Year 2018, Volume 14, Issue 4, Pages 369 - 372 2018-12-28

In Vitro Propagation of Wetland Medicinal Plant Lythrum salicaria L.

Betül Akın [1] , Burcu Çetin [2] , Nüket Akanıl Bingöl [3]

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The aim of this experiment is to develop a protocol to establish the in vitro propagation potential of Lythrum salicaria L (purple loosestrife). Seeds of L. salicaria were germinated and grown in Murashige Skoog (MS) medium for 30 days. At the end of 30 days, shoot tips of L. salicaria were cultured on MS medium supplemented with different combinations and concentrations of 6-benzylaminopurine (BAP; 1, 2 and 3 mg/L) and 1-naphthaleneacetic acid (NAA; 0, 0.1 and 0.5 mg/L). Regenerated shoots were transferred to MS supplemented with varying concentrations of different auxins: NAA (0.5 and 1 mg/L) and indole-3-butyric acid (IBA; 0.5 and 1.0 mg/L) for rooting. Among the cytokinins investigated, BAP indicated the best response to initiate shoot elongation and multiple shoot formation alone. The highest number of shoot per explant (37±3.38) was obtained in MS medium with 1 mg/L BAP. Although, the highest shoot length (3.1 cm ± 0.48) was found on medium with 1 mg/L BAP+0.5 mg/L NAA, there were no statistically differences among all types of medium. Percentage of rooting, shoot length and root length were altered significantly with different concentrations of NAA and IBA. Thus, shoot length (9.5 cm±0.46), root length (4.1 cm±0.13) and the percentage of rooting (100%) increased significantly with a concentration of 1 mg/L NAA.  It was concluded that MS medium with 1 mg/L NAA has been superior for root development compared to other hormone concentrations.

auxins, cytokinins, purple loosestrife, rooting, tissue culture
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Primary Language en
Subjects Engineering
Published Date 31-12-2018
Journal Section Articles
Authors

Author: Betül Akın
Institution: Dumlupınar University
Country: Turkey


Author: Burcu Çetin (Primary Author)
Country: Turkey


Author: Nüket Akanıl Bingöl
Country: Turkey


Bibtex @research article { cbayarfbe393626, journal = {Celal Bayar University Journal of Science}, issn = {1305-130X}, eissn = {1305-1385}, address = {Celal Bayar University}, year = {2018}, volume = {14}, pages = {369 - 372}, doi = {10.18466/cbayarfbe.393626}, title = {In Vitro Propagation of Wetland Medicinal Plant Lythrum salicaria L.}, key = {cite}, author = {Akın, Betül and Çetin, Burcu and Akanıl Bingöl, Nüket} }
APA Akın, B , Çetin, B , Akanıl Bingöl, N . (2018). In Vitro Propagation of Wetland Medicinal Plant Lythrum salicaria L.. Celal Bayar University Journal of Science, 14 (4), 369-372. DOI: 10.18466/cbayarfbe.393626
MLA Akın, B , Çetin, B , Akanıl Bingöl, N . "In Vitro Propagation of Wetland Medicinal Plant Lythrum salicaria L.". Celal Bayar University Journal of Science 14 (2018): 369-372 <http://dergipark.org.tr/cbayarfbe/issue/41718/393626>
Chicago Akın, B , Çetin, B , Akanıl Bingöl, N . "In Vitro Propagation of Wetland Medicinal Plant Lythrum salicaria L.". Celal Bayar University Journal of Science 14 (2018): 369-372
RIS TY - JOUR T1 - In Vitro Propagation of Wetland Medicinal Plant Lythrum salicaria L. AU - Betül Akın , Burcu Çetin , Nüket Akanıl Bingöl Y1 - 2018 PY - 2018 N1 - doi: 10.18466/cbayarfbe.393626 DO - 10.18466/cbayarfbe.393626 T2 - Celal Bayar University Journal of Science JF - Journal JO - JOR SP - 369 EP - 372 VL - 14 IS - 4 SN - 1305-130X-1305-1385 M3 - doi: 10.18466/cbayarfbe.393626 UR - https://doi.org/10.18466/cbayarfbe.393626 Y2 - 2018 ER -
EndNote %0 Celal Bayar University Journal of Science In Vitro Propagation of Wetland Medicinal Plant Lythrum salicaria L. %A Betül Akın , Burcu Çetin , Nüket Akanıl Bingöl %T In Vitro Propagation of Wetland Medicinal Plant Lythrum salicaria L. %D 2018 %J Celal Bayar University Journal of Science %P 1305-130X-1305-1385 %V 14 %N 4 %R doi: 10.18466/cbayarfbe.393626 %U 10.18466/cbayarfbe.393626
ISNAD Akın, Betül , Çetin, Burcu , Akanıl Bingöl, Nüket . "In Vitro Propagation of Wetland Medicinal Plant Lythrum salicaria L.". Celal Bayar University Journal of Science 14 / 4 (December 2018): 369-372. https://doi.org/10.18466/cbayarfbe.393626