Year 2018, Volume 7, Issue 2, Pages 179 - 184 2018-12-26

Effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in Echinacea purpurea L. callus cultures

Muhammed Akif AÇIKGÖZ [1] , Şevket Metin KARA [2] , Ebru BATI AY [3] , Sedanur ODABAŞ [4]

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Light is not only effective in photosynthesis, growth and development, but also play an important role in biosynthesis of primary and secondary metabolites. The present research was aimed to determine effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in cell suspension cultures of Echinacea purpurea L. Stem explants derived from sterile plants were subjected to callus culture in a B5 media supplemented with 1.0 mg/L BAP and 2.0 mg/L NAA. Afterward, 8-day cell cultures were incubated in light and dark environments and cell harvesting, with a three-day interval, was carried out five times. The contents of alkamide, caffeic acid derivatives and echinacoside were determined using HPLC. Alkamide, caftaric acid and echinacoside content increased regularly with light application. Light application increased alkamide content by 57%, as an average, compared to darkness. The cell cultures exposed to 12 days of incubation in light produced the highest alkamide content, with a 2.11-fold increase, compared to dark. Subjecting the cell cultures to light for 12 days resulted in 70% and 63% increases in caftaric acid and echinacoside. This study showed that light application in Echinacea purpurea L. cell suspension culture had a great potential for increasing some important phytochemicals.
Caftaric acid, elicitor, purple coneflower, phytochemical compounds, secondary metabolites
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Primary Language en
Journal Section Makaleler
Authors

Author: Muhammed Akif AÇIKGÖZ

Author: Şevket Metin KARA (Primary Author)

Author: Ebru BATI AY

Author: Sedanur ODABAŞ

Bibtex @research article { azd476349, journal = {Akademik Ziraat Dergisi}, issn = {2147-6403}, eissn = {2618-5881}, address = {Ordu University}, year = {2018}, volume = {7}, pages = {179 - 184}, doi = {10.29278/azd.476349}, title = {Effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in Echinacea purpurea L. callus cultures}, key = {cite}, author = {AÇIKGÖZ, Muhammed Akif and KARA, Şevket Metin and BATI AY, Ebru and ODABAŞ, Sedanur} }
APA AÇIKGÖZ, M , KARA, Ş , BATI AY, E , ODABAŞ, S . (2018). Effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in Echinacea purpurea L. callus cultures. Akademik Ziraat Dergisi, 7 (2), 179-184. DOI: 10.29278/azd.476349
MLA AÇIKGÖZ, M , KARA, Ş , BATI AY, E , ODABAŞ, S . "Effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in Echinacea purpurea L. callus cultures". Akademik Ziraat Dergisi 7 (2018): 179-184 <http://dergipark.org.tr/azd/issue/40046/476349>
Chicago AÇIKGÖZ, M , KARA, Ş , BATI AY, E , ODABAŞ, S . "Effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in Echinacea purpurea L. callus cultures". Akademik Ziraat Dergisi 7 (2018): 179-184
RIS TY - JOUR T1 - Effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in Echinacea purpurea L. callus cultures AU - Muhammed Akif AÇIKGÖZ , Şevket Metin KARA , Ebru BATI AY , Sedanur ODABAŞ Y1 - 2018 PY - 2018 N1 - doi: 10.29278/azd.476349 DO - 10.29278/azd.476349 T2 - Akademik Ziraat Dergisi JF - Journal JO - JOR SP - 179 EP - 184 VL - 7 IS - 2 SN - 2147-6403-2618-5881 M3 - doi: 10.29278/azd.476349 UR - https://doi.org/10.29278/azd.476349 Y2 - 2018 ER -
EndNote %0 Akademik Ziraat Dergisi Effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in Echinacea purpurea L. callus cultures %A Muhammed Akif AÇIKGÖZ , Şevket Metin KARA , Ebru BATI AY , Sedanur ODABAŞ %T Effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in Echinacea purpurea L. callus cultures %D 2018 %J Akademik Ziraat Dergisi %P 2147-6403-2618-5881 %V 7 %N 2 %R doi: 10.29278/azd.476349 %U 10.29278/azd.476349
ISNAD AÇIKGÖZ, Muhammed Akif , KARA, Şevket Metin , BATI AY, Ebru , ODABAŞ, Sedanur . "Effect of light on biosynthesis of alkamide, caffeic acid derivatives and echinacoside in Echinacea purpurea L. callus cultures". Akademik Ziraat Dergisi 7 / 2 (December 2018): 179-184. https://doi.org/10.29278/azd.476349