Effect of Different Cryoprotectants on Salmonella typhimurium during Freeze Drying

Year 2012, Volume: 10 Issue: 4, 28 - 30, 01.12.2012

Ahmet Koluman Sibel Özkök Zeynep T Burkan

Abstract

Freeze drying is a sophisticated way for long term storage of bacteria. Different chemicals and solutions are used to reach maximum recovery of strains after freeze-drying. Sucrose and skim milk are popular reagents that are massively used. Reagent 18 is widely used in different culture collections. In this study, different combinations of cryoprotectants are used for freeze-drying S. typhimurium, and stability of the strain was evaluated. The results showed that Reagent 18 is effective during the storage of freeze-dried S. typhimurium strains

Keywords

Salmonella, Freeze drying, Cryoprotectant, Reagent 18

Liyofilizasyonda Kullanılan Farklı Kriyoprotektantların Salmonella typhimurium Üzerine Etkisi

Abstract

Liyofilizasyon uzun süreli saklamada uygulanan bir yöntemdir. Farklı kimyasal ve çözeltiler liyofilizasyon sonrası suşların maksimum canlandırılması amacıyla kullanılmaktadır. Sakkaroz ve yağsız süt tozu en yaygın kullanılan ajanlardır. Reagent 18 genellikle farklı kültür koleksiyonlarında kullanılmaktadır. Bu araştırmada farklı kriyoprotektanlar hücre çekirdeğini soğuktan koruyan maddeler kullanılarak S. typhimurium liyofilize edilmiş ve suşun stabilitesi değerlendirilmiştir. Sonuçlar Reagent 18’in S. typhimurium suşlarının saklanmasında etkili olduğunu göstermiştir

Keywords

Salmonella, Liyofilizasyon, Kriyoprotektant, Reagent 18

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