Vitreoscilla Hemoglobini Eksprese Eden Escherichia coli Suşları ile Şeker Pancarı Melasından Biyoetanol Üretiminde Ölçek Büyütmenin Etkisi

Year 2020, Volume: 18 Issue: 3, 264 - 269, 29.10.2020

Taner Şar Meltem Yeşilçimen Akbaş

https://doi.org/10.24323/akademik-gida.818111

Abstract

Bu çalışmada, Vitreoscilla hemoglobini eksprese eden E.coli TS3 ve TS4 suşlarının şeker pancarı melası hidrolizatı ile hazırlanan besiyerleri (MB2-MB5) kullanılarak biyoetanol üretimleri incelenmiştir. Kullanılan farklı şeker konsantrasyonlu besiyerleri içerisinde en fazla etanol üretimi MB2 besiyeri ortamında (yaklaşık %4 şeker içeren) gerçekleştirilmiştir. MB2 besiyerinde, küçük ölçekten büyük ölçeğe doğru biyoetanol üretiminin %10-17 oranında arttığı saptanmıştır. En fazla biyoetanol üretim miktarları en büyük hacimdeki fermentasyon ortamında TS3 ve TS4 suşları ile sırasıyla %2.49 ve %2.62 (v/v) olarak belirlenmiştir. Yapılan bu çalışmada ölçek büyütmenin VHb ekspresyonu yapan bakterilerle şeker pancarı melasından etanol üretimini olumlu etkilediği belirlenmiştir.

Keywords

Melas hidrolizatı, Etanol, Bakteriyel hemoglobin, E. coli, Sukroz, Ölçek büyütme

Supporting Institution

Gebze Teknik Üniversitesi

Project Number

2013-A02, 2016-A-13 ve 2017-A102-19

Thanks

Bu çalışma, Gebze Teknik Üniversitesi (2013-A02, 2016-A-13 ve 2017-A102-19) tarafından desteklenmiştir.

Effect of Scaling up on Bioetanol Production from Sugar Beet Molasses by Vitreoscilla Hemoglobin Expressing Escherichia coli Strains

Abstract

In the present work, bioethanol production through VHb expressing Escherichia coli TS3 and TS4 strains from sugar-beet molasses hydrolysate containing media was investigated. The highest growth and ethanol production were obtained in MB2 (contains about 4% sugar) medium. In MB2 medium, bioethanol production was enhanced by E. coli TS3 and TS4 strains from small scale to big scale fermentation. The highest ethanol productions by TS3 and TS4 strains were determined as 2.49% and 2.62% (v/v) respectively, with the largest volume of fermentation medium. It was shown that scaling up process had positive effect on bioethanol production from sugar beet molasses through VHb expressing strains.

Keywords

Molasses hydrolysate, Ethanol, Bacterial hemoglobin, E. coli, Scale up process

Project Number

2013-A02, 2016-A-13 ve 2017-A102-19

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