Antimicrobial activity of green-synthesized MgO nanoparticles by using Sideritis perfoliata and Thymbra spicata var. spicata extracts

Abstract

In the present study, MgO nanoparticles (NPs) were green-synthesized using Sideritis perfoliata (SPE) and Thymbra spicata var. spicata (TSE) extracts. XRD and SEM analyses were used for the characterization of these NPs. Furthermore, green-synthesized MgO NPs were evaluated against various strains (E. faecalis, Y. enterocolitica, E. coli, S. aureus, P. aeruginosa, A. baumannii) to determine antibacterial activity. XRD data reveal the polycrystalline cubic structure of the green-synthesized MgO NPs. SEM images of MgO NPs produced with SPE and TSE showed that they had smooth surfaces and approximately spherical and crescent-like forms, respectively. MgO nanoparticles produced using SPE and TSE extracts were found to have noticeably different sizes, approximately 80 and 250 nm, respectively. Additionally, the antibacterial responses of the tested strains varied depending on the plant extract used, with the largest inhibition zones observed against A. baumannii for TSE-derived MgO NPs (13.0 ± 1.0 mm) and E. faecalis for SPE-derived MgO NPs (9.0 ± 0.5 mm). The present study is the first report on the antibacterial activity of green-synthesized MgO NPs by using extracts of S. perfoliata and T. spicata var. spicata collected from Osmaniye province (The East Mediterranean Region) of Türkiye

Keywords

• Antibacterial
• green synthesis
• nanoparticle
• Sideritis
• Thymbra

Sideritis perfoliata ve Thymbra spicata var. spicata ekstraktları kullanılarak yeşil sentez yöntemiyle üretilen MgO nanopartiküllerinin antimikrobiyal aktiviteleri

Abstract

Bu çalışmada, Sideritis perfoliata (SPE) ve Thymbra spicata var. spicata (TSE) ekstraktları kullanılarak MgO nanopartikülleri (NP’ler) yeşil sentez yöntemiyle üretilmiştir. Bu NP’lerin karakterizasyonunda XRD ve SEM analizleri kullanılmıştır. Ayrıca, yeşil sentezlenen MgO NP’lerin antibakteriyel aktivitesini belirlemek amacıyla çeşitli bakteri suşlarına (E. faecalis, Y. enterocolitica, E. coli, S. aureus, P. aeruginosa ve A. baumannii) karşı etkileri değerlendirilmiştir. XRD verileri, yeşil sentezlenen MgO NP’lerin polikristalin kübik yapıda olduğunu göstermiştir. SPE ve TSE kullanılarak üretilen MgO NP’lerin SEM görüntüleri, sırasıyla pürüzsüz yüzeyli, yaklaşık küresel ve hilal benzeri morfolojilere sahip olduklarını göstermiştir. SPE ve TSE ekstraktları kullanılarak üretilen MgO nanopartiküllerinin sırasıyla yaklaşık 80 ve 250 nm olmak üzere belirgin biçimde farklı boyutlara sahip olduğu belirlenmiştir. Ayrıca, test edilen suşların antibakteriyel yanıtlarının kullanılan bitki ekstraktına bağlı olarak değiştiği; en yüksek inhibisyon zonlarının TSE kökenli MgO NP’lerde A. baumannii’ye karşı 13,0 ± 1,0 mm ve SPE kökenli MgO NP’lerde E. faecalis’e karşı 9,0 ± 0,5 mm olarak gözlendiği belirlenmiştir. Bu çalışma, Türkiye’nin Doğu Akdeniz Bölgesi’nde yer alan Osmaniye ilinden toplanan S. perfoliata ve T. spicata var. spicata ekstraktları kullanılarak yeşil sentezlenen MgO NP’lerin antibakteriyel aktivitesine ilişkin ilk rapordur.

Keywords

• Antibakteriyel
• nanopartikül
• Sideritis
• Thymbra
• yeşil sentez

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