Ultrason Destekli Ekstraksiyon Yöntemi ile Elde Edilen Violasinin Antibakteriyel ve Antikanser Aktiviteleri

Year 2020, Volume: 18 Issue: 3, 241 - 246, 29.10.2020

Çağdaş Deniz Periz Seyhan Ulusoy Gülgün Tınaz Turgut Şekerler

Abstract

Viyolasin, özellikle Gram-negatif bakteri Chromobacterium violaceum tarafından üretilen ve antibakteriyel, antifungal, antioksidan ve antikanser etkilere sahip çeşitli bakteri türleri tarafından üretilen doğal mor bir pigmenttir. Bu çalışmada, viyolasin, Ultrason destekli (UA) ve mikrodalga destekli (MA) ekstraksiyon prosedürleri ile C. violaceum suşundan metanol, etanol, aseton ve etil asetat kullanılarak ekstrakte edilmiştir. Elde edilen viyolasin miktarı, yüksek performanslı sıvı kromatografisi (HPLC) kullanılarak belirlenmiştir. Daha sonra bu ekstraktların antibakteriyel ve antikanser aktiviteleri incelenmiştir. Bu çalışmada, viyolasin ilk kez UA ve MA ekstraksiyon yöntemleri kullanılarak elde edilmiştir. Viyolasin ekstrelerinin Staphylococcus aureus, S. aureus (MRSA) ve Bacillus cereus'a karşı antibakteriyel aktiviteye sahip olduğu belirlenmiştir. Viyolasin ekstresi rahim ağzı kanseri hücreleri (HeLa) üzerinde güçlü anti-kanser etkisi göstermiştir. Sonuçta, viyolasini ekstrakte etmek için, UA ve MA ekstraksiyon yöntemleri bakteri kültürlerine başarıyla uygulanmıştır.

Keywords

Viyolasin, Ekstraksiyon, Antibakteriyel, Antikanser

Antibacterial and Anticancer Activities of Violacein Extracted Through Ultrasound-Assisted Extraction Method

Abstract

Violacein is a natural violet pigment produced by various bacterial species, especially by the Gram-negative bacteria Chromobacterium violaceum. Violacein has antibacterial, antifungal, antioxidant and anticancer activities and has various applications in food, cosmetic, textile and pharmaceutical industries. In this study, violacein was extracted from the Chromobacterium violaceum culture through ultrasonic-assisted (UA) and microwave-assisted (MA) extraction methods using methanol, ethanol, acetone, and ethyl acetate as solvent. Violacein was quantified using High Performance Liquid Chromatography (HPLC). Then, antibacterial and anticancer activities of these extracts were investigated. In this study, for the first time, the violacein extract was obtained by using UA and MA extraction methods. The highest violacein concentration could be achieved by UAE method with methanol. It was found out that violacein extracts had an antibacterial effect against Staphylococcus aureus, S. aureus (MRSA) and Bacillus cereus. The violacein extract had a strong anticancer effect on cervical cancer (HeLa) cells. Finally, the UA and MA extraction methods were successfully applied to bacterial cultures in order to extract the violacein.

Keywords

Violacein, Extraction, Antibacterial, Anticancer

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