Effects of Different Doses of Royal Jelly on Oxidative Stress Parameters in Streptozotocin-Induced Diabetic Rats

Öz

Chronic hyperglycemia in diabetes increases the cellular oxidant burden while weakening antioxidant defense capacity. Royal jelly has been suggested to exert antioxidant effects; however, its tissue-specific activity at different doses remains unclear. The present study investigated malondialdehyde (MDA) concentrations and superoxide dismutase (SOD) activities in serum, brain, kidney, heart, and testis samples obtained from male rats with streptozotocin (STZ)-induced diabetes after royal jelly treatment. Thirty-six adult male Sprague–Dawley rats were allocated to healthy control, diabetic control, healthy royal jelly (300 mg/kg), and diabetic royal jelly groups receiving 50, 150, or 300 mg/kg. A 50 mg/kg dose of STZ was administered intraperitoneally to establish diabetes, and royal jelly was given orally once daily for four weeks. Biochemical measurements were performed spectrophotometrically. Serum MDA concentrations were comparable among groups, whereas serum SOD activity showed a significant group effect (p = 0.005), with lower activity in diabetic controls than in the healthy control and healthy royal jelly groups. Renal MDA was elevated and renal SOD activity was reduced in diabetic controls relative to the healthy groups (p < 0.001 and p = 0.007, respectively). Cardiac MDA remained unchanged, while cardiac SOD activity was lower in diabetic controls than in healthy controls (p = 0.008). No significant group differences were detected in brain or testicular parameters (p > 0.05). Royal jelly did not significantly reduce renal MDA; nevertheless, SOD activities in serum, kidney, and heart tended to be higher in treated diabetic groups than in diabetic controls and were comparable to healthy control values. These results indicate that royal jelly did not clearly suppress lipid peroxidation but may modestly limit the diabetes-related decline in antioxidant defense.

Anahtar Kelimeler

• Diabetes
• malondialdehyde
• oxidative stress
• royal jelly
• superoxide dismutase

Destekleyen Kurum

No specific funding was received for this study.

Etik Beyan

This study approved by the Aydın Adnan Menderes University Animal Experiments Local Ethics Committee (ADÜ-HADYEK; decision dated 24 November 2021, no. 64583101/2021/159).

Teşekkür

No specific funding was received for this study.

Streptozotosin ile Diyabet Oluşturulan Sıçanlarda Farklı Dozlarda Arı Sütünün Oksidatif Stres Parametreleri Üzerine Etkileri

Öz

Diyabette kronik hiperglisemi, hücresel oksidan yükü artırırken antioksidan savunma kapasitesini zayıflatır. Arı sütünün antioksidan etkilere sahip olabileceği bildirilmiş olmakla birlikte, farklı dozlarda dokuya özgü etkileri yeterince açıklığa kavuşmamıştır. Bu çalışmada, streptozotosin (STZ) ile diyabet oluşturulan erkek sıçanlarda arı sütü uygulaması sonrasında serum, beyin, böbrek, kalp ve testis örneklerindeki malondialdehit (MDA) düzeyleri ile süperoksit dismutaz (SOD) aktiviteleri araştırıldı. Otuz altı erişkin erkek Sprague–Dawley sıçan; sağlıklı kontrol, diyabetik kontrol, sağlıklı arı sütü (300 mg/kg) ve 50, 150 veya 300 mg/kg arı sütü uygulanan diyabetik gruplara ayrıldı. Diyabet oluşturmak amacıyla STZ, 50 mg/kg dozunda intraperitoneal olarak uygulandı ve arı sütü dört hafta boyunca günde bir kez oral yolla verildi. Biyokimyasal ölçümler spektrofotometrik olarak yapıldı. Serum MDA düzeyleri gruplar arasında benzerken, serum SOD aktivitesinde anlamlı grup etkisi saptandı (p = 0,005); diyabetik kontrol grubundaki aktivite sağlıklı kontrol ve sağlıklı arı sütü gruplarından daha düşüktü. Diyabetik kontrolde böbrek MDA düzeyi yükselmiş, böbrek SOD aktivitesi azalmıştı (sırasıyla p < 0,001 ve p = 0,007). Kalp MDA düzeyleri değişmezken, kalp SOD aktivitesi diyabetik kontrolde sağlıklı kontrolden daha düşüktü (p = 0,008). Beyin ve testis parametrelerinde anlamlı fark bulunmadı (p > 0,05). Arı sütü böbrek MDA düzeyini anlamlı olarak azaltmadı; ancak serum, böbrek ve kalp SOD aktiviteleri tedavi edilen diyabetik gruplarda diyabetik kontrolden sayısal olarak daha yüksek ve sağlıklı kontrol değerlerine yakındı. Bulgular, arı sütünün lipid peroksidasyonunu belirgin biçimde baskılamadığını, ancak diyabete bağlı antioksidan savunma kaybını sınırlı ölçüde azaltabileceğini göstermektedir.

Anahtar Kelimeler

• Arı sütü
• diyabet
• malondialdehit
• oksidatif stres
• süperoksit dismutaz

Destekleyen Kurum

Bu çalışma için herhangi bir özel finansman desteği alınmamıştır.

Etik Beyan

Bu çalışma Aydın Adnan Menderes Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu (ADÜ-HADYEK; 24 Kasım 2021 tarihli, 64583101/2021/159 numaralı karar) tarafından onaylanmıştır.

Teşekkür

Bu çalışma için herhangi bir özel finansman desteği alınmamıştır.

Kaynakça

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