Ananas kabuğu ekstraktlarının in vitro antibakteriyal aktivitesi ve dimetil sülfoksit ekstraktının kimyasal kompozisyonu

Abstract

Bu çalışmada, ananas (Ananas comosus L.) kabuğundan ultrasonikasyonla elde edilen etanol, metanol ve dimetilsülfoksit (DMSO) ekstraktlarının antibakteriyel aktivitesi in vitro agar kuyu difüzyon ve mikrodilüsyon yöntemleriyle değerlendirildi. Agar kuyu difüzyon yöntemiyle elde edilen ananas kabuğu ekstraktlarının test edilen bakterilere (Escherichia coli (ATCC 1301, ATCC 25922, ATCC 8739 ve NRRL B-3704), Klebsiella pneumoniae ATCC 700603, Salmonella enterica subsp. enterica ATCC 13076, Salmonella enterica subsp. typhimurium ATCC 51812, Pseudomonas aeruginosa (ATCC 10145 ve ATCC 27853), Bacillus cereus, Bacillus subtilis, Enterococcus faecalis ATCC 29212 ve Staphylococcus aureus ATCC 29213 karşı herhangi bir inhibisyon bölgesi oluşturmadığı belirlendi. Ayrıca, mikrodilüsyon yöntemi kullanılarak MİK değerlerinin >125 µL/mL olduğu tespit edildi. Bununla birlikte, ananas kabuğundan elde edilen DMSO ekstraktının kimyasal kompozisyonu headspace katı faz mikroekstraksiyon–gaz kromatografisi–kütle spektrometresi (HS-SPME-GC-MS) yöntemi ile belirlendi ve sonuç olarak ana bileşenin flavanon yapısındaki pinostrobin (%44,66) olduğu belirlendi. Sonuç olarak, ananas kabuğundan elde edilen ekstraktların antibakteryel aktiviteye sahip olmadığı ve kimyasal kompozisyonu ile elde edilen sonuçların tutarlı olduğu belirlendi. Bu çalışma, bitki ve gıda atıklarının biyolojik potansiyellerinin belirlenmesinde kimyasal kompozisyon ile in vitro biyolojik aktivitelerin birlikte değerlendirilmesinin önemini göstermektedir.

Keywords

• Ananas kabuğu
• ekstrakt
• atıkların değerlendirilmesi
• kimyasal kompozisyon
• in vitro antibakteriyel aktivite

Supporting Institution

Bu çalışma, TÜBİTAK 2209-A programı kapsamında 1919B012477207 proje numarasıyla desteklenmiştir.

Project Number

1919B012477207

In vitro antibacterial activity of pineapple peel extracts and chemical composition of dimethyl sulfoxide extract

Abstract

In this study, ethanol, methanol, and dimethyl sulfoxide (DMSO) extracts were obtained from pineapple (Ananas comosus L.) peel by ultrasonication, and their antibacterial activity was evaluated in vitro using the agar well diffusion and microdilution methods. Pineapple peel extracts obtained by the agar well diffusion method did not form any inhibitory zones against the tested bacteria (Escherichia coli (ATCC 1301, ATCC 25922, ATCC 8739, and NRRL B-3704), Klebsiella pneumoniae ATCC 700603, Salmonella enterica subsp. enterica ATCC 13076, and Salmonella enterica subsp. typhimurium ATCC 51812, Pseudomonas aeruginosa (ATCC 10145 and ATCC 27853), Bacillus cereus, Bacillus subtilis, Enterococcus faecalis ATCC 29212, and Staphylococcus aureus ATCC 29213). Furthermore, MIC values were determined to be >125 µL/mL by microdilution. Moreover, the chemical composition of the DMSO extract from pineapple peel was determined by headspace solid-phase microextraction–gas chromatography–mass spectrometry (HS-SPME-GC-MS), and the main component was identified as pinostrobin (44.66%), a flavanone. In conclusion, the extracts obtained from pineapple peel do not exhibit antibacterial activity, and the results are consistent with the chemical composition. This study demonstrates the importance of evaluating chemical composition together with in vitro biological activities in determining the biological potential of plant and food wastes

Keywords

• Pineapple peel
• extract
• waste utilization
• chemical composition
• in vitro antibacterial activity

Supporting Institution

This study was supported by the TÜBİTAK 2209-A program under project number 1919B012477207.

Project Number

1919B012477207

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