Year 2019, Volume 5, Issue 1, Pages 1157 - 1167 2019-04-15

RT-PCR yöntemi kullanılarak Dejeneratif Lomber Disk Herniasyonunda İleri Düzey Protrüde ve Ekstrüde Nükleus Pulposusun Antioksidan mRNA ekspresyon düzeylerinin Karşılaştırılması
Comparison of Antioxidant mRNA expression levels of advanced Protruded and Extruded Nucleus Pulposus in Degenerative Lumbar Disc Herniations using the RT-PCR method

Şeyho Cem YÜCETAŞ [1] , Necati Üçler [2]

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Amaç. Bu çalışmanın amacı, Real Time-Polimeraz Zincir Reaksiyonu (RT-PCR) yöntemi ile lumbar posterior longitudinal ligamentte ileri düzey protrude ve ekstrüde lomber disk hernilerinde (LDH) nükleus pulposusun antioksidan düzeylerini karşılaştırmaktır.

Metot: Bu çalışma, Ocak 2013 ile Mayıs 2016 arasında izolasyon ve RT-PCR yöntemi ile ileri düzey protrüde (n = 20) ve ekstrüde (n = 20) LDH ile TRI-Reagent® ve Ribonükleik asit (RNA) ile ameliyat edilen toplam 40 hastanın disk bölümlerinin karşılaştırılması ile yapıldı. Çalışmaya, mikrodisektomi cerrahisi uygulanan lomber disk herniasyonu tanısı alan hastalar dahil edildi. Sıkışmaya neden olan disk materyalinin antioksidan seviyeleri, RT-PCR yöntemi kullanılarak tespit edildi ve genlerin ekspresyon seviyeleri belirlendi.

Bulgular: Protrude ve ekstrüde disk hernisi nedeniyle ameliyat edilen toplam 40 hastanın disk materyallerinin antioksidan düzeyleri RT-PCR yöntemi ile belirlendi. Çalışmadaki 25 kadın hastanın yaş ortalaması 36.4 ve 15 erkek hastanın yaş ortalaması 39.26 olduğu belirlendi. Toplamda hastaların 20'sinde MacNab grade 2 (protruded) ve 20'sinde MacNab grade 3 (ekstrüde) disk fıtığı vardı. Ekspresyon sonucunda  protrüde LDH materyallerinin ekstrüde LDH materyallerinden daha düşük olduğu bulundu.

Sonuç: Ekstrüde LDH'si olan hastalarda TGF-β, FGF-β, IGF-1, NGF, MMP-3 ve GAPDH mRNA'nın anlamlı antioksidan moleküllerinin ekspresyon seviyeleri, protrüde LDH hastalara göre anlamlı olarak daha yüksek bulunmasına rağmen, bu düzeylerin diskojenik ağrı ve postoperative  klinik iyileşme açısından ayırt edici özellik olarak belirlenemedi.

Purpose: The aim of the present study is to compare the antioxidant levels of nucleus pulposus in advanced protruded and extruded lumbar disc hernias (LDH) with a steady lumbar posterior longitudinal ligament with the Real Time-Polymerase Chain Reaction (RT-PCR) method.

Method: This study was conducted with the comparison of the disc sections of a total of 40 patients who underwent an operation due to advanced protruded (n=20) and extruded (n=20) LDH with the TRI-Reagent® and Ribonucleic acid (RNA) isolation and the RT-PCR method between January 2013 and May 2016. The study included patients diagnosed with lumbar disc herniation who were applied with microdiscectomy surgery. The antioxidant levels of the disc material, which caused compression because it was highly protrudedorextruded, were detected using the RT-PCR method,and the expression levels of the genes were determined.

Results: The antioxidant levels of the disc materials of a total of 40 patients, who underwent an operation due to protruded and extruded disc hernia, were determined with the RT-PCR method. The patients were found 25 females with a mean age of 36.4 years and 15 males with a mean age of 39.26 years. Of the total patients, 20 had MacNab grade 2 (protruded) and 20 had  MacNab grade 3 (extruded) disc hernias. The expressions of protruded LDH materials were found to be lower than those of extruded LDH materials.

Conclusion: Although the expression levels of the significant antioxidant molecules of TGF-β, FGF-β, IGF-1, NGF, MMP-3, and GAPDH mRNA, in patients with extruded LDH were significantly higher compared to those with protruded LDH, no distinctive features of these levels could be determined in terms of discogenic pain and postoperative clinical improvement.

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Primary Language en
Subjects Health Care Sciences and Services
Journal Section Araştırma/Research
Authors

Orcid: 0000-0002-2891-1805
Author: Şeyho Cem YÜCETAŞ
Country: Turkey


Orcid: 0000-0002-0561-5819
Author: Necati Üçler

Dates

Publication Date: April 15, 2019

Bibtex @research article { adiyamansaglik486946, journal = {Adıyaman Üniversitesi Sağlık Bilimleri Dergisi}, issn = {}, eissn = {2458-9179}, address = {Adıyaman University}, year = {2019}, volume = {5}, pages = {1157 - 1167}, doi = {10.30569/adiyamansaglik.486946}, title = {Comparison of Antioxidant mRNA expression levels of advanced Protruded and Extruded Nucleus Pulposus in Degenerative Lumbar Disc Herniations using the RT-PCR method}, key = {cite}, author = {YÜCETAŞ, Şeyho Cem and Üçler, Necati} }
APA YÜCETAŞ, Ş , Üçler, N . (2019). Comparison of Antioxidant mRNA expression levels of advanced Protruded and Extruded Nucleus Pulposus in Degenerative Lumbar Disc Herniations using the RT-PCR method. Adıyaman Üniversitesi Sağlık Bilimleri Dergisi, 5 (1), 1157-1167. DOI: 10.30569/adiyamansaglik.486946
MLA YÜCETAŞ, Ş , Üçler, N . "Comparison of Antioxidant mRNA expression levels of advanced Protruded and Extruded Nucleus Pulposus in Degenerative Lumbar Disc Herniations using the RT-PCR method". Adıyaman Üniversitesi Sağlık Bilimleri Dergisi 5 (2019): 1157-1167 <http://dergipark.org.tr/adiyamansaglik/issue/43834/486946>
Chicago YÜCETAŞ, Ş , Üçler, N . "Comparison of Antioxidant mRNA expression levels of advanced Protruded and Extruded Nucleus Pulposus in Degenerative Lumbar Disc Herniations using the RT-PCR method". Adıyaman Üniversitesi Sağlık Bilimleri Dergisi 5 (2019): 1157-1167
RIS TY - JOUR T1 - Comparison of Antioxidant mRNA expression levels of advanced Protruded and Extruded Nucleus Pulposus in Degenerative Lumbar Disc Herniations using the RT-PCR method AU - Şeyho Cem YÜCETAŞ , Necati Üçler Y1 - 2019 PY - 2019 N1 - doi: 10.30569/adiyamansaglik.486946 DO - 10.30569/adiyamansaglik.486946 T2 - Adıyaman Üniversitesi Sağlık Bilimleri Dergisi JF - Journal JO - JOR SP - 1157 EP - 1167 VL - 5 IS - 1 SN - -2458-9179 M3 - doi: 10.30569/adiyamansaglik.486946 UR - https://doi.org/10.30569/adiyamansaglik.486946 Y2 - 2018 ER -
EndNote %0 Health Sciences Journal of Adıyaman University Comparison of Antioxidant mRNA expression levels of advanced Protruded and Extruded Nucleus Pulposus in Degenerative Lumbar Disc Herniations using the RT-PCR method %A Şeyho Cem YÜCETAŞ , Necati Üçler %T Comparison of Antioxidant mRNA expression levels of advanced Protruded and Extruded Nucleus Pulposus in Degenerative Lumbar Disc Herniations using the RT-PCR method %D 2019 %J Adıyaman Üniversitesi Sağlık Bilimleri Dergisi %P -2458-9179 %V 5 %N 1 %R doi: 10.30569/adiyamansaglik.486946 %U 10.30569/adiyamansaglik.486946
ISNAD YÜCETAŞ, Şeyho Cem , Üçler, Necati . "Comparison of Antioxidant mRNA expression levels of advanced Protruded and Extruded Nucleus Pulposus in Degenerative Lumbar Disc Herniations using the RT-PCR method". Adıyaman Üniversitesi Sağlık Bilimleri Dergisi 5 / 1 (April 2019): 1157-1167. https://doi.org/10.30569/adiyamansaglik.486946
AMA YÜCETAŞ Ş , Üçler N . Comparison of Antioxidant mRNA expression levels of advanced Protruded and Extruded Nucleus Pulposus in Degenerative Lumbar Disc Herniations using the RT-PCR method. Adıyaman Üniversitesi Sağlık Bilimleri Dergisi. 2019; 5(1): 1157-1167.
Vancouver YÜCETAŞ Ş , Üçler N . Comparison of Antioxidant mRNA expression levels of advanced Protruded and Extruded Nucleus Pulposus in Degenerative Lumbar Disc Herniations using the RT-PCR method. Adıyaman Üniversitesi Sağlık Bilimleri Dergisi. 2019; 5(1): 1167-1157.