Comparison of Antioxidant mRNA expression levels of advanced Protruded and Extruded Nucleus Pulposus in Degenerative Lumbar Disc Herniations using the RT-PCR method

Year 2019, , 1157 - 1167, 15.04.2019

Şeyho Cem Yücetaş , Necati Üçler

https://doi.org/10.30569/adiyamansaglik.486946

Abstract

Purpose: The aim of the present study is to compare the antioxidant levels of
nucleus pulposus in advanced protruded and extruded lumbar disc hernias (LDH)
with a steady lumbar posterior longitudinal ligament with the Real
Time-Polymerase Chain Reaction (RT-PCR) method.

Method: This
study was conducted with the comparison of the disc sections of a total of 40
patients who underwent an operation due to advanced protruded (n=20) and extruded
(n=20) LDH with the TRI-Reagent^® and Ribonucleic acid (RNA)
isolation and the RT-PCR method between January 2013 and May 2016. The study
included patients diagnosed with lumbar disc herniation who were applied with microdiscectomy
surgery. The antioxidant levels of the disc material, which caused compression
because it was highly protrudedorextruded, were detected using the RT-PCR
method,and the expression levels of the genes were determined.

Results: The antioxidant levels of the disc materials of a total of 40 patients, who
underwent an operation due to protruded and extruded disc hernia, were
determined with the RT-PCR method. The patients were found 25 females with a
mean age of 36.4 years and 15 males with a mean age of 39.26 years. Of the total
patients, 20 had MacNab grade 2 (protruded) and 20 had  MacNab grade 3 (extruded) disc hernias. The
expressions of protruded LDH materials were found to be lower than those of extruded
LDH materials.

Conclusion: Although the
expression levels of the significant antioxidant molecules of TGF-β, FGF-β,
IGF-1, NGF, MMP-3, and GAPDH mRNA, in patients with extruded LDH were
significantly higher compared to those with protruded LDH, no distinctive
features of these levels could be determined in terms of discogenic pain and
postoperative clinical improvement.

Keywords

Lumbar microdiscectomy, extruded disc, RT-PCR, Gene Expression

RT-PCR yöntemi kullanılarak Dejeneratif Lomber Disk Herniasyonunda İleri Düzey Protrüde ve Ekstrüde Nükleus Pulposusun Antioksidan mRNA ekspresyon düzeylerinin Karşılaştırılması

Abstract

Amaç. Bu
çalışmanın amacı, Real Time-Polimeraz Zincir Reaksiyonu (RT-PCR) yöntemi ile lumbar
posterior longitudinal ligamentte ileri düzey protrude ve ekstrüde lomber disk
hernilerinde (LDH) nükleus pulposusun antioksidan düzeylerini karşılaştırmaktır.

Metot: Bu
çalışma, Ocak 2013 ile Mayıs 2016 arasında izolasyon ve RT-PCR yöntemi ile ileri
düzey protrüde (n = 20) ve ekstrüde (n = 20) LDH ile TRI-Reagent® ve
Ribonükleik asit (RNA) ile ameliyat edilen toplam 40 hastanın disk bölümlerinin
karşılaştırılması ile yapıldı. Çalışmaya, mikrodisektomi cerrahisi uygulanan
lomber disk herniasyonu tanısı alan hastalar dahil edildi. Sıkışmaya neden olan
disk materyalinin antioksidan seviyeleri, RT-PCR yöntemi kullanılarak tespit
edildi ve genlerin ekspresyon seviyeleri belirlendi.

Bulgular: Protrude ve ekstrüde disk hernisi nedeniyle ameliyat edilen toplam 40
hastanın disk materyallerinin antioksidan düzeyleri RT-PCR yöntemi ile
belirlendi. Çalışmadaki 25 kadın hastanın yaş ortalaması 36.4 ve 15 erkek hastanın
yaş ortalaması 39.26 olduğu belirlendi. Toplamda hastaların 20'sinde MacNab
grade 2 (protruded) ve 20'sinde MacNab grade 3 (ekstrüde) disk fıtığı vardı. Ekspresyon
sonucunda  protrüde LDH materyallerinin
ekstrüde LDH materyallerinden daha düşük olduğu bulundu.

Sonuç: Ekstrüde
LDH'si olan hastalarda TGF-β, FGF-β, IGF-1, NGF, MMP-3 ve GAPDH mRNA'nın
anlamlı antioksidan moleküllerinin ekspresyon seviyeleri, protrüde LDH hastalara
göre anlamlı olarak daha yüksek bulunmasına rağmen, bu düzeylerin diskojenik
ağrı ve postoperative  klinik iyileşme
açısından ayırt edici özellik olarak belirlenemedi.

Keywords

Lomber mikrodiskektomi, ekstrüde disk, RT-PCR, Gen İfadesi

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