Cloning the homologue of gene encoding full-length CBF1 transcription factor from Olea europaea L. leaves

Year 2023, , 292 - 299, 03.05.2023

Munise Yurtsever Maide Şeker , Şenay Vural Korkut

https://doi.org/10.35414/akufemubid.1205241

Abstract

One of the most prevalent environmental stresses that affects plants physically and physiologically is
the low temperature. Since low temperature adversely affects plant productivity Researchers
investigated the molecular processes that regulate the effects of cold stress on plants and discovered
many cold responsive genes as well as the control mechanisms that regulate them. CBF/DREB (C repeat
binding factor/dehydration response element binding factor) transcription factors induces the
expression of corresponding genes in plants in response to abiotic stress conditions such as cold,
drought and salinity. These transcription factors contribute to plant resistance to stress by activating
genes in various pathways. Genes encoding CBF transcription factors were first discovered in
Arabidopsis thaliana (L.). Later, Gene homologs were isolated and cloned from a variety of plants. They
belong to the AP2/EREBP protein family. In the present study, the full-length cDNA encoding homologue
of the transcription factor CBF1 was cloned from Olea europaea L. cv. Gemlik leaves. A 748 bp long
cDNA encodes a hypothetical protein of 224 amino acids. BLAST analysis revealed that the CBF1
sequences of Gemlik cultivar and wild olive were nearly identical. A phylogenetic tree was built using
Olea europea L. cv. Gemlik CBF1 sequence.

Keywords

CBF/DREB, Olea europaea L, Gene Cloning, DNA Sequencing, Phylogenetic analysis

Supporting Institution

TÜBİTAK

Project Number

1919B011703461

Thanks

This work was supported by The Scientific and Technological Research Council of Turkey (TÜBİTAK), project no 1919B011703461.

Olea europaea L. yaprağından tam uzunlukta CBF1 transkripsiyon faktörünü kodlayan gen homoloğunun klonlanması

Abstract

Bitkileri fiziksel ve fizyolojik olarak etkileyen en yaygın çevresel streslerden biri düşük sıcaklıktır. Düşük
sıcaklık bitki verimliliğini olumsuz etkilediğinden, araştırmacılar soğuk stresinin bitkiler üzerindeki
etkilerini düzenleyen moleküler süreçleri araştırmışlar ve soğuğa duyarlı genlerle birlikte bunların
kontrol mekanizmalarını da ortaya çıkarmışlardır. CBF/DREB (C tekrar bağlama faktörü/dehidrasyon
yanıt elemanı bağlama faktörü) transkripsiyon faktörleri, bitkilerde soğuk, kuraklık ve tuzluluk gibi
abiyotik stres koşullarına yanıt olarak ifade edilen genlerin ekspresyonunu indüklemektedir. Bu
transkripsiyon faktörleri, çeşitli yolaklardaki genleri aktive ederek strese karşı bitki direncine katkıda
bulunurlar. CBF transkripsiyon faktörlerini kodlayan genler ilk olarak Arabidopsis thaliana'da (L.)
keşfedildi. Daha sonra çeşitli bitkilerden gen homologları klonlandı. CBF/DREB transkripsiyon faktörleri
AP2/EREBP protein ailesi içinde yer alırlar. Bu çalışmada, transkripsiyon faktörü CBF1'in homologunu
kodlayan tam uzunluktaki cDNA, Olea europaea L. cv. Gemlik yapraklarından klonlanmıştır. 748 bp
uzunluğunda cDNA, 224 amino asitlik varsayımsal bir proteini kodlar. BLAST analizi, Gemlik çeşidinin ve
yabani zeytinin CBF1 dizilerinin neredeyse aynı olduğunu ortaya çıkardı. Olea europea L. cv Gemlik CBF1
dizisi kullanılarak bir filogenetik ağaç oluşturuldu.

Keywords

CBF/DREB, Olea europaea L, Gen Klonlama, DNA Dizileme, Filogenetik Analiz

Project Number

1919B011703461

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