Phenolic Content of Some Dietetic Tea Products in Turkey

Year 2017, , 350 - 354, 24.12.2017

Hale Secilmis Canbay , Mahmut Doganturk

https://doi.org/10.24323/akademik-gida.370099

Abstract

High performance liquid chromatography (HPLC) system was used
to determine phenolic contents of dietetic tea
samples (n=10) marketed in Turkey. The most abundant phenolic compounds were
gallic acid (ND–6.98 mg/100 g dw), protocatechuic
acid (0.98–45.61 mg/100 g dw), 4-hydroxybenzoic
acid (ND–31.56 mg/100 g dw), chlorogenic
acid (ND–0.73 mg/100 g dw), caffeic acid (11.27–154.55
mg/100 g dw), p-coumaric acid
(ND–6.56 mg/100 g dw), ferulic acid (ND–1.13 mg/100 g dw), ellagic acid
(0.10–1.76 mg/100 g dw), epicatechin (7.59–290.07 mg/100 g dw), rutin (ND–49.95
mg/100 g dw) and quercetin (1.15–10.50 mg/100 g dw). Based on the results of
this study, sensitivity and accuracy were good. Calibration curves had a good
linearity for all compounds (r²>0.999). The quantitation limit
ranged 0.010 to 0.050 µg/g in dietetic tea
products. Recoveries were in the range of 86.45-97.35%. Results had good
accuracy and reproducibility.

Keywords

Phenolic compound, Dietetic tea, HPLC

Türkiye’deki Bazı Diyet Çay Örneklerinin Fenolik Bileşik İçeriği

Abstract

Türkiye’de satılan on diyet çay örneklerinin (n=10)
fenolik bileşik içerikleri, yüksek performanslı sıvı kromatografisi (HPLC)
sistemi ile belirlenmiştir. En çok bulunan fenolik bileşikler, gallik asit
(ND-6.98 mg/100 g kuru numune), protokateşik asit (0.98-45.61 mg/100 g kuru
numune), 4-hidroksibenzoik asit (ND-31.56 mg/100 g kuru numune), klorojenik
asit (ND-0.73 mg/100 g kuru numune), kafeik asit (11.27-154.55 mg/100 g kuru
numune), p-kumarik asit (ND-6.56
mg/100 g kuru numune), ferulik asit (ND–1.13 mg/100 g dw), ellajik asit (0.10-1.76 mg/100 g kuru numune), epikateşin (7.59-290.07
mg/100 g kuru numune), rutin (ND-49.95 mg/100 g kuru numune) ve kuersetindir
(1.00 mg/100 g kuru numune). Elde edilen sonuçlara göre, hassasiyet ve doğruluk
iyi bulunmuştur. Kalibrasyon eğrileri, tüm bileşikler için iyi bir doğrusallık
göstermiştir (r²> 0.999). Diyet çay ürünlerinde tespit limiti,
0.010-0.050 μg/g arasında değişmiştir. Geri kazanımlar %86.45-97.35 aralığındadır.
Bu çalışmada elde edilen sonuçların doğruluk ve tekrarlanabilirliklerinin iyi
olduğu belirlenmiştir.

Keywords

Fenolik bileşik, Diyet çay, HPLC

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