Research Article

Effects of Different Doses of Royal Jelly on Oxidative Stress Parameters in Streptozotocin-Induced Diabetic Rats

Volume: 15 Number: 4 October 27, 2026
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Effects of Different Doses of Royal Jelly on Oxidative Stress Parameters in Streptozotocin-Induced Diabetic Rats

Abstract

Chronic hyperglycemia in diabetes increases the cellular oxidant burden while weakening antioxidant defense capacity. Royal jelly has been suggested to exert antioxidant effects; however, its tissue-specific activity at different doses remains unclear. The present study investigated malondialdehyde (MDA) concentrations and superoxide dismutase (SOD) activities in serum, brain, kidney, heart, and testis samples obtained from male rats with streptozotocin (STZ)-induced diabetes after royal jelly treatment. Thirty-six adult male Sprague–Dawley rats were allocated to healthy control, diabetic control, healthy royal jelly (300 mg/kg), and diabetic royal jelly groups receiving 50, 150, or 300 mg/kg. A 50 mg/kg dose of STZ was administered intraperitoneally to establish diabetes, and royal jelly was given orally once daily for four weeks. Biochemical measurements were performed spectrophotometrically. Serum MDA concentrations were comparable among groups, whereas serum SOD activity showed a significant group effect (p = 0.005), with lower activity in diabetic controls than in the healthy control and healthy royal jelly groups. Renal MDA was elevated and renal SOD activity was reduced in diabetic controls relative to the healthy groups (p < 0.001 and p = 0.007, respectively). Cardiac MDA remained unchanged, while cardiac SOD activity was lower in diabetic controls than in healthy controls (p = 0.008). No significant group differences were detected in brain or testicular parameters (p > 0.05). Royal jelly did not significantly reduce renal MDA; nevertheless, SOD activities in serum, kidney, and heart tended to be higher in treated diabetic groups than in diabetic controls and were comparable to healthy control values. These results indicate that royal jelly did not clearly suppress lipid peroxidation but may modestly limit the diabetes-related decline in antioxidant defense.

Keywords

Supporting Institution

No specific funding was received for this study.

Ethical Statement

This study approved by the Aydın Adnan Menderes University Animal Experiments Local Ethics Committee (ADÜ-HADYEK; decision dated 24 November 2021, no. 64583101/2021/159).

Thanks

No specific funding was received for this study.

References

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Details

Primary Language

English

Subjects

Veterinary Sciences (Other)

Journal Section

Research Article

Publication Date

October 27, 2026

Submission Date

August 6, 2026

Acceptance Date

September 29, 2026

Published in Issue

Year 2026 Volume: 15 Number: 4

APA
Şahiner, H. S., Ak, M. O., Bardakçı Yılmaz, Ö., Avcıoğlu, M., Gündeş Kut, S., Sekkin, S., Boyacıoğlu, M., & Kum, C. (2026). Effects of Different Doses of Royal Jelly on Oxidative Stress Parameters in Streptozotocin-Induced Diabetic Rats. Animal Health Production and Hygiene, 15(4). https://doi.org/10.53913/aduveterinary.2012739
AMA
1.Şahiner HS, Ak MO, Bardakçı Yılmaz Ö, et al. Effects of Different Doses of Royal Jelly on Oxidative Stress Parameters in Streptozotocin-Induced Diabetic Rats. Animal Health, Prod and Hyg. 2026;15(4). doi:10.53913/aduveterinary.2012739
Chicago
Şahiner, Hande Sultan, Mehmet Onur Ak, Özge Bardakçı Yılmaz, et al. 2026. “Effects of Different Doses of Royal Jelly on Oxidative Stress Parameters in Streptozotocin-Induced Diabetic Rats”. Animal Health Production and Hygiene 15 (4). https://doi.org/10.53913/aduveterinary.2012739.
EndNote
Şahiner HS, Ak MO, Bardakçı Yılmaz Ö, Avcıoğlu M, Gündeş Kut S, Sekkin S, Boyacıoğlu M, Kum C (October 1, 2026) Effects of Different Doses of Royal Jelly on Oxidative Stress Parameters in Streptozotocin-Induced Diabetic Rats. Animal Health Production and Hygiene 15 4
IEEE
[1]H. S. Şahiner et al., “Effects of Different Doses of Royal Jelly on Oxidative Stress Parameters in Streptozotocin-Induced Diabetic Rats”, Animal Health, Prod and Hyg, vol. 15, no. 4, Oct. 2026, doi: 10.53913/aduveterinary.2012739.
ISNAD
Şahiner, Hande Sultan - Ak, Mehmet Onur - Bardakçı Yılmaz, Özge - Avcıoğlu, Merve - Gündeş Kut, Sultan - Sekkin, Selim - Boyacıoğlu, Murat - Kum, Cavit. “Effects of Different Doses of Royal Jelly on Oxidative Stress Parameters in Streptozotocin-Induced Diabetic Rats”. Animal Health Production and Hygiene 15/4 (October 1, 2026). https://doi.org/10.53913/aduveterinary.2012739.
JAMA
1.Şahiner HS, Ak MO, Bardakçı Yılmaz Ö, Avcıoğlu M, Gündeş Kut S, Sekkin S, Boyacıoğlu M, Kum C. Effects of Different Doses of Royal Jelly on Oxidative Stress Parameters in Streptozotocin-Induced Diabetic Rats. Animal Health, Prod and Hyg. 2026;15. doi:10.53913/aduveterinary.2012739.
MLA
Şahiner, Hande Sultan, et al. “Effects of Different Doses of Royal Jelly on Oxidative Stress Parameters in Streptozotocin-Induced Diabetic Rats”. Animal Health Production and Hygiene, vol. 15, no. 4, Oct. 2026, doi:10.53913/aduveterinary.2012739.
Vancouver
1.Hande Sultan Şahiner, Mehmet Onur Ak, Özge Bardakçı Yılmaz, Merve Avcıoğlu, Sultan Gündeş Kut, Selim Sekkin, Murat Boyacıoğlu, Cavit Kum. Effects of Different Doses of Royal Jelly on Oxidative Stress Parameters in Streptozotocin-Induced Diabetic Rats. Animal Health, Prod and Hyg. 2026 Oct. 1;15(4). doi:10.53913/aduveterinary.2012739