Year 2018, Volume 7, Issue 1, Pages 90 - 97 2018-06-29

Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes

Namık KILINÇ [1] , Zuhal ALIM [2] , Bülent ŞENGÜL [3] , Mehmet Mustafa İŞGÖR [4] , Şükrü BEYDEMİR [5]

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Abstract

Carbonic anhydrase enzyme plays a vital role in metabolic events such as acid-base regulation and respiration. In our research, it is tried to determine the inhibitory influences of the cardiovascular therapeutics esmolol hydrochloride, amiodarone hydrochloride and lidocaine hydrochloride on human erythrocytes carbonic anhydrases (hCAI and II). In accordance with this purpose, carbonic anhydrase isoenzymes were purified from human erythrocytes by using affinity chromatography method. Enzyme purity was checked by SDS-PAGE electrophoresis method. After the aldose reductase enzymes were purified, the inhibitory affects of cardiovascular therapeutics on these enzymes, using esterase activity, which is the method of measuring in vitro activity, were examined. The three cardiovascular therapeutics dose-dependently decreased activity of hCAs. IC50 values of amiodarone hydrochloride, esmolol hydrochloride and lidocaine hydrochloride were found to be, respectively, 0.91 mM, 5 mM, 5.8 mM for hCA-I and 0.41 mM, 3.5 mM, and 6.36 mM for hCA-II. Our results proved that, under in vitro conditions, cardiovascular therapeutics significantly inhibit human CA-I and II activities. So, irregular use of these medicines may cause serious adverse effects in terms of human health.

Carbonic Anhydrase, Cardiovascular Therapeutics, Enzyme Inhibition
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Primary Language en
Subjects Science
Journal Section Articles
Authors

Orcid: orcid.org/0000-0002-9102-1370
Author: Namık KILINÇ
Institution: IĞDIR ÜNİVERSİTESİ
Country: Turkey


Author: Zuhal ALIM
Institution: AHİ EVRAN ÜNİVERSİTESİ

Author: Bülent ŞENGÜL
Institution: BAYBURT ÜNİVERSİTESİ

Author: Mehmet Mustafa İŞGÖR
Institution: MUSTAFA KEMAL ÜNİVERSİTESİ

Author: Şükrü BEYDEMİR
Institution: ANADOLU ÜNİVERSİTESİ

Bibtex @research article { bitlisfen345643, journal = {Bitlis Eren Üniversitesi Fen Bilimleri Dergisi}, issn = {2147-3129}, eissn = {2147-3188}, address = {Bitlis Eren University}, year = {2018}, volume = {7}, pages = {90 - 97}, doi = {10.17798/bitlisfen.345643}, title = {Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes}, key = {cite}, author = {KILINÇ, Namık and ALIM, Zuhal and ŞENGÜL, Bülent and İŞGÖR, Mehmet and BEYDEMİR, Şükrü} }
APA KILINÇ, N , ALIM, Z , ŞENGÜL, B , İŞGÖR, M , BEYDEMİR, Ş . (2018). Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, 7 (1), 90-97. DOI: 10.17798/bitlisfen.345643
MLA KILINÇ, N , ALIM, Z , ŞENGÜL, B , İŞGÖR, M , BEYDEMİR, Ş . "Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes". Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 7 (2018): 90-97 <http://dergipark.org.tr/bitlisfen/issue/38033/345643>
Chicago KILINÇ, N , ALIM, Z , ŞENGÜL, B , İŞGÖR, M , BEYDEMİR, Ş . "Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes". Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 7 (2018): 90-97
RIS TY - JOUR T1 - Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes AU - Namık KILINÇ , Zuhal ALIM , Bülent ŞENGÜL , Mehmet Mustafa İŞGÖR , Şükrü BEYDEMİR Y1 - 2018 PY - 2018 N1 - doi: 10.17798/bitlisfen.345643 DO - 10.17798/bitlisfen.345643 T2 - Bitlis Eren Üniversitesi Fen Bilimleri Dergisi JF - Journal JO - JOR SP - 90 EP - 97 VL - 7 IS - 1 SN - 2147-3129-2147-3188 M3 - doi: 10.17798/bitlisfen.345643 UR - https://doi.org/10.17798/bitlisfen.345643 Y2 - 2018 ER -
EndNote %0 Bitlis Eren Üniversitesi Fen Bilimleri Dergisi Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes %A Namık KILINÇ , Zuhal ALIM , Bülent ŞENGÜL , Mehmet Mustafa İŞGÖR , Şükrü BEYDEMİR %T Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes %D 2018 %J Bitlis Eren Üniversitesi Fen Bilimleri Dergisi %P 2147-3129-2147-3188 %V 7 %N 1 %R doi: 10.17798/bitlisfen.345643 %U 10.17798/bitlisfen.345643
ISNAD KILINÇ, Namık , ALIM, Zuhal , ŞENGÜL, Bülent , İŞGÖR, Mehmet , BEYDEMİR, Şükrü . "Evaluation of Inhibition Effects of Some Cardiovascular Therapeutics on Human Erythrocyte Carbonic Anhydrase Isoenzymes". Bitlis Eren Üniversitesi Fen Bilimleri Dergisi 7 / 1 (June 2018): 90-97. https://doi.org/10.17798/bitlisfen.345643