The effect of filgrastim on hemodynamic parameters in naive rats

Year 2025, Volume: 7 Issue: 6, 733 - 737, 26.10.2025

Hasan Çalışkan , Seda Koçak , Erhan Nalçacı

https://doi.org/10.38053/acmj.1756366

Abstract

Aims: Filgrastim, a recombinant form of granulocyte colony-stimulating factor (G-CSF), is used in neutropenic conditions. Apart from its use in neutropenia treatment, it is also employed in experimental studies due to its neuroprotective effects. This study aimed to investigate the effect of filgrastim on hemodynamic parameters in rats.
Methods: 24 adult male Wistar albino rats were used. The rats were divided into three groups: control, 50 μg filgrastim group, and 100 μg filgrastim group (n=8). Filgrastim was administered intraperitoneally once daily in a dose-dependent manner for a total of 5 days. The control group received 1 ml/kg of saline, following a similar treatment protocol. After 5 days of filgrastim administration, blood pressure measurements were taken in all groups of rats using indirect tail-cuff plethysmography. A total of five measurements were taken at 1-minute intervals for each animal.
Results: Systolic pressure (control: 117.40±7.93 mmHg, 50μg: 118.50±17.25 mmHg, 100 μg: 127.70±14.94 mmHg), diastolic pressure (control: 92.42±8.76 mmHg 50 ug: 95.65±8.75 mmHg, 100 μg: 101.20±8.04 mmHg) and mean arterial pressure (control: 100.70±7.61 mmHg 50μg: 103.20±10.62 mmHg 100 μg: 110.00±9.91 mmHg) were not significantly different among experiment groups (p>0.05). Heart rates showed a remarkable increase in the group administered 100 μg (366.5±22.35) compared to the control group, while no significant increase was observed in the group administered 50 μg (348±15.69).
Conclusion: In our study, no consistent changes in systolic blood pressure were observed across treatment groups. However, a modest increase in heart rate was observed at the higher dose, suggesting a potential dose-related cardiovascular effect after 5 days of Filgrastim administration. Long-term effects of similar and higher doses on hemodynamic parameters can be examined.

Keywords

Blood pressure , filgrastim , heart rate , neutropenia

Ethical Statement

All experiments were conducted in accordance with the guidelines approved by the Ankara University Experimental Animals Ethics Committee, with the approval reference number 2023-9-76 (committee meeting date: May 10, 2023).

Supporting Institution

Expenditures, including drugs and chemicals, did not receive specific grants from public, commercial, or not-for-profit funding agencies. Only animal prices were provided by the Council of Higher Education Faculty Member Training Program.

Thanks

We want to thank to Vet. Atilla ISGOREN and Bio. Nazli AYDIN for their help in providing animals.

Filgrastimin sağlıklı sıçanlarda hemodinamik parametreler üzerindeki etkisi

Abstract

Amaç: Granülosit koloni uyarıcı faktörün (G-CSF) rekombinant bir formu olan filgrastim, nötropenik durumlarda kullanılır. Nötropeni tedavisinde kullanımının yanı sıra, nöroprotektif etkileri nedeniyle deneysel çalışmalarda da kullanılmaktadır. Bu çalışmada, filgrastimin sıçanlarda hemodinamik parametreler üzerindeki etkisi araştırılmıştır. Yöntemler: 24 yetişkin erkek Wistar albino sıçan kullanılmıştır. Sıçanlar kontrol, 50 μg filgrastim grubu ve 100 μg filgrastim grubu olmak üzere üç gruba ayrılmıştır (n=8). Filgrastim, doz bağımlı bir şekilde günde bir kez intraperitoneal olarak toplam 5 gün boyunca uygulanmıştır. Kontrol grubuna benzer bir tedavi protokolü izlenerek 1 mL/kg salin verildi. Filgrastim uygulamasının 5. gününde, tüm sıçan gruplarında dolaylı kuyruk manşet pletismografisi kullanılarak kan basıncı ölçümleri yapıldı. Her hayvan için 1 dakikalık aralıklarla toplam beş ölçüm yapıldı.

Sonuç: Sistolik basınç (Kontrol: 117,4±7,93, 50 ug: 118,5±17,25 100 ug: 127,7±14,94), diyastolik basınç (Kontrol: 92,42±8,76 50 ug: 95,65±8,75 100 ug: 101,2±8,04) ve ortalama arter basıncı (Kontrol: 100,7±7,61 50 ug: 103,2±10,62 100 ug: 110,0±9,91) deney grupları arasında önemli bir fark göstermedi (p>0,05). Kalp atış hızları, kontrol grubuna kıyasla 100 μg uygulanan grupta (366,5±22,35) belirgin bir artış gösterirken, 50 μg uygulanan grupta (348±15,69) önemli bir artış gözlenmemiştir.

Sonuç: Filgrastim'in 5 günlük uygulamasından sonra kısa vadeli hemodinamik parametrelerde önemli bir değişiklik gözlenmemiştir. Benzer ve daha yüksek dozların hemodinamik parametreler üzerindeki uzun vadeli etkileri incelenebilir.

Keywords

kan basıncı , filgrastim , kalp atım hızı , nötropeni

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