea drainages. Haplotype network analysis and phylogeographic inference show that Petroleuciscus genus was monophyletic in Turkey.
Destekleyen Kurum
Recep Tayyip Erdogan University Scientific Research Project Coordination Unit
Proje Numarası
FBA-2018-773
Teşekkür
I am pleased to thank Dr. Davut Turan and Dr. Cüneyt Kaya for providing samples. I also thank Dr. Yusuf Bektaş for genetic analysis, Dr. İsmail Aksu for laboratory studies, and Dr. Esra Bayçelebi for drawing the map. This study was supported by the Scientific Research Project Coordination Unit of Recep Tayyip Erdogan University (Project No: FBA-2018-773).
Kaynakça
- Aksu, I., & Bektaş, Y. (2019). Mitochondrial phylogeny and biogeography of the genus Gobio (Teleostei: Cyprinidae) in Turkey. Zoology in the Middle East, 65(2), 128–141. https://doi.org/10.1080/09397140.2019.1586126
- Bandelt, H. J., Forster, P., & Röhl, A. (1999). Median-joining networks for inferring intraspecific phylogenies. Molecular Biology and Evolution, 16(1), 37–48. https://doi.org/10.1093/oxfordjournals.molbev.a026036
- Battalgil, F. (1940). Yeni bir Cyprinid nev’i. İstanbul Üniv. Fen Fak. Mec., Serie B(5), 74–77.
- Bektas, Y., Aksu, I., Kaya, C., Baycelebi, E., Atasaral, S., Ekmekci, F. G., & Turan, D. (2019). Phylogeny and phylogeography of the genus Alburnoides (Teleostei, Cyprinidae) in Turkey based on mitochondrial DNA sequences. Mitochondrial DNA Part A: DNA Mapping, Sequencing, and Analysis, 30(7), 794–805. https://doi.org/10.1080/24701394.2019.1664493
- Bektas, Y., Turan, D., Aksu, I., Ciftci, Y., Eroglu, O., Kalayci, G., & Belduz, A. O. (2017). Molecular phylogeny of the genus Capoeta (Teleostei: Cyprinidae) in Anatolia, Turkey. Biochemical Systematics and Ecology, 70, 80–94. https://doi.org/10.1016/j.bse.2016.11.005
- Bogutskaya, N. G. (1996). Contribution to the knowledge of leuciscine fishes of Asia Minor. Part 1. Morphology and taxonomic relationships of Leuciscus borysthenicus (Kessler, 1859), Leuciscus smyrnaeus Boulenger, 1896 and Ladigesocypris ghigii (Gianferrari, 1927) (Cyprinidae, Pis. Publ. Espec. Ins. Esp. Oceanogr, 21, 25–44.
- Bogutskaya, N. G. (2002). Petroleusciscus. a new genus for the Leuciscus borysthenicus species group (Teleostei: Cyprinidae). Zoosyst. Rossica, 11(1), 235–237.
- Buj, I., Šanda, R., Zogaris, S., Freyhof, J., Geiger, M. F., & Vukić, J. (2019). Cryptic diversity in Telestes pleurobipunctatus (Actinopterygii; Leuciscidae) as a consequence of historical biogeography in the Ionian Freshwater Ecoregion (Greece, Albania). Hydrobiologia, 835(1), 147–163. https://doi.org/10.1007/s10750-019-3935-6
- Doadrio, I., & Carmona, J. A. (1998). Genetic divergence in Greek populations of the genus Leuciscus and its evolutionary and biogeographical implications. Journal of Fish Biology, 53(3), 591–613. https://doi.org/10.1111/j.1095-8649.1998.tb01004.x
- Drummond, A. J., Suchard, M. A., Xie, D., & Rambaut, A. (2012). Bayesian phylogenetics with BEAUti and the BEAST 1.7. Molecular Biology and Evolution. https://doi.org/10.1093/molbev/mss075
- Durand, J. D., Tsigenopoulos, C. S., Ünlü, E., & Berrebi, P. (2002). Phylogeny and biogeography of the family cyprinidae in the Middle East inferred from cytochrome b DNA - Evolutionary significance of this region. Molecular Phylogenetics and Evolution, 22(1), 91–100. https://doi.org/10.1006/mpev.2001.1040
- Durand, J. D., Unlu, E., Doadrio, I., Pipoyan, S., & Templeton, A. R. (2000). Origin, radiation, dispersion and allopatric hybridization in the chub Leuciscus cephalus. Proceedings of the Royal Society B: Biological Sciences, 267(1453), 1687–1697. https://doi.org/10.1098/rspb.2000.1196
- Excoffier, L., & Lischer, H. E. L. (2010). Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources, 10(3), 564–567. https://doi.org/10.1111/j.1755-0998.2010.02847.x
- Freyhof, J., Kaya, C., Bayçelebi, E., Geiger, M., & Turan, D. (2018). Generic assignment of Leuciscus kurui Bogutskaya from the upper Tigris drainage, and a replacement name for Alburnus kurui Mangit & Yerli (Teleostei: Leuciscidae). Zootaxa, 4410(1), 113–135. https://doi.org/10.11646/zootaxa.4410.1.6
- Froese, R., & Pauly, D. (2018). FishBase. 2018. World Wide Web Electronic Publication. Available at: Http://Www. Fishbase. Org (Accessed on 8 January 2018).
- Geiger, M. F., Herder, F., Monaghan, M. T., Almada, V., Barbieri, R., Bariche, M., Berrebi, P., Bohlen, J., Casal-Lopez, M., Delmastro, G. B., Denys, G. P. J., Dettai, A., Doadrio, I., Kalogianni, E., Kärst, H., Kottelat, M., Kovačić, M., Laporte, M., Lorenzoni, M., … Freyhof, J. (2014). Spatial heterogeneity in the mediterranean biodiversity hotspot affects barcoding accuracy of its freshwater fishes. Molecular Ecology Resources, 14(6), 1210–1221. https://doi.org/10.1111/1755-0998.12257
- Hall, T. A. (1999). BioEdit a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.
- Hasegawa, M., Kishino, H., & Yano, T. aki. (1985). Dating of the human-ape splitting by a molecular clock of mitochondrial DNA. Journal of Molecular Evolution, 22(2), 160–174. https://doi.org/10.1007/BF02101694
- Hrbek, T., Stölting, K. N., Bardakci, F., Küçük, F., Wildekamp, R. H., & Meyer, A. (2004). Plate tectonics and biogeographical patterns of the Pseudophoxinus (Pisces: Cypriniformes) species complex of central Anatolia, Turkey. Molecular Phylogenetics and Evolution, 32(1), 297–308. https://doi.org/10.1016/j.ympev.2003.12.017
- Kimura, M. (1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16(2), 111–120. https://doi.org/10.1007/BF01731581
- Kotlík, P., Bogutskaya, N. G., & Ekmekçi, F. G. (2004). Circum Black Sea phylogeography of Barbus freshwater fishes: Divergence in the Pontic glacial refugium. Molecular Ecology, 13(1), 87–95. https://doi.org/10.1046/j.1365-294X.2003.02021.x
- Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6), 1547–1549. https://doi.org/10.1093/molbev/msy096
- Levin, B. A., Freyhof, J., Lajbner, Z., Perea, S., Abdoli, A., Gaffaroĝlu, M., Özuluĝ, M., Rubenyan, H. R., Salnikov, V. B., & Doadrio, I. (2012). Phylogenetic relationships of the algae scraping cyprinid genus Capoeta (Teleostei: Cyprinidae). Molecular Phylogenetics and Evolution, 62(1), 542–549. https://doi.org/10.1016/j.ympev.2011.09.004
- Levin, B. A., Gandlin, A. A., Simonov, E. S., Levina, M. A., Barmintseva, A. E., Japoshvili, B., Mugue, N. S., Mumladze, L., Mustafayev, N. J., Pashkov, A. N., Roubenyan, H. R., Shapovalov, M. I., & Doadrio, I. (2019). Phylogeny, phylogeography and hybridization of Caucasian barbels of the genus Barbus (Actinopterygii, Cyprinidae). Molecular Phylogenetics and Evolution, 135, 31–44. https://doi.org/10.1016/j.ympev.2019.02.025
- Levy, A., Doadrio, I., & Almada, V. C. (2009). Historical biogeography of European leuciscins (Cyprinidae): Evaluating the Lago Mare dispersal hypothesis. Journal of Biogeography, 36(1), 55–65. https://doi.org/10.1111/j.1365-2699.2008.01969.x
- Perea, S., Böhme, M., Zupančič, P., Freyhof, J., Šanda, R., Özulu, M., Abdoli, A., & Doadrio, I. (2010). Phylogenetic relationships and biogeographical patterns in Circum-Mediterranean subfamily Leuciscinae (Teleostei, Cyprinidae) inferred from both mitochondrial and nuclear data. BMC Evolutionary Biology, 10(1). https://doi.org/10.1186/1471-2148-10-265
- Popov, S., Rögl, F., Rozanov, A., Steininger, F., Shcherba, I., & Kovac, M. (2004). Lithological-paleogeographic maps of Paratethys. 10 maps Late Eocene to Pliocene. In CFS Courier Forschungsinstitut Senckenberg.
- Por, D. F. (1989). The legacy of Tethys. https://doi.org/10.1007/978-94-009-0937-3
- Posada, D. (2008). jModelTest: Phylogenetic model averaging. Molecular Biology and Evolution, 25(7), 1253–1256. https://doi.org/10.1093/molbev/msn083
- Ronquist, F., & Huelsenbeck, J. P. (2003). MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19(12), 1572–1574. https://doi.org/10.1093/bioinformatics/btg180
- Rozas, J., Ferrer-Mata, A., Sanchez-DelBarrio, J. C., Guirao-Rico, S., Librado, P., Ramos-Onsins, S. E., & Sanchez-Gracia, A. (2017). DnaSP 6: DNA sequence polymorphism analysis of large data sets. Molecular Biology and Evolution, 34(12), 3299–3302. https://doi.org/10.1093/molbev/msx248
- Sanjur, O. I., Carmona, J. A., & Doadrio, I. (2003). Evolutionary and biogeographical patterns within Iberian populations of the genus Squalius inferred from molecular data. Molecular Phylogenetics and Evolution. https://doi.org/10.1016/S1055-7903(03)00088-5
- Schönhuth, S., Vukić, J., Šanda, R., Yang, L., & Mayden, R. L. (2018). Phylogenetic relationships and classification of the Holarctic family Leuciscidae (Cypriniformes: Cyprinoidei). Molecular Phylogenetics and Evolution, 127, 781–799. https://doi.org/10.1016/j.ympev.2018.06.026
- Swofford, D. L. (2003). PAUP*: phylogenetic analysis using parsimony, version 4.0b10. In Sinauer Associates. https://doi.org/citeulike-article-id:2345226
- Tamura K, & Nei, M. (1993). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution, 10(3), 512–526. https://doi.org/10.1093/oxfordjournals.molbev.a040023
- Thompson, J. D., Higgins, D. G., & Gibson, T. J. (1994). CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22(22), 4673–4680. https://doi.org/10.1093/nar/22.22.4673
- Tsigenopoulos, C. S., Kotlík, P., & Berrebi, P. (2002). Biogeography and pattern of gene flow among Barbus species (Teleostei: Cyprinidae) inhabiting the Italian Peninsula and neighbouring Adriatic drainages as revealed by allozyme and mitochondrial sequence data. Biological Journal of the Linnean Society, 75(1), 83–99. https://doi.org/10.1046/j.1095-8312.2002.00007.x
- Turan, D., Kalayci, G., Kaya, C., Bektaş, Y., & Küçük, F. (2018). A new species of Petroleuciscus (Teleostei: Cyprinidae) from the Büyük Menderes River, southwestern Anatolia, Turkey. Journal of Fish Biology, 92(4), 875–887. https://doi.org/10.1111/jfb.13525
- Turan, D., Kaya, C., Kalayci, G., Bayçelebi, E., & Aksu, İ. (2019). Oxynoemacheilus cemali, a new species of stone loach (Teleostei: Nemacheilidae) from the Çoruh River drainage, Turkey. Journal of Fish Biology, 94(3), 458–468. https://doi.org/10.1111/jfb.13909
- Zardoya, R., & Doadrio, I. (1999). Molecular evidence on the evolutionary and biogeographical patterns of European cyprinids. Journal of Molecular Evolution, 49(2), 227–237. https://doi.org/10.1007/PL00006545
- Zardoya, R., Economidis, P. S., & Doadrio, I. (1999). Phylogenetic Relationships of Greek Cyprinidae: Molecular Evidence for at Least Two Origins of the Greek Cyprinid Fauna. Molecular Phylogenetics and Evolution, 13(1), 122–131. https://doi.org/https://doi.org/10.1006/mpev.1999.0630
Türkiye'deki Petroleuciscus (Teleostei: Leuciscidae) Türlerinin Moleküler Filogenisi ve Tarihsel Biyocoğrafyası
Yıl 2022,
Cilt: 7 Sayı: 1, 88 - 95, 31.03.2022
Gökhan Kalaycı
Öz
Bu çalışmada, Türkiye'deki Petroleuciscus türlerinin filogenetik ilişkileri mitokondriyal sitokrom b (cyt b) (1140 bp) geni kullanılarak araştırılmıştır. Bu amaçla, 13 populasyondan 84 örneğin cyt b geni dizilenmiştir. Genetik analizler sonucunda 21 haplotip tanımlandı ve haplotip çeşitliliği Hd: 0.921±0.011 olarak hesaplanmıştır. Petroleuciscus türlerinin filogenetik analizinin sonucunda ise, iki klad ve dört haplogrup varlığı ortaya çıkmaktadır. Birinci klad, Batı Anadolu'dan Petroleuciscus smyrneaus ve P. ninae' den oluşmaktadır. İkinci klad ise, P. borysthenicus'un Karadeniz ve Marmara Denizi havzasından ve Ege Denizi'nin kuzey sularından oluştuğunu ortaya çıkarmaktadır. Haplotip ağ analizi ve filocoğrafik çıkarım, Türkiye'de Petroleuciscus cinsinin monofiletik olduğunu göstermektedir.
Proje Numarası
FBA-2018-773
Kaynakça
- Aksu, I., & Bektaş, Y. (2019). Mitochondrial phylogeny and biogeography of the genus Gobio (Teleostei: Cyprinidae) in Turkey. Zoology in the Middle East, 65(2), 128–141. https://doi.org/10.1080/09397140.2019.1586126
- Bandelt, H. J., Forster, P., & Röhl, A. (1999). Median-joining networks for inferring intraspecific phylogenies. Molecular Biology and Evolution, 16(1), 37–48. https://doi.org/10.1093/oxfordjournals.molbev.a026036
- Battalgil, F. (1940). Yeni bir Cyprinid nev’i. İstanbul Üniv. Fen Fak. Mec., Serie B(5), 74–77.
- Bektas, Y., Aksu, I., Kaya, C., Baycelebi, E., Atasaral, S., Ekmekci, F. G., & Turan, D. (2019). Phylogeny and phylogeography of the genus Alburnoides (Teleostei, Cyprinidae) in Turkey based on mitochondrial DNA sequences. Mitochondrial DNA Part A: DNA Mapping, Sequencing, and Analysis, 30(7), 794–805. https://doi.org/10.1080/24701394.2019.1664493
- Bektas, Y., Turan, D., Aksu, I., Ciftci, Y., Eroglu, O., Kalayci, G., & Belduz, A. O. (2017). Molecular phylogeny of the genus Capoeta (Teleostei: Cyprinidae) in Anatolia, Turkey. Biochemical Systematics and Ecology, 70, 80–94. https://doi.org/10.1016/j.bse.2016.11.005
- Bogutskaya, N. G. (1996). Contribution to the knowledge of leuciscine fishes of Asia Minor. Part 1. Morphology and taxonomic relationships of Leuciscus borysthenicus (Kessler, 1859), Leuciscus smyrnaeus Boulenger, 1896 and Ladigesocypris ghigii (Gianferrari, 1927) (Cyprinidae, Pis. Publ. Espec. Ins. Esp. Oceanogr, 21, 25–44.
- Bogutskaya, N. G. (2002). Petroleusciscus. a new genus for the Leuciscus borysthenicus species group (Teleostei: Cyprinidae). Zoosyst. Rossica, 11(1), 235–237.
- Buj, I., Šanda, R., Zogaris, S., Freyhof, J., Geiger, M. F., & Vukić, J. (2019). Cryptic diversity in Telestes pleurobipunctatus (Actinopterygii; Leuciscidae) as a consequence of historical biogeography in the Ionian Freshwater Ecoregion (Greece, Albania). Hydrobiologia, 835(1), 147–163. https://doi.org/10.1007/s10750-019-3935-6
- Doadrio, I., & Carmona, J. A. (1998). Genetic divergence in Greek populations of the genus Leuciscus and its evolutionary and biogeographical implications. Journal of Fish Biology, 53(3), 591–613. https://doi.org/10.1111/j.1095-8649.1998.tb01004.x
- Drummond, A. J., Suchard, M. A., Xie, D., & Rambaut, A. (2012). Bayesian phylogenetics with BEAUti and the BEAST 1.7. Molecular Biology and Evolution. https://doi.org/10.1093/molbev/mss075
- Durand, J. D., Tsigenopoulos, C. S., Ünlü, E., & Berrebi, P. (2002). Phylogeny and biogeography of the family cyprinidae in the Middle East inferred from cytochrome b DNA - Evolutionary significance of this region. Molecular Phylogenetics and Evolution, 22(1), 91–100. https://doi.org/10.1006/mpev.2001.1040
- Durand, J. D., Unlu, E., Doadrio, I., Pipoyan, S., & Templeton, A. R. (2000). Origin, radiation, dispersion and allopatric hybridization in the chub Leuciscus cephalus. Proceedings of the Royal Society B: Biological Sciences, 267(1453), 1687–1697. https://doi.org/10.1098/rspb.2000.1196
- Excoffier, L., & Lischer, H. E. L. (2010). Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources, 10(3), 564–567. https://doi.org/10.1111/j.1755-0998.2010.02847.x
- Freyhof, J., Kaya, C., Bayçelebi, E., Geiger, M., & Turan, D. (2018). Generic assignment of Leuciscus kurui Bogutskaya from the upper Tigris drainage, and a replacement name for Alburnus kurui Mangit & Yerli (Teleostei: Leuciscidae). Zootaxa, 4410(1), 113–135. https://doi.org/10.11646/zootaxa.4410.1.6
- Froese, R., & Pauly, D. (2018). FishBase. 2018. World Wide Web Electronic Publication. Available at: Http://Www. Fishbase. Org (Accessed on 8 January 2018).
- Geiger, M. F., Herder, F., Monaghan, M. T., Almada, V., Barbieri, R., Bariche, M., Berrebi, P., Bohlen, J., Casal-Lopez, M., Delmastro, G. B., Denys, G. P. J., Dettai, A., Doadrio, I., Kalogianni, E., Kärst, H., Kottelat, M., Kovačić, M., Laporte, M., Lorenzoni, M., … Freyhof, J. (2014). Spatial heterogeneity in the mediterranean biodiversity hotspot affects barcoding accuracy of its freshwater fishes. Molecular Ecology Resources, 14(6), 1210–1221. https://doi.org/10.1111/1755-0998.12257
- Hall, T. A. (1999). BioEdit a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.
- Hasegawa, M., Kishino, H., & Yano, T. aki. (1985). Dating of the human-ape splitting by a molecular clock of mitochondrial DNA. Journal of Molecular Evolution, 22(2), 160–174. https://doi.org/10.1007/BF02101694
- Hrbek, T., Stölting, K. N., Bardakci, F., Küçük, F., Wildekamp, R. H., & Meyer, A. (2004). Plate tectonics and biogeographical patterns of the Pseudophoxinus (Pisces: Cypriniformes) species complex of central Anatolia, Turkey. Molecular Phylogenetics and Evolution, 32(1), 297–308. https://doi.org/10.1016/j.ympev.2003.12.017
- Kimura, M. (1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16(2), 111–120. https://doi.org/10.1007/BF01731581
- Kotlík, P., Bogutskaya, N. G., & Ekmekçi, F. G. (2004). Circum Black Sea phylogeography of Barbus freshwater fishes: Divergence in the Pontic glacial refugium. Molecular Ecology, 13(1), 87–95. https://doi.org/10.1046/j.1365-294X.2003.02021.x
- Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6), 1547–1549. https://doi.org/10.1093/molbev/msy096
- Levin, B. A., Freyhof, J., Lajbner, Z., Perea, S., Abdoli, A., Gaffaroĝlu, M., Özuluĝ, M., Rubenyan, H. R., Salnikov, V. B., & Doadrio, I. (2012). Phylogenetic relationships of the algae scraping cyprinid genus Capoeta (Teleostei: Cyprinidae). Molecular Phylogenetics and Evolution, 62(1), 542–549. https://doi.org/10.1016/j.ympev.2011.09.004
- Levin, B. A., Gandlin, A. A., Simonov, E. S., Levina, M. A., Barmintseva, A. E., Japoshvili, B., Mugue, N. S., Mumladze, L., Mustafayev, N. J., Pashkov, A. N., Roubenyan, H. R., Shapovalov, M. I., & Doadrio, I. (2019). Phylogeny, phylogeography and hybridization of Caucasian barbels of the genus Barbus (Actinopterygii, Cyprinidae). Molecular Phylogenetics and Evolution, 135, 31–44. https://doi.org/10.1016/j.ympev.2019.02.025
- Levy, A., Doadrio, I., & Almada, V. C. (2009). Historical biogeography of European leuciscins (Cyprinidae): Evaluating the Lago Mare dispersal hypothesis. Journal of Biogeography, 36(1), 55–65. https://doi.org/10.1111/j.1365-2699.2008.01969.x
- Perea, S., Böhme, M., Zupančič, P., Freyhof, J., Šanda, R., Özulu, M., Abdoli, A., & Doadrio, I. (2010). Phylogenetic relationships and biogeographical patterns in Circum-Mediterranean subfamily Leuciscinae (Teleostei, Cyprinidae) inferred from both mitochondrial and nuclear data. BMC Evolutionary Biology, 10(1). https://doi.org/10.1186/1471-2148-10-265
- Popov, S., Rögl, F., Rozanov, A., Steininger, F., Shcherba, I., & Kovac, M. (2004). Lithological-paleogeographic maps of Paratethys. 10 maps Late Eocene to Pliocene. In CFS Courier Forschungsinstitut Senckenberg.
- Por, D. F. (1989). The legacy of Tethys. https://doi.org/10.1007/978-94-009-0937-3
- Posada, D. (2008). jModelTest: Phylogenetic model averaging. Molecular Biology and Evolution, 25(7), 1253–1256. https://doi.org/10.1093/molbev/msn083
- Ronquist, F., & Huelsenbeck, J. P. (2003). MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19(12), 1572–1574. https://doi.org/10.1093/bioinformatics/btg180
- Rozas, J., Ferrer-Mata, A., Sanchez-DelBarrio, J. C., Guirao-Rico, S., Librado, P., Ramos-Onsins, S. E., & Sanchez-Gracia, A. (2017). DnaSP 6: DNA sequence polymorphism analysis of large data sets. Molecular Biology and Evolution, 34(12), 3299–3302. https://doi.org/10.1093/molbev/msx248
- Sanjur, O. I., Carmona, J. A., & Doadrio, I. (2003). Evolutionary and biogeographical patterns within Iberian populations of the genus Squalius inferred from molecular data. Molecular Phylogenetics and Evolution. https://doi.org/10.1016/S1055-7903(03)00088-5
- Schönhuth, S., Vukić, J., Šanda, R., Yang, L., & Mayden, R. L. (2018). Phylogenetic relationships and classification of the Holarctic family Leuciscidae (Cypriniformes: Cyprinoidei). Molecular Phylogenetics and Evolution, 127, 781–799. https://doi.org/10.1016/j.ympev.2018.06.026
- Swofford, D. L. (2003). PAUP*: phylogenetic analysis using parsimony, version 4.0b10. In Sinauer Associates. https://doi.org/citeulike-article-id:2345226
- Tamura K, & Nei, M. (1993). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution, 10(3), 512–526. https://doi.org/10.1093/oxfordjournals.molbev.a040023
- Thompson, J. D., Higgins, D. G., & Gibson, T. J. (1994). CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22(22), 4673–4680. https://doi.org/10.1093/nar/22.22.4673
- Tsigenopoulos, C. S., Kotlík, P., & Berrebi, P. (2002). Biogeography and pattern of gene flow among Barbus species (Teleostei: Cyprinidae) inhabiting the Italian Peninsula and neighbouring Adriatic drainages as revealed by allozyme and mitochondrial sequence data. Biological Journal of the Linnean Society, 75(1), 83–99. https://doi.org/10.1046/j.1095-8312.2002.00007.x
- Turan, D., Kalayci, G., Kaya, C., Bektaş, Y., & Küçük, F. (2018). A new species of Petroleuciscus (Teleostei: Cyprinidae) from the Büyük Menderes River, southwestern Anatolia, Turkey. Journal of Fish Biology, 92(4), 875–887. https://doi.org/10.1111/jfb.13525
- Turan, D., Kaya, C., Kalayci, G., Bayçelebi, E., & Aksu, İ. (2019). Oxynoemacheilus cemali, a new species of stone loach (Teleostei: Nemacheilidae) from the Çoruh River drainage, Turkey. Journal of Fish Biology, 94(3), 458–468. https://doi.org/10.1111/jfb.13909
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