Sıçanlarda östrüs siklusuda endometriyum dokusunda humoral savunma hücrelerinin dağılımı

Year 2016, , 208 - 2019, 15.04.2016

Alper Yalçın , Mehmet Kanter

https://doi.org/10.30569/adiyamansaglik.418086

Abstract

Amaç: Bu çalışmada, östrüs siklusu
(proöstrüs, östrüs, metöstrüs, diöstrüs) boyunca endometriyumda görülen humoral savunma sistemi
hücreleri dağılımının histokimyasal olarak belirlenmesi amaçlanmıştır.

Yöntem: Ağırlıkları 200-250g arasında değişen üç aylık 28 adet
Wistar Albino dişi sıçan
(kullanıldı) incelemeye alındı. Sıçanlar, östrüs siklus evrelerine göre her
grupta 7 adet sıçan olacak şekilde 4 gruba ayrıldı. Östrüsun dönemlerini belirlemek
için, vajinal smear yöntemi uygulandı.
Alınan sürüntü temiz bir lam üzerine
yayılarak metilen
mavisi ile boyandı. Bu bloklardan alınan 6 µ kalınlığındaki kesitler plazma hücrelerini belirlemek için metil green-pironin boyama
yöntemi uygulandı.

Bulgular: Plazma hücrelerinin en fazla sayıda proöstrüs grubundaki sıçanlarda
görüldü. Plazma hücre sayısının sırasıyla östrüs, metöstrus ve diöstrus
evrelerine doğru oldukça azaldığı belirlendi.

Sonuç: Endometriyumdaki
humoral savunma sistemi hücrelerinin, östrüs siklusunun farklı evrelerinde
değişik dağılımlar gösterdiği sonucuna varıldı. Östrüs siklusunun, hormonal
regülasyonun kontrolü altında birbirini takip eden farklı evreler içerdiği ve
evreler arasındaki immunolojik tepkilerdeki farklılığın yine bu hormonal
kontroltü altında ortaya çıktığı kanaatine varıldı.

Keywords

humoral savunma sistemi, östrüs siklusu, endometriyum

Distribution of humoral defense cells in the endometrium during the estrus cycle in rats

Abstract

Purpose:
In this study, it was histochemically
aimed to determine the distribution of humoral defense system cells in the
endometrium during oestrous cycle (proöstrüs, östrüs, metöstrüs, diöstrüs).

Method:
In this study, 28 Wistar Albino
female rats (used), weighing 200-250 g and 3 months old, were examined. The
rats were divided into 4 groups according to the estrous cycle stages as 7 rats
at each group. Vaginal smear method was used to determine estrous periods. The
receiving material was spread on a clean lam and painted with methylene blue.
Methyl green-pyronin staining method was used to identify plasma cells of 6 μ
thickness sections from parafine blocks.

Results:
The largest number of plasma cells
were seen in the proestrous group.

Plasma cell numbers were
significantly reduced towards oestrous, metrostrus and diestrus stages,
respectively.

Conclusion:
Humoral defense system cells in the
endometrium were found to show different distributions at different stages of
the oestrous cycle. The oestrous cycle contains different  stages under the control of hormonal
regulation. It was concluded that the differences in the immunological
responses between these stages also appeared under this hormonal control.

Keywords

humoral defense system, estrous cycle, endometrium

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