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Determination of Some Quality Characteristics and Antioxidant Activities of UHT Milks

Year 2010, Volume: 8 Issue: 1, 13 - 16, 01.02.2010

Abstract

Total antioxidant activity and some quality properties were determined in UHT plain milk and chocolate- and strawberry-flavoured milk samples of six different brands. Total titratable acidity, pH, fat, total solids, total ash analysis, total phenolic content and total antioxidant activity using TEAC Trolox Equivalent Antioxidant Capacity, ABTS and ORAC Oxygen Radical Absorbance Capacity assays were determined in milk samples. Results indicated that total phenolic content, TEAC and ORAC values of strawberry-flavoured, chocolate-flavoured and plain milk samples ranged between 1152.93 ± 57.82, 1587.52 ± 229.84 and 1030.10 ± 19.31 mg gallic acid equivalents GAE /L, 5.38 ± 0.28, 6.25 ± 0.53 and 4.31 ± 0.51 mM Trolox equivalent, and 3.38 ± 0.36, 4.31 ± 0.57 and 2.98 ± 0.15 µmol Trolox equivalents/mL, respectively. The lowest total phenolic content, TEAC and ORAC values were determined in plain milk samples. The highest total antioxidant activity was determined in chocolate-flavoured milk samples p

References

  • AOAC 1997. Method 945.46, 947.05, 986.33, 989.04, 990.19. Official methods of analysis of AOAC international (16th ed.). Gaithersburg, MD 20877-2417. USA.
  • Benzie, I.F.F., 2003. Evolution of dietary antioxidants. Physiology Part A, 136:113-126. Biochemistry and
  • Çakatay, U., Kayalı, R., 2006. Serbest radikal biyokimyasının Cerrahpaşa Tıp Dergisi 37: 162-167. süreçteki gelişimi.
  • Çavdar, C., Sifil, A., Çamsarı, T., 1997. Reaktif oksijen partikülleri ve antioksidan savunma. Türk Nefroloji Diyaliz ve Transplantasyon Dergisi 3-4: 92- 95.
  • Cervato, G., Cazzola, R. & Cestaro, B., 1999. Studies on the antioxidant activity of milk caseins. International Journal of Food Sciences and Nutrition, 50: 291-296.
  • Clausen, M.R., Skibsted, L.H., Stagsted, J. 2009. Characterization of major radical scavenger species in chromatography and functional assays. Journal of Agricultural and Food Chemistry 57(7): 2912-2919.
  • Dávalos, A., Bartolomé, B., Gómez-Cordovés, C., 2004. Extending applicability of the oxygen radical absorbance capacity (ORAC-Fluorescein) assay. Journal of Agricultural and Food Chemistry 52: 48- 54.
  • Gu, L., House, S. E., Wu, X., Ou, B., & Prior, R. L. 2006. Procyanidin and catechin contents and antioxidant capacity of cocoa and chocholate products. Journal of Agricultural and Food Chemistry 54: 4057-4061.
  • Hudson, BJF., 1990. Food Antioxidants. Elsevier Applied Science Publishers, New York. Elsevier, New York, pp. 253–307.
  • Jiménez, A.M., Murcia, M.A., Parras, P., Martínez- Tomé, M. 2008. On the importance of adequately choosing the ingredients of yoghurt and enriched milk for their antioxidant activity. International Journal of Food Science and Technology. 43(8): 1464-1473.
  • O'Connel, J.E., and Fox, P.F. 2001. Significance and application of phenolic compounds in the production and quality of milk and dairy products: a review. International Dairy Journal 11(3):103-120.
  • Ostdal, H., Daneshvar, B., Skibsted, L., 1996. Reduction of ferrylmyoglobin by b-lactoglobulin. Free Radical Research 24: 429-438.
  • Pihlanto, A., 2006. Antioxidative peptides derives from milk proteins. International Dairy Journal 16, 1306-1314.
  • Piljac-Žegarac, J., Valek, L., Martinez, S., Belščak, A., 2009. Fluctuations in the phenolic content and antioxidant capacity of dark fruit juices in refrigerated storage. Food Chemistry 113: 394-400.
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C., 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine 26: 1231-1237.
  • Saenz, A.T., Elisia, I., Innis, S.M., Friel, J. K., Kitts, D. D. 2009. Use of ORAC to assess antioxidant capacity of human milk. Journal of Food Composition and Anaysis 22 (7-8): 694-698.
  • SAS Institute Inc., Version 8, Cary, NC, USA, 1999.
  • Shahidi, F. 2000. Antioxidants in Food and Food Antioxidants, Nahrung, 44,158-163.
  • Singleton, V.L., Orthofer, R., Lamuela-Raventos, R.M. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent. Methods in Enzymology 299: 152-178.
  • Steinberg, F. M., Bearden, M. M., Keen, C. L., 2003. Cocoa and chocolate flavonoids: Implications for cardiovascular health. Journal of the American Dietetic Association 103(2): 215-223.
  • Tong, L.M., Sasaki, S., McClements, D.J., Decker, E.A., 2000. Mechanisms of the antioxidant activity of a high molecular weight fraction of whey. Journal of Agriculture and Food Chemistry 48: 1473-1478.
  • TSE (Türk Standartları Enstitüsü) 2001. Uzun Ömürlü Süt Standardı. T.S. 1192. Ankara.
  • TSE (Türk Standartları Enstitüsü) 2002. Aromalı Süt Standardı. T.S. 5004. Ankara.
  • Tunalıer, Z., Öztürk, N., Koşar, M., Başer, K H C., Duman, H., Kırımer, N., 2002. Bazı sideritis türlerinin antioksidan etki ve fenolik bileşikler yönünden Hammaddeleri Toplantısı, 130-138, Eskişehir.
  • Yanishlieva, N V., Pokomy, J., Gordon, M., 2001. Bitkisel İlaç Inhibiting Oxidation in Antioxidants in Food: Practical Applications., CRC press LLC and Woodhead Publishing Ltd, New York, USA, 288s.
  • Zulueta, A., Esteve, M. J., Frasquet, I., Frígola, A., 2007. Vitamin C, vitamin A, phenolic compounds and total antioxidant capacity of new fruit juice and skim milk mixture beverages marketed in Spain. Food Chemistry 103: 1365-1374.

UHT Sütlerin Bazı Kalite Kriterlerinin ve Antioksidan Aktivitelerinin Belirlenmesi

Year 2010, Volume: 8 Issue: 1, 13 - 16, 01.02.2010

Abstract

Bu çalışmada sade ve aromalı süt örneklerinin antioksidan aktiviteleri ile bazı kimyasal özelliklerinin belirlenmesi amaçlanmıştır. Sade, çilek aromalı ve çikolatalı süt olmak üzere 6 farklı firmadan alınan karton kutu ambalajlı UHT sütler kullanılmıştır. Örneklerde titrasyon asitliği, pH, yağ, toplam kuru madde ve kül analizleri yapılmış olup, toplam fenolik madde içeriği ve toplam antioksidan aktiviteleri TEAC Troloks Eşdeğer Antioksidan Aktivitesi, ABTS ve ORAC Oksijen Radikal Absorbans Kapasitesi yöntemleriyle belirlenmiştir. Elde edilen sonuçlara göre çilek aromalı, çikolatalı ve sade sütlerde toplam fenolik madde içeriği sırasıyla 1152.93 ± 57.82, 1587.52 ± 229.84 ve 1030.10 ± 19.31 mg gallik asit eşdeğeri GAE /L, TEAC değerleri sırasıyla 5.38 ± 0.28, 6.25 ± 0.53 ve 4.31 ± 0.51 mM Troloks eşdeğeri, ORAC değerleri ise sırasıyla 3.38 ± 0.36, 4.31 ± 0.57 ve 2.98 ± 0.15 µmol Troloks eşdeğeri/mL olarak bulunmuştur. Antioksidan aktiviteleri, toplam fenolik madde içeriği ve ORAC bakımından ürünler arasında en düşük değerler sade süt örneklerinde tespit edilmiştir. Çikolatalı sütlerde toplam antioksidan aktivite değerleri en yüksek belirlenmiştir p

References

  • AOAC 1997. Method 945.46, 947.05, 986.33, 989.04, 990.19. Official methods of analysis of AOAC international (16th ed.). Gaithersburg, MD 20877-2417. USA.
  • Benzie, I.F.F., 2003. Evolution of dietary antioxidants. Physiology Part A, 136:113-126. Biochemistry and
  • Çakatay, U., Kayalı, R., 2006. Serbest radikal biyokimyasının Cerrahpaşa Tıp Dergisi 37: 162-167. süreçteki gelişimi.
  • Çavdar, C., Sifil, A., Çamsarı, T., 1997. Reaktif oksijen partikülleri ve antioksidan savunma. Türk Nefroloji Diyaliz ve Transplantasyon Dergisi 3-4: 92- 95.
  • Cervato, G., Cazzola, R. & Cestaro, B., 1999. Studies on the antioxidant activity of milk caseins. International Journal of Food Sciences and Nutrition, 50: 291-296.
  • Clausen, M.R., Skibsted, L.H., Stagsted, J. 2009. Characterization of major radical scavenger species in chromatography and functional assays. Journal of Agricultural and Food Chemistry 57(7): 2912-2919.
  • Dávalos, A., Bartolomé, B., Gómez-Cordovés, C., 2004. Extending applicability of the oxygen radical absorbance capacity (ORAC-Fluorescein) assay. Journal of Agricultural and Food Chemistry 52: 48- 54.
  • Gu, L., House, S. E., Wu, X., Ou, B., & Prior, R. L. 2006. Procyanidin and catechin contents and antioxidant capacity of cocoa and chocholate products. Journal of Agricultural and Food Chemistry 54: 4057-4061.
  • Hudson, BJF., 1990. Food Antioxidants. Elsevier Applied Science Publishers, New York. Elsevier, New York, pp. 253–307.
  • Jiménez, A.M., Murcia, M.A., Parras, P., Martínez- Tomé, M. 2008. On the importance of adequately choosing the ingredients of yoghurt and enriched milk for their antioxidant activity. International Journal of Food Science and Technology. 43(8): 1464-1473.
  • O'Connel, J.E., and Fox, P.F. 2001. Significance and application of phenolic compounds in the production and quality of milk and dairy products: a review. International Dairy Journal 11(3):103-120.
  • Ostdal, H., Daneshvar, B., Skibsted, L., 1996. Reduction of ferrylmyoglobin by b-lactoglobulin. Free Radical Research 24: 429-438.
  • Pihlanto, A., 2006. Antioxidative peptides derives from milk proteins. International Dairy Journal 16, 1306-1314.
  • Piljac-Žegarac, J., Valek, L., Martinez, S., Belščak, A., 2009. Fluctuations in the phenolic content and antioxidant capacity of dark fruit juices in refrigerated storage. Food Chemistry 113: 394-400.
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C., 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine 26: 1231-1237.
  • Saenz, A.T., Elisia, I., Innis, S.M., Friel, J. K., Kitts, D. D. 2009. Use of ORAC to assess antioxidant capacity of human milk. Journal of Food Composition and Anaysis 22 (7-8): 694-698.
  • SAS Institute Inc., Version 8, Cary, NC, USA, 1999.
  • Shahidi, F. 2000. Antioxidants in Food and Food Antioxidants, Nahrung, 44,158-163.
  • Singleton, V.L., Orthofer, R., Lamuela-Raventos, R.M. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent. Methods in Enzymology 299: 152-178.
  • Steinberg, F. M., Bearden, M. M., Keen, C. L., 2003. Cocoa and chocolate flavonoids: Implications for cardiovascular health. Journal of the American Dietetic Association 103(2): 215-223.
  • Tong, L.M., Sasaki, S., McClements, D.J., Decker, E.A., 2000. Mechanisms of the antioxidant activity of a high molecular weight fraction of whey. Journal of Agriculture and Food Chemistry 48: 1473-1478.
  • TSE (Türk Standartları Enstitüsü) 2001. Uzun Ömürlü Süt Standardı. T.S. 1192. Ankara.
  • TSE (Türk Standartları Enstitüsü) 2002. Aromalı Süt Standardı. T.S. 5004. Ankara.
  • Tunalıer, Z., Öztürk, N., Koşar, M., Başer, K H C., Duman, H., Kırımer, N., 2002. Bazı sideritis türlerinin antioksidan etki ve fenolik bileşikler yönünden Hammaddeleri Toplantısı, 130-138, Eskişehir.
  • Yanishlieva, N V., Pokomy, J., Gordon, M., 2001. Bitkisel İlaç Inhibiting Oxidation in Antioxidants in Food: Practical Applications., CRC press LLC and Woodhead Publishing Ltd, New York, USA, 288s.
  • Zulueta, A., Esteve, M. J., Frasquet, I., Frígola, A., 2007. Vitamin C, vitamin A, phenolic compounds and total antioxidant capacity of new fruit juice and skim milk mixture beverages marketed in Spain. Food Chemistry 103: 1365-1374.
There are 26 citations in total.

Details

Primary Language Turkish
Journal Section Research Article
Authors

Ceren Sönmez This is me

Güldem Ertaş This is me

Özge Duygu Okur This is me

Zeynep Güzel Seydim This is me

Publication Date February 1, 2010
Published in Issue Year 2010 Volume: 8 Issue: 1

Cite

APA Sönmez, C., Ertaş, G., Okur, Ö. D., Güzel Seydim, Z. (2010). UHT Sütlerin Bazı Kalite Kriterlerinin ve Antioksidan Aktivitelerinin Belirlenmesi. Akademik Gıda, 8(1), 13-16.
AMA Sönmez C, Ertaş G, Okur ÖD, Güzel Seydim Z. UHT Sütlerin Bazı Kalite Kriterlerinin ve Antioksidan Aktivitelerinin Belirlenmesi. Akademik Gıda. February 2010;8(1):13-16.
Chicago Sönmez, Ceren, Güldem Ertaş, Özge Duygu Okur, and Zeynep Güzel Seydim. “UHT Sütlerin Bazı Kalite Kriterlerinin Ve Antioksidan Aktivitelerinin Belirlenmesi”. Akademik Gıda 8, no. 1 (February 2010): 13-16.
EndNote Sönmez C, Ertaş G, Okur ÖD, Güzel Seydim Z (February 1, 2010) UHT Sütlerin Bazı Kalite Kriterlerinin ve Antioksidan Aktivitelerinin Belirlenmesi. Akademik Gıda 8 1 13–16.
IEEE C. Sönmez, G. Ertaş, Ö. D. Okur, and Z. Güzel Seydim, “UHT Sütlerin Bazı Kalite Kriterlerinin ve Antioksidan Aktivitelerinin Belirlenmesi”, Akademik Gıda, vol. 8, no. 1, pp. 13–16, 2010.
ISNAD Sönmez, Ceren et al. “UHT Sütlerin Bazı Kalite Kriterlerinin Ve Antioksidan Aktivitelerinin Belirlenmesi”. Akademik Gıda 8/1 (February 2010), 13-16.
JAMA Sönmez C, Ertaş G, Okur ÖD, Güzel Seydim Z. UHT Sütlerin Bazı Kalite Kriterlerinin ve Antioksidan Aktivitelerinin Belirlenmesi. Akademik Gıda. 2010;8:13–16.
MLA Sönmez, Ceren et al. “UHT Sütlerin Bazı Kalite Kriterlerinin Ve Antioksidan Aktivitelerinin Belirlenmesi”. Akademik Gıda, vol. 8, no. 1, 2010, pp. 13-16.
Vancouver Sönmez C, Ertaş G, Okur ÖD, Güzel Seydim Z. UHT Sütlerin Bazı Kalite Kriterlerinin ve Antioksidan Aktivitelerinin Belirlenmesi. Akademik Gıda. 2010;8(1):13-6.

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