Evaluation of The Cytotoxicity of Different Composite Materials by Two Different Cell Culture Test

Yıl 2008, Cilt: 2 Sayı: 2, 98 - 103, 01.03.2008

Bilge Turhan Bal Nalan Ayhan Sönmez Barkın Bavbek Cihan Akçaboy

Öz

Toxicity of dental materials has caused public concern over the past years. The aim of this study was to evaluate the cytotoxicity of the 3 different resin composites with 2 different cell culture test models. MTT and cell attachment assay were used in the present study. Results were evaluated by two-way analysis of variance and Duncan’s test and in each group, no significant difference was found between the materials and control on the 2nd day whereas on the 5th and 7th day, it was found that the composite resin materials showed cytotoxic effect and the differences between the materials were statistically significant. In this study it was determined that the results of each cell culture test MTT and Cell attachment test were different

Anahtar Kelimeler

Resin composites, cytotoxicity, cell culture tests

Farklı Kompozit Materyallerinin Sitotoksisitelerinin İki Farklı Hücre Kültürü Test Yöntemiyle Değerlendirilmesi

Öz

Dental materyallerin toksisitesi geçmiş yıllardan beri genel bir endişeye neden olmuştur. Bu çalışmanın amacı 3 farklı kompozit materyalinin sitotoksisitelerinin, 2 farklı hücre kültürü test yöntemiyle değerlendirilmesidir. Çalışmada MTT ve hücre tutunma testleri kullanılmıştır. Sonuçlar, iki yönlü varyans analizi tekniği ve Duncan testiyle değerlendirilmiş ve her iki grupta 2. günde materyaller ve kontrol grubu arasında önemli bir farklılık gözlenmezken, 5. ve 7. günde kompozit materyallerinin sitotoksik etki gösterdiği ve materyaller arasındaki farklılıkların istatistik olarak önemli olduğu bulunmuştur. Çalışmada MTT ve hücre tutunma testinden elde edilen sonuçların da farklılık gösterdiği saptanmıştır

Anahtar Kelimeler

kompozit rezinler, sitotoksisite, hücre kültürü testleri

Kaynakça

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