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Effect of whole body vibration on the serum dentin matrix protein level

Year 2014, , 116 - 119, 01.05.2014
https://doi.org/10.5505/abantmedj.2014.93064

Abstract

OBJECTIVE: Dentin matrix protein DMP is a mechanosensitive phosphoprotein expressed in osteocytes and associated with the extracellular matrix. The aim of this study was to determine whether serum DMP levels were affected by applying whole-body vibration WBV in healthy young adults.METHODS: Healthy eight volunteer men 20–45 years were recruited for the study. WBV was applied to the experimental group at frequency of 35 Hz, 2.2 mm peak-to-peak amplitude and duration of 45 seconds while standing the upright posture on the platform. Serum DMP levels was measured before and on the first, second, sixth, eighth, tenth, twelfth, fourteenth, sixteenth ve eightheenth minute after vibration. A researcher was blind to the measurement of samples. Serum DMP level were measured by Human Dentin Matrix Protein ELISA kit.RESULTS: Serum DMP level before WBV was 22.5 ± 5.8 ng / ml and there was no significant change after WBV F 2.2-13.3 = 1.45 P = 0.271 .CONCLUSION: In conclusion, the findings of this study reveals that serum DMP level in the healthy young adult men did not change in early period first 18 minutes after WBV .

References

  • Yang W, Lu Y, Kalajzic I, Guo D, Harris MA, Gluhak-Heinrich J, Kotha S, Bonewald LF, Feng JQ, Rowe DW, Turner CH, Robling AG, Harris SE. Dentin matrix protein 1 gene cis-regulation: use in osteocy- tes to characterize local responses to mechanical loading in vitro and in vivo. J Biol Chem 2005;280:680-90.
  • Kalajzic I, Braut A, Guo D, Jiang X, Kronenberg MS, Mina M, Harris MA, Harris SE, Rowe DW. Den- tin matrix protein 1 expression during osteoblastic differentiation, generation of an osteocyte GFPt- ransgene. Bone 2004;35:74-82.
  • Toyosawa S, Kanatani N, Shintani S, Kobata M, Yuki M, Kishino M, Ijuhin N, Komori T. Expression of dentin matrix protein 1 (DMP1) during fracture healing. Bone 2004;35:553-61.
  • Toyosawa S, Shintani S, Fujiwara T, Ooshima T, Sato A, Ijuhin N, Komori T.Dentin matrix protein 1 is predominantly expressed in chicken and rat oste- ocytes but not in osteoblasts. J Bone Miner Res 2001;16:2017-26.
  • Gluhak-Heinrich J, Ye L, Bonewald LF, Feng JQ, MacDougall M, Harris SE, Pavlin D. Mechanical loa- ding stimulates dentin matrix protein 1 (DMP1) expression in osteocytes in vivo. J Bone Miner Res 2003;18:807-17.
  • Wu H, Teng PN, Jayaraman T, Onishi S, Li J, Ban- non L, Huang H, Close J, Sfeir C. Dentin matrix pro- tein 1 (DMP1) signals via cell surface integrin. J Biol Chem 2011;286:29462-9.
  • Cochrane DJ. Vibration exercise: the potential benefits. Int J Sports Med 2011;32:75-99.
  • Delecluse C, Roelants M, Verschueren S. Strength increase after whole-body vibration compared with resistance training. Med Sci Sports Exerc 2003;35:1033-41.
  • Di Giminiani R, Manno R, Scrimaglio R, Sementilli S, Tihanyi J. Effects of individualized wholebody vibration on muscle flexibility and mechanical power. J Sports Med Phys Fitness 2010;50:139-51.
  • Sitjà-Rabert M, Rigau D, Fort Vanmeerghaeghe A, Romero-Rodríguez D, Bonastre Subirana M, Bon- fill X. Efficacy of whole body vibration exercise in older people:a systematic review. Disabil Rehabil 2012;34:883-93.
  • Wilcock IM, Whatman C, Harris N, Keogh JW. Vibration training: could it enhance the strength, power, or speed of athletes? J Strength Cond Res 2009;23:593-603.
  • Cidem M, Karakoc Y, Ekmekci H, Kucuk SH, Ulu- dag M, Gün K, Karamehmetoglu SS, Karacan I. Ef- fects of whole body vibration on plasma sclerostin level in healthy women. Turk J Med Sci DOI:10.3906/sag-1302-88
  • Karamehmetoglu SS, Karacan I, Cidem M, Kucuk SH, Ekmekci H, Bahadir C. Effects of osteocytes on vibration-induced reflex muscle activity in postme- nopausal women Turk J Med Sci DOI:10.3906/sag- 1305-3
  • Lombardi G, Lanteri P, Colombini A, Mariotti M, Banfi G. Sclerostin concentrations in athletes: role of load and gender. J Biol Regul Homeost Agents 2012;26:157-63.
  • Robling AG, Niziolek PJ, Baldridge LA, Condon KW, Allen MR, Alam I , Mantila SM, Gluhak-Heinrich J, Bellido TM, Harris SE, et al. Mechanical stimula- tion of bone in vivo reduces osteocyte expression of Sost/sclerostin. J Biol Chem 2008;283:5866-75.
  • Chen JH, Liu C, You L, Simmons CA. Boning up on Wolff's Law: mechanical regulation of the cells that make and maintain bone. J Biomech 2010;43:108- 18.
  • Sample SJ, Behan M, Smith L, Oldenhoff WE, Markel MD, Kalscheur VL, Hao Z, Miletic V, Muir P. Functional adaptation to loading of a single bone is neuronally regulated and involves multiple bones. J Bone Miner Res 2008;23:1372-81.

Dentin matriks protein serum düzeyi üzerine tüm vücut vibrasyonun etkisi

Year 2014, , 116 - 119, 01.05.2014
https://doi.org/10.5505/abantmedj.2014.93064

Abstract

AMAÇ: Dentin matriks protein DMP osteositlerde eksprese edilen ekstraselüler maktriks ile ilişkili mekanosensitif bir fosfoproteindir. Bu araştırmanın amacı, genç erişkin sağlıklı erkeklerde DMP serum düzeyinin tüm vücut vibrasyon TVV ile değişip değişmediğini ortaya koymaktır.YÖNTEMLER: Araştırmaya, 20-45 yaş arasında 8 sağlıklı gönüllü erkek denekler alındı. Deneklere TVV platformu üzerinde ayakta dik postürde dururken frekansı 35 Hz, tepe-tepe amplitüdü 2.2 mm, süresi 45 sn olan vibrasyon uygulandı. TVV öncesi bir kez ve vibrasyon seti sona erdikten sonra ikişer dakika ara ile 10 kez TVV’dan hemen sonra, 2.dk, 4.dk, 6.dk, 8.dk, 10.dk, 12.dk 14.dk, 16.dk ve 18.dk kan örneği alındı. Ölçümler bir araştırmacı tarafından, numunelere kör olarak yapıldı. Serum DMP düzeyi Human Dentin Matrix Protein ELISA kiti ile ölçüldü.BULGULAR: TVV öncesi serum DMP düzeyi 22.5 ± 5.8 ng/ml idi ve TVV uygulaması ile anlamlı değişim olmadığı saptandı F 2.2-13.3 = 1.45 P=0.271 .SONUÇ: Sonuç olarak, bu araştırma sağlıklı genç erişkin erkeklerde DMP serum düzeyinin erken dönemde TVV sonrası ilk 18 dakikada değişmediğini ortaya koymaktadır.

References

  • Yang W, Lu Y, Kalajzic I, Guo D, Harris MA, Gluhak-Heinrich J, Kotha S, Bonewald LF, Feng JQ, Rowe DW, Turner CH, Robling AG, Harris SE. Dentin matrix protein 1 gene cis-regulation: use in osteocy- tes to characterize local responses to mechanical loading in vitro and in vivo. J Biol Chem 2005;280:680-90.
  • Kalajzic I, Braut A, Guo D, Jiang X, Kronenberg MS, Mina M, Harris MA, Harris SE, Rowe DW. Den- tin matrix protein 1 expression during osteoblastic differentiation, generation of an osteocyte GFPt- ransgene. Bone 2004;35:74-82.
  • Toyosawa S, Kanatani N, Shintani S, Kobata M, Yuki M, Kishino M, Ijuhin N, Komori T. Expression of dentin matrix protein 1 (DMP1) during fracture healing. Bone 2004;35:553-61.
  • Toyosawa S, Shintani S, Fujiwara T, Ooshima T, Sato A, Ijuhin N, Komori T.Dentin matrix protein 1 is predominantly expressed in chicken and rat oste- ocytes but not in osteoblasts. J Bone Miner Res 2001;16:2017-26.
  • Gluhak-Heinrich J, Ye L, Bonewald LF, Feng JQ, MacDougall M, Harris SE, Pavlin D. Mechanical loa- ding stimulates dentin matrix protein 1 (DMP1) expression in osteocytes in vivo. J Bone Miner Res 2003;18:807-17.
  • Wu H, Teng PN, Jayaraman T, Onishi S, Li J, Ban- non L, Huang H, Close J, Sfeir C. Dentin matrix pro- tein 1 (DMP1) signals via cell surface integrin. J Biol Chem 2011;286:29462-9.
  • Cochrane DJ. Vibration exercise: the potential benefits. Int J Sports Med 2011;32:75-99.
  • Delecluse C, Roelants M, Verschueren S. Strength increase after whole-body vibration compared with resistance training. Med Sci Sports Exerc 2003;35:1033-41.
  • Di Giminiani R, Manno R, Scrimaglio R, Sementilli S, Tihanyi J. Effects of individualized wholebody vibration on muscle flexibility and mechanical power. J Sports Med Phys Fitness 2010;50:139-51.
  • Sitjà-Rabert M, Rigau D, Fort Vanmeerghaeghe A, Romero-Rodríguez D, Bonastre Subirana M, Bon- fill X. Efficacy of whole body vibration exercise in older people:a systematic review. Disabil Rehabil 2012;34:883-93.
  • Wilcock IM, Whatman C, Harris N, Keogh JW. Vibration training: could it enhance the strength, power, or speed of athletes? J Strength Cond Res 2009;23:593-603.
  • Cidem M, Karakoc Y, Ekmekci H, Kucuk SH, Ulu- dag M, Gün K, Karamehmetoglu SS, Karacan I. Ef- fects of whole body vibration on plasma sclerostin level in healthy women. Turk J Med Sci DOI:10.3906/sag-1302-88
  • Karamehmetoglu SS, Karacan I, Cidem M, Kucuk SH, Ekmekci H, Bahadir C. Effects of osteocytes on vibration-induced reflex muscle activity in postme- nopausal women Turk J Med Sci DOI:10.3906/sag- 1305-3
  • Lombardi G, Lanteri P, Colombini A, Mariotti M, Banfi G. Sclerostin concentrations in athletes: role of load and gender. J Biol Regul Homeost Agents 2012;26:157-63.
  • Robling AG, Niziolek PJ, Baldridge LA, Condon KW, Allen MR, Alam I , Mantila SM, Gluhak-Heinrich J, Bellido TM, Harris SE, et al. Mechanical stimula- tion of bone in vivo reduces osteocyte expression of Sost/sclerostin. J Biol Chem 2008;283:5866-75.
  • Chen JH, Liu C, You L, Simmons CA. Boning up on Wolff's Law: mechanical regulation of the cells that make and maintain bone. J Biomech 2010;43:108- 18.
  • Sample SJ, Behan M, Smith L, Oldenhoff WE, Markel MD, Kalscheur VL, Hao Z, Miletic V, Muir P. Functional adaptation to loading of a single bone is neuronally regulated and involves multiple bones. J Bone Miner Res 2008;23:1372-81.
There are 17 citations in total.

Details

Primary Language Turkish
Journal Section Research Article
Authors

Muharrem Çidem This is me

İlhan Karacan This is me

Mehmet Çidem This is me

Saadet Pilten Güzel This is me

Murat Özkaya This is me

Publication Date May 1, 2014
Published in Issue Year 2014

Cite

APA Çidem, M., Karacan, İ., Çidem, M., Güzel, S. P., et al. (2014). Dentin matriks protein serum düzeyi üzerine tüm vücut vibrasyonun etkisi. Abant Medical Journal, 3(2), 116-119. https://doi.org/10.5505/abantmedj.2014.93064
AMA Çidem M, Karacan İ, Çidem M, Güzel SP, Özkaya M. Dentin matriks protein serum düzeyi üzerine tüm vücut vibrasyonun etkisi. Abant Med J. May 2014;3(2):116-119. doi:10.5505/abantmedj.2014.93064
Chicago Çidem, Muharrem, İlhan Karacan, Mehmet Çidem, Saadet Pilten Güzel, and Murat Özkaya. “Dentin Matriks Protein Serum düzeyi üzerine tüm vücut Vibrasyonun Etkisi”. Abant Medical Journal 3, no. 2 (May 2014): 116-19. https://doi.org/10.5505/abantmedj.2014.93064.
EndNote Çidem M, Karacan İ, Çidem M, Güzel SP, Özkaya M (May 1, 2014) Dentin matriks protein serum düzeyi üzerine tüm vücut vibrasyonun etkisi. Abant Medical Journal 3 2 116–119.
IEEE M. Çidem, İ. Karacan, M. Çidem, S. P. Güzel, and M. Özkaya, “Dentin matriks protein serum düzeyi üzerine tüm vücut vibrasyonun etkisi”, Abant Med J, vol. 3, no. 2, pp. 116–119, 2014, doi: 10.5505/abantmedj.2014.93064.
ISNAD Çidem, Muharrem et al. “Dentin Matriks Protein Serum düzeyi üzerine tüm vücut Vibrasyonun Etkisi”. Abant Medical Journal 3/2 (May 2014), 116-119. https://doi.org/10.5505/abantmedj.2014.93064.
JAMA Çidem M, Karacan İ, Çidem M, Güzel SP, Özkaya M. Dentin matriks protein serum düzeyi üzerine tüm vücut vibrasyonun etkisi. Abant Med J. 2014;3:116–119.
MLA Çidem, Muharrem et al. “Dentin Matriks Protein Serum düzeyi üzerine tüm vücut Vibrasyonun Etkisi”. Abant Medical Journal, vol. 3, no. 2, 2014, pp. 116-9, doi:10.5505/abantmedj.2014.93064.
Vancouver Çidem M, Karacan İ, Çidem M, Güzel SP, Özkaya M. Dentin matriks protein serum düzeyi üzerine tüm vücut vibrasyonun etkisi. Abant Med J. 2014;3(2):116-9.