Inactivation Kinetics of Pectin Methyl Esterase under Thermosonication and Thermal Pasteurisation Process Conditions in Orange Juice

Year 2016, Volume: 14 Issue: 2, 85 - 91, 01.06.2016

Erdal Agcam Suleyman Polat Asiye Akyildiz Hasan Fenercioglu

Abstract

Pectin methylesterase has a higher thermal resistance than the microorganisms existing in orange juice, and its inactivation is used as a parameter for the pasteurisation process. In this study, the thermal and thermal-ultrasound thermosonication inactivation kinetics were determined. Freshly squeezed orange juice Valencia was heated at three temperatures 65, 75 and 85°C or with ultrasound 35 kHz/300 W ; four holding times 15, 30, 60 and 120 s were used for each condition, using laboratory type continuous heat exchanger. The inactivation of pectin methylesterase was influenced by ultrasound and processing temperature. A slower reaction rate constant was found for juices with only heat treatment at the studied temperatures. The highest inactivation level was obtained in juices with combined heat and ultrasound treatment at 85°C. Results indicated that the activation energy for inactivation of pectin methylesterase can be decreased 62% by the combined heat and ultrasound treatments

Keywords

Pectin methylesterase inactivation, Thermosonication, Kinetics, Orange juice

Portakal Suyundaki Pektin Metil Esterazın Termosonikasyon ve Isıl Pastörizasyon İşlem Koşullarında İnaktivasyon Kinetiği

Abstract

Pektin metil esteraz PME portakal suyunda var olan mikroorganizmalardan daha yüksek ısıl dirence sahiptir ve bundan dolayı portakal suyunun pastörizasyonunda, PME inaktivasyonu ısıl işlem normu olarak kullanılır. Bu çalışmada ısıl işlem veya ısıl işlem+ultrason termosonikasyon uygulamaları ile pektin metil esterazın inaktivasyon kinetikleri değerlendirilmiştir. Çalışmada taze sıkılmış portakal suyu Valencia 3 farklı sıcaklıkta 65, 75, 85°C her bir sıcaklık için 4 farklı sürede 15, 30, 60, 120 s ısıl pastörizasyon veya aynı sıcaklık ve sürelerde ultrason 35 kHz/300 W uygulaması gerçekleştirilmiştir. Pektin metil esterazın inaktivasyonunda ultrason ve işlem sıcaklığının etkili olduğu tespit edilmiştir. Sadece ısıl işlem uygulanan örneklerde daha düşük inaktivasyon hız sabiti belirlenmiştir. En yüksek inaktivasyon seviyeleri 85 °C' de sıcaklık ve ultrason termosonikasyon destekli uygulamadan elde edilmiştir. Sonuçlar, pektin metil esteraz inaktivasyonu için gerekli olan aktivasyon enerjisi değerinin termosonikasyon işlemiyle % 62 oranında azaltılabileceğini göstermiştir

Keywords

Pektin metil esteraz inaktivasyonu, Termosonikasyon, Kinetik, Portakal suyu

References

• FAS (United States Department of Agriculture, Foreign http://www.fas.usda.gov/htp/2011_jan_citrus.Pdf). (Accessed on 27.12.12). Service).
• Baker, R.A., Cameron, R.G., 1999. Clouds of citrus juices and juice drinks. Food Technology 53(1): 64–69.
• Basak, S., Ramaswamy, H.S., Simpson, B.K., 2001. High pressure inactivation of pectin methyl esterase in orange juice using combination treatments. Journal of Food Biochemistry 25(6): 509–526.
• Van Den Broeck, I., Ludikhuyze, L.R., Van Loey, A.M., Hendrickx, M.E., 2000. Inactivation of orange pectinesterase by combined high pressure and temperature treatments: a kinetic study. Journal of Agricultural and Food Chemistry 48(5): 1960–1970.
• Kimball, D.A., 1991. Citrus processing, Quality control and technology, (pp. 117–125), Van Nostrand Reinhold, New York, USA.
• Marshall, M.R., Marcy, J.E., Braddock, R.J., 1985. Effects of total solids level on heat inactivation of pectinesterase in orange juice. Journal of Food Science 50: 220-222.
• Polydera, A.C., Galanou, E., Stoforos, N.G, Taoukis, P.S., 2004. Inactivation of pectin methylesterase of greek Navel orange juice as a function temperature process conditions. Journal of Food Engineering 62: 291-298. pressure and
• Chen, C.S., Wu, M.C., 1998. Kinetic models for thermal inactivation of multiple pectinesterase in citrus juice. Journal of Food Science 63(5): 747- 750.
• Eagerman, B.A., Rouse, A.H., 1976. Heat inactivation tempurature-time relationships for pectinesterase inactivation in citrus juices. Journal of Food Science 41: 1396-1397.
• Espachs-Barroso, A., Van Loey, A., Hendrickx, M., Martin-Belloso, O., 2006. Inactivation of plant pectin methylesterase by thermal or high intensity pulsed electric field treatments. Innovative Food Science and Emerging Technologies 7: 40–48.
• Collet, L.S.F.C.A., Shigeoka, D.S., Badolato, G.G., Tadini, pectinesterase pasteurization of orange juice. Journal of Food Engineering 69: 125-129. kinetic during continous [12] Irwe, S., Olsson, I., 1994. Reduction of
• pectinesterase activity in orange juice by high
• pressure treatment, In: Singh, R.P., Olivera, F.A.
• editors, Minimal processing of foods and process
• optimization, Ann Arbor: CRC pres. p 35-42.
• Min, S., Jin, Z.T., Min, S.K., Yeom, H., Zhang, Q.H., 2003. Commercial-scale pulsed electric field processing of orange juice. Journal of Food Science 68(4): 1265–1271.
• Torregrosa, F., Cortés, C., Esteve, C.M., Frígola, A., 2005. Effect of high-intensity pulsed electric fields processing and conventional heat treatment on orange-carrot juice carotenoids. Journal of Agricultural and Food Chemistry 53: 9519-9525.
• Rivas, A., Rodrigo, D., Barbosa-Cánovas, G.V., Martínez, A., Rodrigo, M., 2006. Effect of PEF and heat pasteurization on the physical-chemical characteristics of blended orange and carrot juice. LWT–Food Science Technology 39(10): 1163– 1170.
• Polydera, A.C., Stoforos, N.G, Taoukis, P.S., 2005. Quality degradation kinetics of pasteurized and high pressure processed fresh Navel orange juice: Nutritional parameters and shelf life. Innovative Food Science and Emerging Technologies 6: 1-9.
• Odriozola-Serrano, I., Soliva-Fortuny, R., Martin- Belloso, O., 2008. Phenolic acids, flavonoids, vitamin C and antioxidant capacity of strawberry juices processed by high-intensity pulsed electric fields or heat treatments. European Food Research and Technology 228: 239-248.
• Vervoort, L., Van der Plancken, I., Grauwet, T., Timmermans, R.A.H., Mastwijk, H.C., Matser, A.M., Hendrickx, M. E., Van Loey, A., 2011. Comparing equivalent thermal, high pressure and pulsed electric field processes for mild pasteurization of orange juice. Part II: Impact on specific chemical and biochemical quality parameters. Innovative Food Science and Emerging Technologies 12(4): 466-477.
• Odriozola-Serrano, I.
• Soliva-Fortuny, R.
• Hernández-Jover, T., Martín-Belloso, O., 2009.
• Carotenoid and phenolic profile of tomato juices
• processed by high intensity pulsed electric fields
• compared with conventional thermal treatments.
• Food Chemistry 112: 258-266.
• Nienaber, U., Shellhammer, T.H., 2001. High- pressure procesing of orange juice: Kinetics of pectinmethylesterase Engineering and Physical Properties 66(2): 328- 331. inactivation. Food
• Bull, M.K., Zerdin, K., Howe, E., Goicaechea, D., Paramanandhan, P., Stockman, R., Sellahewa, J., Szabo, E.A., Johnson, R.L., Stewart, C.M., 2004. The effect of high pressure processing on the microbial, physical and chemical properties of Valencia and Navel orange juice. Innovative Food Science and Emerging Technologies 5: 135-149.
• Elez-Martínez, P., Suárez-Recio, M., Martín- Belloso, O., 2007. Modelling the reduction of pectin methyl esterase activity in orange juice by high intensity pulsed electric fields. Journal of Food Engineering 78: 184-193.
• Yeom, H.W., Streaker, C.B., Zhang, Q.H., Min, D.B., 2000. Effects of pulsed electric fields on the activities of microorganismis and pectin methyl esterase in orange juice. Journal of Food Science 65(8): 1359-1363.
• Zhang, R., Cheng, L., Wang, L., Guan, Z., 2006. Inactivation effects of PEF on horseradish peroxidase (HRP) and pectinesterase (PE). IEEE Transactions on Plasma Science 34(6): 2630-2636.
• Giner, J., Gimeno, V., Espachs, A., Elez, P., Barbosa- Cánovas, G., Martin, O., 2000. Inhibition of tomato (Licopersicon esculentum Mill) pectin methylesterase by pulsed electric fields. Innovative Food Science and Emerging Technologies 1: 57– 67.
• Tiwari, B.K., O’Donnell, C.P., Muthukumarappan, K., Cullen, P.J., 2009. Effect of sonication on orange juice quality parameters during storage. International Journal of Food Science and Technology 44(3): 586-595.
• Raviyan, P., Zhang, Z., Feng, H., 2005. Ultrasonication for tomato pectinmethylesterase inactivation: effect of cavitation intensity and temperature on inactivation. Journal of Food Engineering 70(2): 189-196.
• Sampedro, F., Rodrigo, D., Hendrickx, M., 2008. Inactivation kinetics of pectin methyl esterase under combined thermal-high pressure treatment in an orange juice-milk beverage. Journal of Food Engineering 86: 133-139.
• Kuldiloke, J., 2002. Effect temperature and pressure treatments on enzyme activity and quality indicators of fruit and vegetable juices, Doctoral dissertation, Technical University of Berlin.
• Chemat, F., Huma, Z., Khan, M.K., 2011. Applications of ultrasound in food technology: Processing, Ultrasonics Sonochemistry 18: 813–835. and extraction.
• Elez-Martínez, P., Soliva-Fortuny, R.C., Martín- Belloso, O., 2006. Comparative study on shelf life of orange juice processed by high intensity pulsed electric fields or heat treatment. European Food Research and Technology 222: 321–329.
• Vercet, A., Lopez, P., Burgos, J., 1999. Inactivation of heat-resistant pectinmethylesterase from orange by manothermosonication. Journal of Agriculture and Food Chemistry 47: 432-437.
• Vercet, A., Burgos, J., Crelier, S., Lopez-Buesa, P., 2001. Inactivation of proteases and lipases by ultrasound. Innovative Food Science and Emerging Technologies 2: 139-150.
• Valero, M., Recrosio, N., Saura, D., Munoz, N., Martic, N., Lizama, V., 2007. Effects of ultrasonic treatments in orange juice processing. Journal of Food Engineering 80: 509-516.
• Cameron, R.G., Niedz, R.P., Grohmann, K., 1994. Variable heat stability for multiple forms of pectin methylesterase from citrus tissue culture cells. Journal of Agriculture and Food Chemistry 42: 903- 908.
• Tajchakavit, S., Ramaswamy, H.S., 1997. Thermal vs microwave inactivation kinetics of pectin methylesterase in orange juice under batch mode heating conditions. LWT 30(1): 85-93.
• Terefe, N.S., Gamage, M., Vilkhu, K., Simons, L., Mawson, R., Versteeg, C., 2009. The kinetics of inactivation polygalacturonase thermosonication. Food Chemistry 117(1): 20-27. tomato by juice
• Wu, J., Gamage, T.V., Vilkhu, K.S., Simons, L.K., Mawson, R., 2008. Effect of thermosonication on quality improvement of tomato juice. Innovative Food Science & Emerging Technologies 9(2): 186- 195.
• Lopez, P., Vercet, A., Sanchez, A.C., Burgos, J., 1998. Inactivation of tomato pectic enzymes by manothermosonication. Z Lebensm Unters Forsch A. 207: 249-252.