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Chitosan and Chitosan Based Edible Film Applications

Year 2015, Volume: 13 Issue: 4, 348 - 353, 01.12.2015

Abstract

Chitosan is a natural carbohydrate polymer obtained by deacetylation of chitin, which is the second most abundant polysaccharide in nature. Considerable interest towards chitosan as a new functional material in various fields has been increased in recent years. This review summarizes some of the chemical and important properties of chitosan, production methods and its utilization as edible films in food industry. Chitosan films have shown potential to be used as packaging material for the preservation of qualities and improving storage times of variety foods because of its film forming and barrier properties. Studies have indicated that using chitosan as edible film provides advantages in the extension of the shelf life and preservation of food quality because of its antioxidant and antimicrobial properties

References

  • No, H.K., Meyers S.P., 1995. Preparation and characterization of chitin and chitosan—a Review. Journal of Aquatic Food Product Technology 4(2): 27-52.
  • Dutta, P.K., Dutta, J., Tripathi, V.S., 2004. Chitin and chitosan: applications. Journal of Scientific & Industrial Research 63: 20-31. properties and
  • Shahidi, F., Arachchi, J.K.V., Jeon, Y., 1999. Food applications of chitin and chitosans. Trends in Food Science & Technology 10: 37 – 51.
  • Agullo´, E., Rodriguez, M., Ramos, V., Albertengo, L., 2003. Present and future role of chitin and chitosan in food. Macromoleculer Bioscience 3: 521-530.
  • Debeaufort, F., Quezada-Gallo, J. A., Voilley, A., 1998. Edible films and coatings: tomorrow’s packagings: A Review. Critical Reviews in Food Science 38(4): 299–313.
  • Dutta, P.K., Tripathi, S., Mehrotra, G.K., Dutta, J., 2009. Perspectives for chitosan based antimicrobial films in food applications. Food Chemistry 114: 1173–1182.
  • Baldwin, E.A., Hagenmaier, R., Bai, J., 2009. Edible coatings and films to improve food quality. In: Coatings for fresh fruits and vegetables (Eds.: Bai, J., Plotto, A.), pp.: 186- 226.
  • Kim, I.Y., Seo, S.J., Moon, H.S., Yoo, M.K., Park, I.Y., Kim, B.C., Cho, C.S., 2008. Chitosan and its derivatives for tissue engineering applications. Biotechnology Advances 26: 1–21.
  • Kurita, K., 2006. Chitin and chitosan: functional biopolymers from marine crustaceans. Marine Biotechnology 8: 203-226.
  • Kumar, M.N.V.R., 2000. A review of chitin and chitosan applications effects of chitosan, calcium chloride, and pullulan coating treatments on antioxidant activity in pear. Reactive & Functional Polymers 46: 1–27.
  • Sagheer, F.A. A., Sughayer, M.A. A., Muslim, S., Elsabee, M.Z., 2009. Extraction and characterization of chitin and chitosan from marine sources in Arabian Gulf. Carbohydrate Polymers 77: 410–419.
  • Jeon, Y.J., Kamil, Y.V.A.J., Shahidi, F., 2002. Chitosan as an edible invisible film for quality preservation of herring and Atlantic cod. Journal of Agricultural and Food Chemistry 50: 5167-5178.
  • Thirunavukkarasu, N., Shanmugam. A., 2009. Extraction of chitin and chitosan from mud crab Scylla tranquebarica (Fabricius, 1798). International Journal on Applied Bioengineering 4(2): 31-33.
  • Yen, M.T., Yang, J.H., Mau, J.L., 2009. Physicochemical characterization of chitin and chitosan from crab shells. Carbohydrate Polymers 75: 15–21.
  • Jothi, N., Nachiyar, R.K., 2012. Identification and isolation of chitin and chitosan from cuttlebone of Sepla prashadi Winckworth, 1936. Current Biotica 6(3): 304-313.
  • Kafetzopoulos, D., Martinou, A., Bouriotis, V., 1993. Bioconversion of chitin to chitosan: Purification and characterization of chitin deacetylase from Mucor rouxii. Applied Biological Sciences 90: 2564-2568.
  • Raval, R., Raval, K., Moerschbacher B. M., 2013. Enzymatic modification of chitosan using chitin deacetylase isolated from Bacillus cereus. Open Access Scientific Reports 2(1): 1-4.
  • Agboh, O.C., Qin, Y., 1997. Chitin and chitosan fibers. Polymers for Advanced Technologies 8: 355–365.
  • Kaur, S., Dhillon, G. S., 2013. Recent trends in biological extraction of chitin from marine shell wastes: a review. Crit Rev Biotechnol. Early Online: 1–18.
  • Kim, S.O.F., 2004. Physicochemical and functional properties of crawfish chitosan as affected by different processing protocols. (Thesis), B.S., Seoul National University.
  • Kandra, P., Challa, M. M., Jyothi, H. K. P., 2012. Efficient use of shrimp waste: present and future trends. App. Microbiol. Biotechnol. 93: 17–29.
  • Gagne, N., 1993. Production of chitin and chitosan from crustacean waste and their use as a food processing aid. (Thesis), McGill University, Montreal.
  • Cai, J., Yang, J., Du, Y., Fan, L., Qiu, Y., Li, J., Kennedy, J.F., 2006. Enzymatic preparation of chitosan from the waste Aspergillus niger mycelium of citric acid production plant. Carbohydrate Polymers 64: 151–157.
  • Guerard, F., Sumaya-Martinez, M.T, Laroque, D., Chabeaud, A., Dufosse, L., 2007. Optimization of free radical scavenging activity by response surface methodology in the hydrolysis of shrimp processing discards. Process Biochem. 42: 1486–1491.
  • Mizani, A. M., Aminlari, B.M., 2007. A new process for deproteinization of chitin from shrimp head waste. Proceedings of European Congress of Chemical Engineering, Copenhagen. (ECCE-6): 16–20.
  • Sini, T.K., Santhosh, S., Mathew, P.T., 2007. Study on the production of chitin and chitosan from shrimp shell by using Bacillus subtilis fermentation. Carbohydrate Research 342: 2423–2429.
  • White, S.A., Farina, P.R., Fulton, I., 1979. Production and isolation of chitosan from Mucor rouxii. Applied and Environmental Microbiology 38(2): 323-328.
  • Nadarajah, K., Kader, J., Mazmira, M., Paul, D.C., 2001. Production of chitosan by fungi. Pakistan Journal of Biological Sciences 4(3): 263-265.
  • Suntornsuk, W., Pochanavanich, P., Suntornsuk, L., 2002. Fungal chitosan production on food processing by-products. Process Biochemistry 37: 727–729.
  • Kittur, F.S., Kumar, K.R.,Tharanathan, R.N., 1998. Functional packaging properties of chitosan films. Z Lebensm Unters Forsch A. 206: 44-47.
  • Vargas, M., Albors, A., Chiralt, A., Gonzalez- Martinez, C., 2006. Quality of cold-stored strawberries as affected by chitosan–oleic acid edible Technology 41: 164–171. Biology and
  • Hong, K., Xie, J., Zhang, L., Sun, D., Gong, D., 2012. Effects of chitosan coating on postharvest life and quality of guava (Psidium guajava L.) fruit during cold storage. Scientia Horticulturae 144: 172–178.
  • Ghasemnezhad, M., Zareh, S., Rassa, M., Sajedi, R.H., 2013. Effect of chitosan coating on maintenance of aril quality, microbial population and PPO activity of pomegranate (Punica granatum L. cv. Tarom) at cold storage temperature. J Sci Food Agric. 93: 368–374.
  • Han, C., Zuoa, J., Wanga, Q., Xua, L., Zhaia, B., Wang, Z., Dong, H., Gao, L., 2014. Effects of chitosan coating on postharvest quality and shelf life of sponge gourd (Luffa cylindrica) during storage. Scientia Horticulturae 166: 1–8.
  • Chung, Y., Su, Y., Chen, C., Jia, G., Wang, H., Wu, J.C.G., Lin, J., 2004. Relationship between antibacterial activity of chitosan and surface characteristics of cell wall. Acta Pharmacol Sin. 25(7): 932-936.
  • Zivanovic, S., Chi, S., Draughon, A.F., 2005. Antimicrobial activity of chitosan films enriched with essential oils. Food Microbiology and Safety 70(1): 45-51.
  • Jeihanipour, A., Karimi, K., Taherzadeh, M.J., 2007. Antimicrobial properties of fungal chitosan. Research Journal of Biological Sciences 2(3): 239- 243.
  • Campaniello, D., Bevilacqua, A., Sinigaglia, M., Corbo, M.R., 2008. Chitosan: Antimicrobial activity and potential applications for preserving minimally processed strawberries. Food Microbiology 25: 992–1000.
  • Moreira, M.R., Roura, S.I., Ponce, A., 2011. Effectiveness of chitosan edible coatings to improve microbiological and sensory quality of fresh cut broccoli. Food Science and Technology 44: 2335- 2341.
  • Pizato, S., Cortez-Vega, W.R., De Souza, J.T.A., Prentıce-Hernández, C., Borges, C.D., 2013. Effects of different edible coatings in physical, chemical and microbiological characteristics of minimally processed peaches (Prunus Persıca L. Batsch). Journal of Food Safety 33: 30–39.
  • Rabea, E.I., Badawy, M.E.T., Stevens, C.V., Smagghe, G., Steurbaut, W., 2003. Chitosan as antimicrobial agent: applications and mode of action. Biomacromolecules, 4(6): 1457-1465.
  • No, H.K., Meyers, S.P., Prinyawiwatkul, W., Xu, Z., 2007. Applications of chitosan for improvement of quality and shelf life of foods: A Review. Journal of Food Science 72(5): 87-100.
  • Aider, M., 2010. Chitosan application for active bio- based films production and potential in the food industry: Review. Food Science and Technology 43: 837–842.
  • Kamil, J.Y.V.A., Jeon, Y.J., Shahidi, F., 2002. Antioxidative activity of chitosans of different viscosity in cooked comminuted flesh of herring (Clupea harengus). Food Chemistry 79: 69–77.
  • Kim, K.W., Thomas, R.L., 2007. Antioxidative activity of chitosans with varying molecular weights. Food Chemistry 101: 308–313.
  • Shahidi, F., Kamil, J., Jeon, Y.J., Kim, S.K., 2002. Antioxidant role of chitosan in cooked cod (Godus morhua) model system. Journal of Food Lipids 9(1): 57–64.
  • Geraldine, R.M., Soares, N.F.F., Botrel, D.A., Goncalves, L.A., 2008. Characterization and effect of edible coatings on minimally processed garlic quality. Carbohydrate Polymers 72: 403–409.
  • Dang, Q., Yan, J., Li, Y., Cheng, X.J., Liu, C.S., Chen, X.G., 2010. Chitosan acetate as an active coating material and its effects on the storing of Prunus Avium L. Journal of Food Science 75(2): 125-131.
  • Ghasemnezhad, M., Shiri, M.A., Sanavi, M., 2010. Effect of chitosan coatings on some quality indices of apricot (Prunus armeniaca L.) during cold storage. Caspian J. Env. Sci. 8(1): 25-33.
  • Shiri, M. A., Bakhshi, D., Ghasemnezhad, M., Dadi, M., Papachatzis,, A., Kalorizou, H., 2013. Chitosan coating improves the shelf life and postharvest quality of table grape (Vitis vinifera) cultivar Shahroudi. Turkish Journal of Agriculture and Forestry 37: 148-156.
  • Kou, X.H., Guo, W., Guo, R., Li, X., Xue, Z., 2014. cv. Effects of chitosan, calcium chloride, and pullulan coating treatments on antioxidant activity in pear cv. “Huang guan” during storage. Food Bioprocess Technol. 7: 671–681.

Kitosan ve Kitosan Bazlı Yenilebilir Film Uygulamaları

Year 2015, Volume: 13 Issue: 4, 348 - 353, 01.12.2015

Abstract

Kitosan, doğada en çok bulunan ikinci polisakkarit olan kitinin deasetilasyonuyla elde edilen doğal bir karbonhidrat polimeridir. Çeşitli alanlarda kullanılabilen yeni bir fonksiyonel materyal olmasından dolayı kitosana olan ilgi son yıllarda önemli düzeyde artmıştır. Bu derlemede kitosanın kimyasal yapısı, özellikleri, üretim yöntemleri ve gıdalarda yenilebilir film olarak kullanımı özetlenmiştir. Kitosan film oluşturma ve bariyer özelliklerinin iyi olması sebebiyle çeşitli gıdaların kalitelerinin korunması ve depolama sürelerinin artırılmasında ambalajlama materyali olarak kullanım potansiyeline sahiptir. Yapılan çalışmalar kitosanın antioksidan ve antimikrobiyal özellikleri nedeniyle yenilebilir film olarak kullanımının gıdaların kalitelerinin korunması ve raf ömürlerinin uzatılmasında avantaj sağlayacağını göstermektedir

References

  • No, H.K., Meyers S.P., 1995. Preparation and characterization of chitin and chitosan—a Review. Journal of Aquatic Food Product Technology 4(2): 27-52.
  • Dutta, P.K., Dutta, J., Tripathi, V.S., 2004. Chitin and chitosan: applications. Journal of Scientific & Industrial Research 63: 20-31. properties and
  • Shahidi, F., Arachchi, J.K.V., Jeon, Y., 1999. Food applications of chitin and chitosans. Trends in Food Science & Technology 10: 37 – 51.
  • Agullo´, E., Rodriguez, M., Ramos, V., Albertengo, L., 2003. Present and future role of chitin and chitosan in food. Macromoleculer Bioscience 3: 521-530.
  • Debeaufort, F., Quezada-Gallo, J. A., Voilley, A., 1998. Edible films and coatings: tomorrow’s packagings: A Review. Critical Reviews in Food Science 38(4): 299–313.
  • Dutta, P.K., Tripathi, S., Mehrotra, G.K., Dutta, J., 2009. Perspectives for chitosan based antimicrobial films in food applications. Food Chemistry 114: 1173–1182.
  • Baldwin, E.A., Hagenmaier, R., Bai, J., 2009. Edible coatings and films to improve food quality. In: Coatings for fresh fruits and vegetables (Eds.: Bai, J., Plotto, A.), pp.: 186- 226.
  • Kim, I.Y., Seo, S.J., Moon, H.S., Yoo, M.K., Park, I.Y., Kim, B.C., Cho, C.S., 2008. Chitosan and its derivatives for tissue engineering applications. Biotechnology Advances 26: 1–21.
  • Kurita, K., 2006. Chitin and chitosan: functional biopolymers from marine crustaceans. Marine Biotechnology 8: 203-226.
  • Kumar, M.N.V.R., 2000. A review of chitin and chitosan applications effects of chitosan, calcium chloride, and pullulan coating treatments on antioxidant activity in pear. Reactive & Functional Polymers 46: 1–27.
  • Sagheer, F.A. A., Sughayer, M.A. A., Muslim, S., Elsabee, M.Z., 2009. Extraction and characterization of chitin and chitosan from marine sources in Arabian Gulf. Carbohydrate Polymers 77: 410–419.
  • Jeon, Y.J., Kamil, Y.V.A.J., Shahidi, F., 2002. Chitosan as an edible invisible film for quality preservation of herring and Atlantic cod. Journal of Agricultural and Food Chemistry 50: 5167-5178.
  • Thirunavukkarasu, N., Shanmugam. A., 2009. Extraction of chitin and chitosan from mud crab Scylla tranquebarica (Fabricius, 1798). International Journal on Applied Bioengineering 4(2): 31-33.
  • Yen, M.T., Yang, J.H., Mau, J.L., 2009. Physicochemical characterization of chitin and chitosan from crab shells. Carbohydrate Polymers 75: 15–21.
  • Jothi, N., Nachiyar, R.K., 2012. Identification and isolation of chitin and chitosan from cuttlebone of Sepla prashadi Winckworth, 1936. Current Biotica 6(3): 304-313.
  • Kafetzopoulos, D., Martinou, A., Bouriotis, V., 1993. Bioconversion of chitin to chitosan: Purification and characterization of chitin deacetylase from Mucor rouxii. Applied Biological Sciences 90: 2564-2568.
  • Raval, R., Raval, K., Moerschbacher B. M., 2013. Enzymatic modification of chitosan using chitin deacetylase isolated from Bacillus cereus. Open Access Scientific Reports 2(1): 1-4.
  • Agboh, O.C., Qin, Y., 1997. Chitin and chitosan fibers. Polymers for Advanced Technologies 8: 355–365.
  • Kaur, S., Dhillon, G. S., 2013. Recent trends in biological extraction of chitin from marine shell wastes: a review. Crit Rev Biotechnol. Early Online: 1–18.
  • Kim, S.O.F., 2004. Physicochemical and functional properties of crawfish chitosan as affected by different processing protocols. (Thesis), B.S., Seoul National University.
  • Kandra, P., Challa, M. M., Jyothi, H. K. P., 2012. Efficient use of shrimp waste: present and future trends. App. Microbiol. Biotechnol. 93: 17–29.
  • Gagne, N., 1993. Production of chitin and chitosan from crustacean waste and their use as a food processing aid. (Thesis), McGill University, Montreal.
  • Cai, J., Yang, J., Du, Y., Fan, L., Qiu, Y., Li, J., Kennedy, J.F., 2006. Enzymatic preparation of chitosan from the waste Aspergillus niger mycelium of citric acid production plant. Carbohydrate Polymers 64: 151–157.
  • Guerard, F., Sumaya-Martinez, M.T, Laroque, D., Chabeaud, A., Dufosse, L., 2007. Optimization of free radical scavenging activity by response surface methodology in the hydrolysis of shrimp processing discards. Process Biochem. 42: 1486–1491.
  • Mizani, A. M., Aminlari, B.M., 2007. A new process for deproteinization of chitin from shrimp head waste. Proceedings of European Congress of Chemical Engineering, Copenhagen. (ECCE-6): 16–20.
  • Sini, T.K., Santhosh, S., Mathew, P.T., 2007. Study on the production of chitin and chitosan from shrimp shell by using Bacillus subtilis fermentation. Carbohydrate Research 342: 2423–2429.
  • White, S.A., Farina, P.R., Fulton, I., 1979. Production and isolation of chitosan from Mucor rouxii. Applied and Environmental Microbiology 38(2): 323-328.
  • Nadarajah, K., Kader, J., Mazmira, M., Paul, D.C., 2001. Production of chitosan by fungi. Pakistan Journal of Biological Sciences 4(3): 263-265.
  • Suntornsuk, W., Pochanavanich, P., Suntornsuk, L., 2002. Fungal chitosan production on food processing by-products. Process Biochemistry 37: 727–729.
  • Kittur, F.S., Kumar, K.R.,Tharanathan, R.N., 1998. Functional packaging properties of chitosan films. Z Lebensm Unters Forsch A. 206: 44-47.
  • Vargas, M., Albors, A., Chiralt, A., Gonzalez- Martinez, C., 2006. Quality of cold-stored strawberries as affected by chitosan–oleic acid edible Technology 41: 164–171. Biology and
  • Hong, K., Xie, J., Zhang, L., Sun, D., Gong, D., 2012. Effects of chitosan coating on postharvest life and quality of guava (Psidium guajava L.) fruit during cold storage. Scientia Horticulturae 144: 172–178.
  • Ghasemnezhad, M., Zareh, S., Rassa, M., Sajedi, R.H., 2013. Effect of chitosan coating on maintenance of aril quality, microbial population and PPO activity of pomegranate (Punica granatum L. cv. Tarom) at cold storage temperature. J Sci Food Agric. 93: 368–374.
  • Han, C., Zuoa, J., Wanga, Q., Xua, L., Zhaia, B., Wang, Z., Dong, H., Gao, L., 2014. Effects of chitosan coating on postharvest quality and shelf life of sponge gourd (Luffa cylindrica) during storage. Scientia Horticulturae 166: 1–8.
  • Chung, Y., Su, Y., Chen, C., Jia, G., Wang, H., Wu, J.C.G., Lin, J., 2004. Relationship between antibacterial activity of chitosan and surface characteristics of cell wall. Acta Pharmacol Sin. 25(7): 932-936.
  • Zivanovic, S., Chi, S., Draughon, A.F., 2005. Antimicrobial activity of chitosan films enriched with essential oils. Food Microbiology and Safety 70(1): 45-51.
  • Jeihanipour, A., Karimi, K., Taherzadeh, M.J., 2007. Antimicrobial properties of fungal chitosan. Research Journal of Biological Sciences 2(3): 239- 243.
  • Campaniello, D., Bevilacqua, A., Sinigaglia, M., Corbo, M.R., 2008. Chitosan: Antimicrobial activity and potential applications for preserving minimally processed strawberries. Food Microbiology 25: 992–1000.
  • Moreira, M.R., Roura, S.I., Ponce, A., 2011. Effectiveness of chitosan edible coatings to improve microbiological and sensory quality of fresh cut broccoli. Food Science and Technology 44: 2335- 2341.
  • Pizato, S., Cortez-Vega, W.R., De Souza, J.T.A., Prentıce-Hernández, C., Borges, C.D., 2013. Effects of different edible coatings in physical, chemical and microbiological characteristics of minimally processed peaches (Prunus Persıca L. Batsch). Journal of Food Safety 33: 30–39.
  • Rabea, E.I., Badawy, M.E.T., Stevens, C.V., Smagghe, G., Steurbaut, W., 2003. Chitosan as antimicrobial agent: applications and mode of action. Biomacromolecules, 4(6): 1457-1465.
  • No, H.K., Meyers, S.P., Prinyawiwatkul, W., Xu, Z., 2007. Applications of chitosan for improvement of quality and shelf life of foods: A Review. Journal of Food Science 72(5): 87-100.
  • Aider, M., 2010. Chitosan application for active bio- based films production and potential in the food industry: Review. Food Science and Technology 43: 837–842.
  • Kamil, J.Y.V.A., Jeon, Y.J., Shahidi, F., 2002. Antioxidative activity of chitosans of different viscosity in cooked comminuted flesh of herring (Clupea harengus). Food Chemistry 79: 69–77.
  • Kim, K.W., Thomas, R.L., 2007. Antioxidative activity of chitosans with varying molecular weights. Food Chemistry 101: 308–313.
  • Shahidi, F., Kamil, J., Jeon, Y.J., Kim, S.K., 2002. Antioxidant role of chitosan in cooked cod (Godus morhua) model system. Journal of Food Lipids 9(1): 57–64.
  • Geraldine, R.M., Soares, N.F.F., Botrel, D.A., Goncalves, L.A., 2008. Characterization and effect of edible coatings on minimally processed garlic quality. Carbohydrate Polymers 72: 403–409.
  • Dang, Q., Yan, J., Li, Y., Cheng, X.J., Liu, C.S., Chen, X.G., 2010. Chitosan acetate as an active coating material and its effects on the storing of Prunus Avium L. Journal of Food Science 75(2): 125-131.
  • Ghasemnezhad, M., Shiri, M.A., Sanavi, M., 2010. Effect of chitosan coatings on some quality indices of apricot (Prunus armeniaca L.) during cold storage. Caspian J. Env. Sci. 8(1): 25-33.
  • Shiri, M. A., Bakhshi, D., Ghasemnezhad, M., Dadi, M., Papachatzis,, A., Kalorizou, H., 2013. Chitosan coating improves the shelf life and postharvest quality of table grape (Vitis vinifera) cultivar Shahroudi. Turkish Journal of Agriculture and Forestry 37: 148-156.
  • Kou, X.H., Guo, W., Guo, R., Li, X., Xue, Z., 2014. cv. Effects of chitosan, calcium chloride, and pullulan coating treatments on antioxidant activity in pear cv. “Huang guan” during storage. Food Bioprocess Technol. 7: 671–681.
There are 51 citations in total.

Details

Primary Language Turkish
Journal Section Research Article
Authors

Kader Tokatlı This is me

Aslıhan Demirdöven This is me

Publication Date December 1, 2015
Published in Issue Year 2015 Volume: 13 Issue: 4

Cite

APA Tokatlı, K., & Demirdöven, A. (2015). Kitosan ve Kitosan Bazlı Yenilebilir Film Uygulamaları. Akademik Gıda, 13(4), 348-353.
AMA Tokatlı K, Demirdöven A. Kitosan ve Kitosan Bazlı Yenilebilir Film Uygulamaları. Akademik Gıda. December 2015;13(4):348-353.
Chicago Tokatlı, Kader, and Aslıhan Demirdöven. “Kitosan Ve Kitosan Bazlı Yenilebilir Film Uygulamaları”. Akademik Gıda 13, no. 4 (December 2015): 348-53.
EndNote Tokatlı K, Demirdöven A (December 1, 2015) Kitosan ve Kitosan Bazlı Yenilebilir Film Uygulamaları. Akademik Gıda 13 4 348–353.
IEEE K. Tokatlı and A. Demirdöven, “Kitosan ve Kitosan Bazlı Yenilebilir Film Uygulamaları”, Akademik Gıda, vol. 13, no. 4, pp. 348–353, 2015.
ISNAD Tokatlı, Kader - Demirdöven, Aslıhan. “Kitosan Ve Kitosan Bazlı Yenilebilir Film Uygulamaları”. Akademik Gıda 13/4 (December 2015), 348-353.
JAMA Tokatlı K, Demirdöven A. Kitosan ve Kitosan Bazlı Yenilebilir Film Uygulamaları. Akademik Gıda. 2015;13:348–353.
MLA Tokatlı, Kader and Aslıhan Demirdöven. “Kitosan Ve Kitosan Bazlı Yenilebilir Film Uygulamaları”. Akademik Gıda, vol. 13, no. 4, 2015, pp. 348-53.
Vancouver Tokatlı K, Demirdöven A. Kitosan ve Kitosan Bazlı Yenilebilir Film Uygulamaları. Akademik Gıda. 2015;13(4):348-53.

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