Bioactive Secondary Metabolite Profile of Streptomyces netropsis DT02 and Its Biocontrol Potential against Some Phytopathogenic Fungi

Year 2026, , 1 - 10

Çiğdem Yüksel Demirci , Mustafa Oskay

https://doi.org/10.17097/agricultureatauni.1741687

Abstract

Actinobacteria represent a significant portion of soil microbial biomass and are renowned for their capacity to produce a wide variety of antibiotics and extracellular enzymes. Recently, researchers have concentrated on Actinobacteria with bioactive secondary metabolites in agriculture as biocontrol agents against phytopathogenic fungi. This study explores the isolation, identification, and metabolic profile of Streptomyces netropsis DT02, and evaluates its antagonistic potential against several phytopathogenic fungi, notably blue mold disease caused by Penicillium italicum in post-harvest citrus fruits. The extract exhibited vigorous antifungal activity in vitro, with inhibition zones ranging from 15 to 45 mm in diameter. The chemical metabolite profile of the crude extract of DT02 was detected using high-performance liquid chromatography-high-resolution electrospray ionization mass spectrometry, and the antifungal compounds were identified based on existing literature. The results showed that DT02 produced 13 different metabolites, including pimprinine, amphotericin B, pimprinethine, N-acetylaureothamine, prothracarcin, and aureothin. The results of these analyses indicated that the antagonistic activity of DT02 is quite substantial. This research creates opportunities for utilising metabolites from DT02 in the biocontrol of some phytopathogenic fungi in crops.

Keywords

Biocontrol , LC-HRESIMS , Penicillium italicum , Post-harvest disease , Secondary metabolites , Streptomyces netropsis

Ethical Statement

There are no ethical issues related to the research

Supporting Institution

Manisa Celal Bayar University Scientific Research Projects Coordination Unit

Project Number

2024-120

Thanks

This research was partially supported by the Manisa Celal Bayar University Scientific Research Projects Coordination Unit under project number 2024-120.

Streptomyces netropsis DT02'nin Biyoaktif Sekonder Metabolit Profili ve Bazı Fitopatojen Küflere Karşı Biyokontrol Potansiyeli

Abstract

Aktinobakteriler, toprak mikrobiyal biyokütlesinin önemli bir bölümünü oluşturur ve çok çeşitli antibiyotikler ve hücre dışı enzimler üretme kapasiteleriyle bilinirler. Son zamanlarda araştırmacılar, tarımda fitopatojen küflere karşı biyokontrol ajanları olarak biyoaktif sekonder metabolitlere sahip Aktinobakterilere odaklanmıştır. Bu çalışma, Streptomyces netropsis DT02'nin izolasyonunu, identifikasyonunu ve metabolik profilini incelemekte ve hasat sonrası turunçgillerde Penicillium italicum'un neden olduğu mavi küf hastalığı başta olmak üzere çeşitli fitopatojen küflere karşı antagonistik potansiyelini araştırmaktadır. Ekstre, in vitro ortamda güçlü antifungal aktivite göstermiş ve inhibisyon zonları 15 ila 45 mm arasında değişmiştir. DT02'nin ham ekstresinin kimyasal metabolit profili, yüksek performanslı sıvı kromatografisi-yüksek çözünürlüklü elektrosprey iyonizasyon kütle spektrometrisi kullanılarak tespit edilmiş ve antifungal bileşikler mevcut literatüre dayanarak tanımlanmıştır. Sonuçlar, DT02'nin pimprinin, amfoterisin B, pimprinetin, N-asetilüretamin, protrakarsin ve aureotin dahil olmak üzere 13 farklı metabolit ürettiğini göstermiştir. Bu analizlerin sonuçları, DT02'nin antagonistik aktivitesinin oldukça önemli olduğunu ortaya çıkarmıştır. Bu araştırma, DT02'den elde edilen metabolitlerin, mahsullerde bazı fitopatojenik küflerin biyolojik kontrolünde kullanılması için fırsatlar yaratmaktadır.

Keywords

Biyolojik kontrol , LC-HRESIMS , Penicillium italicum , Hasat sonrası hastalık , Sekonder metabolitler , Streptomyces netropsis

Project Number

2024-120

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