Monascus purpureus’dan Katı Kültür Fermantasyon Yöntemi ile Renk Pigmenti Üretimi

Year 2021, , 150 - 158, 01.08.2021

Yekta Göksungur Seda Sarıyıldız

https://doi.org/10.24323/akademik-gida.977272

Abstract

Bu araştırmada katı kültür fermantasyon tekniği ile Monascus purpureus CMU 001 küfünden mikrobiyal pigment üretimi gerçekleştirilmiştir. Substrat olarak bira mayşe atığı ve gofret yaprağı tozu kullanılmış ve en yüksek kırmızı renk pigmenti sentezinin gerçekleştiği fermantasyon koşulları belirlenmiştir. En yüksek pigment üretimi olan 59.55 AU500/g değeri, ilave besin elementi kullanılmadan 1:4 (g/g) oranında karıştırılmış bira mayşe atığı ve atık gofret yaprağı tozu substrat olarak kullanıldığında, optimum koşullar olan %85 başlangıç nem oranında, başlangıç pH 7.5 değerinde, 3 mL aşı hacmi ve 28°C inkübasyon sıcaklığında, fermantasyonun 9. gününde elde edilmiştir. Bu çalışma proses atıkları olan bira mayşe atığı ve gofret yaprağı tozunun katı kültür fermantasyon tekniği ile renk pigmenti üretiminde beraber kullanıldığı ilk çalışmadır.

Keywords

Monascus, Pigment, Katı kültür fermantasyonu, Bira mayşe atığı, Atık gofret yaprağı, Atık değerlendirme

Supporting Institution

Ege Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

FYL-2019-21067

Thanks

Bu çalışma Ege Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından desteklenmiştir (Proje No: FYL-2019-21067). Çalışma kapsamında kullanılan gofret yapraklarını temin eden Tören Gıda Sanayi ve Ticaret A.Ş. ve bira mayşe atığını temin eden Türk Tuborg Bira ve Malt Sanayi A.Ş.’ye çalışmaya verdikleri desteklerden ötürü teşekkür ederiz.

Production of Microbial Pigments by Solid-State Fermentation Technique using Monascus purpureus

Abstract

In this study, microbial pigment was produced by Monascus purpureus CMU 001 by using the solid-state fermentation technique. Brewer’s spent grain and waste wafer sheets were used as a substrate, and process parameters for the best pigment yield were determined. Brewer’s spent grain and waste wafer sheets mixed at a ratio of 1:4 (w/w) gave the highest pigment yield values. Extra nutrients were not used in the fermentation medium. The highest pigment concentration of 59.55 AU500/g was obtained under the optimized process conditions of initial moisture content 85%, initial pH 7.5, inoculum size 3 mL, fermentation temperature 28°C and incubation period 9 days. This was the first study on the use of brewer’s spent grain and waste wafer sheet in microbial pigment production using the solid-state fermentation technique.

Keywords

Monascus, Pigments, Solid-state fermentation, Brewers’ spent grain, Waste wafer sheet, Waste evaluation

Project Number

FYL-2019-21067

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