The Effects of Ovarian Activators, Follicle Stimulating Hormone, and Activin A on Estradiol and Prostaglandin Secretion

Abstract

ABSTRACT Objective: The ovarian follicle culture technique is the in vitro development process of an ovulatory follicle with a developmentally competent oocyte, starting from the primordial follicle. The culture of the ovarian follicles aims to preserve fertility through proper isolation and storage of the ovarian cortex, especially before the cancer treatments. This study aimed to observe the effect of follicle-stimulating hormone (FSH) and/or activin A on estradiol (E2) and prostaglandin (PGE2) secretion in bovine ovarian cortical strip culture system. Methods: Ovarian cortical strips from heifers were cultured in the presence of rhFSH (50 ng/mL), rhAct A (100 ng/mL), or a combination of these factors for 6 days. The culture media were collected and stored. The levels of estradiol and prostaglandin were determined by Enzyme-Linked Immunosorbent Assay (ELISA). Results: In the group cultured in the presence of Act A (100 ng/mL), a noticeable increase was observed in both E2 and PGE2 values compared to the other groups. This increase was significant. There was also an insignificant increase in PGE2 values in the FSH group compared to the control and FSH + Act A groups. Conclusions: The results of this study reveal changes in E2 and PGE2 secretions in ovarian follicle culture medium supplemented with activin A or FSH. In particular, activin A alone was observed to be significantly more effective in these secretions.

Keywords

• Activin A
• FSH
• Estradiol
• Prostaglandin
• Follicle Culture
• Oocyte Maturation

Ovaryum Aktivatörlerinden Folikül Stimülan Hormon ve Aktivin A'nın Östradiyol ve Prostaglandin Sekresyonu Üzerindeki Etkileri

Öz

ÖZ Amaç: Ovaryan folikül kültürü tekniği, primordiyal folikülden başlayarak gelişimsel olarak yetkin bir oosit içeren ovulatuvar folikülün in vitro gelişim sürecidir. Ovaryan folikül kültürü, özellikle kanser tedavileri öncesinde ovaryan korteksin uygun şekilde izole edilmesi ve saklanması yoluyla fertiliteyi korumayı amaçlamaktadır. Bu çalışma, sığır ovaryum kortikal şerit kültür ortamına folikül stimülan hormon (FSH) ve/veya aktivin A ilavesinin östradiyol (E2) ve prostaglandin (PGE2) sekresyonuna etkisini gözlemlemeyi amaçlamıştır. Yöntem: Dişi sığırlardan alınan ovaryum kortikal şeritleri, rhFSH (50 ng/mL), rhAct A (100 ng/mL) veya bu faktörlerin bir kombinasyonu ile 6 gün boyunca kültüre edildi. Kültür ortamları toplanarak saklandı. Östradiyol ve prostaglandin seviyeleri, Enzim Bağlı İmmünosorbent Testi (ELISA) ile belirlendi. Bulgular: Act A (100 ng/mL) varlığında kültüre edilen grupta, diğer gruplara kıyasla hem E2 hem de PGE2 değerlerinde belirgin bir artış gözlenmiştir. Bu artış istatistiksel olarak anlamlıydı. FSH grubundaki PGE2 değerlerinde de kontrol ve FSH + Act A gruplarına kıyasla anlamsız bir artış gözlenmiştir. Sonuçlar: Bu çalışmanın sonuçları, aktivin A veya FSH ilave edilmiş ovaryum folikül kültür ortamında E2 ve PGE2 sekresyonunda değişiklikler olduğunu ortaya koymaktadır. Özellikle tek başına activin A’nın bu sekresyonlarda anlamlı şekilde daha etkili olduğu gözlenmiştir.

Anahtar Kelimeler

• Aktivin A
• FSH
• Folikül kültürü
• Östradiyol
• Prostaglandin
• Oosit maturasyonu

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