Kekik (Thymus Vulgaris L.) Özütü Kullanarak Biyosentezlenmiş Gümüş Nanopartiküllerin Antibakteriyel Etkisinin Araştırılması

Year 2023, , 1551 - 1560, 28.12.2023

Cansu Güneş , Ahmet Avcı

https://doi.org/10.35414/akufemubid.1289809

Abstract

Nano boyutlu metaller, bakterilere karşı direnç oluşturma noktasında etkili birer antibakteriyel ajan olarak bilinir. Bu ajanlar özellikle insanlık için ciddi bir tehdit oluşturan ilaca dirençli bakterilerle mücadele etmek için doğal kökenli alternatifler oluşturmaktadır. Gümüş nanopartiküllerin (AgNP'ler) iyi derecede antimikrobiyal aktiviteye sahip olduğu kanıtlanmıştır. AgNP'lerin biyosentezi diğer nanopartikül üretim yöntemlerine kıyasla daha güvenli, düşük maliyetli ve çevre dostu bir yöntem olarak kabul edilmektedir. Bu çalışmada, biyosentez yöntemi kullanılarak Thymus Vulgaris L. sulu özütüyle enkapsüle olmuş gümüş nanopartikül (eAgNP) üretimi başarıyla gerçekleştirilmiştir. AgNP’lere kekik bitkisinin antiseptik, antimikrobiyal ve antioksidatif özelliklerinin kazandırılması amaçlanmıştır. eAgNP üretimini sağlayacak optimum bitki özütü ve AgNO3 konsantrasyonu UV-vis spektrofotometre analizi ile belirlenerek bulunan oranlar doğrultusunda yeşil sentez gerçekleştirilmiştir. Sentezlenen eAgNP'ler UV-Vis spektral analizi, FT-IR, XRD analizi ve TEM görüntü analizi ile karakterize edilmiştir. eAgNP'lerin antimikrobiyal aktivitesi E. coli ve S. aureus bakterileri kullanılarak disk difüzyon metodu ile araştırılmıştır. TEM analizi ile biyosentezlenmiş eAgNP'lerin bitki özütü ile enkapsülasyonunun gerçekleştiği ve 6-70 nm boyut aralığında küresel bir şekle sahip olduğu bulunmuştur. eAgNP'leri çevreleyen bitki kaynaklı fitokimyasalların varlığı FT-IR ile, AgNP oluşumu ise XRD analizi ile kanıtlanmıştır. 5 mg/mL'lik bir konsantrasyonda disklere emdirilen eAgNP'ler, E. coli ve S.aureus bakterilerine karşı sırasıyla 20.5 mm ve 23.2 mm ortalama inhibisyon çapları ile antimikrobiyal aktivite göstermiştir. Sonuçlar, oldukça güçlü antimikrobiyal aktiviteye sahip, çevre dostu eAgNP'lerin Thymus Vulgaris L. özütü kullanılarak hazırlanabileceğini ve biyomalzeme alanında önemli bir potansiyel oluşturabileceğini ortaya koymuştur.

Keywords

Antibakteriyel aktivite, Biyosentez, Enkapsülasyon, Gümüş nanopartikül, Thymus vulgaris l.

Supporting Institution

Necmettin Erbakan Üniversitesi Bilimsel Araştırma Projeleri

Project Number

211319018

Thanks

Bu çalışma, Necmettin Erbakan Üniversitesi Bilimsel Araştırma Projeleri tarafından Yüksek Lisans Tez Projesi olarak desteklenen projenin bulgularının bir kısmını içermektedir (Proje No: 211319018).

Investigation of Antibacterial Effect of Biosynthesized Silver Nanoparticles Using Thyme (Thymus Vulgaris L.) Extract

Abstract

Nano-sized metals are known as effective antibacterial agents at the point of creating resistance against bacteria. These agents are alternatives of natural origin to struggle drug-resistant bacteria, which pose a serious threat to humanity. Silver nanoparticles (AgNPs) have been proven to have good antimicrobial activity. Biosynthesis of AgNPs is accepted as a safer, lower cost, and more environmentally friendly method compared to other nanoparticle production methods. In this study, silver nanoparticles (eAgNPs) encapsulated with Thymus Vulgaris L. aqueous extract were successfully produced using the biosynthesis method. It is aimed to bring in the antiseptic, antimicrobial, and antioxidative properties of the thyme plant to AgNPs. The optimum plant extract and AgNO3 concentration that is required for eAgNP production were determined by UV-vis spectrophotometer analysis and green synthesis was carried out in line with the ratios found. The synthesized eAgNPs were characterized by UV-Vis spectral analysis, FT-IR, XRD analysis, and TEM analysis. The antimicrobial activity of eAgNPs was investigated using a disc diffusion method against E. coli and S. aureus. It has been confirmed that the encapsulation of biosynthesized eAgNPs with the plant extract was successfully carried out by TEM analysis and spherical-shaped biosynthesized nanoparticles had a size range of 6-70 nm. The presence of plant-derived phytochemicals surrounding eAgNPs was confirmed by FT-IR and the formation of AgNP was analyzed by XRD spectrum. Biosynthesized eAgNPs adsorbed into discs at a concentration of 5 mg/mL showed antimicrobial activity against E. coli and S. aureus with mean inhibition diameters of 20.5 mm and 23.2 mm, respectively. The results introduced that eco-friendly eAgNPs with strong antimicrobial activity can be prepared using Thymus Vulgaris L. plant extract and may create a significant potential in the field of biomaterials.

Keywords

Antibacterial activity, Biosynthesis, Encapsulation, Silver nanoparticles, Thymus vulgaris l.

Project Number

211319018

References

• Al-Radadi, N. S. (2022). Laboratory scale medicinal plants mediated green synthesis of biocompatible nanomaterials and their versatile biomedical applications. Saudi Journal of Biological Sciences, 29(5), 3848-3870
• Alao, I. I., Oyekunle, I. P., Iwuozor, K. O., & Emenike, E. C. (2022). Green synthesis of copper nanoparticles and investigation of its anti-microbial properties. Advanced Journal of Chemistry-Section B, 4(1), 39-52.
• Alsammarraie, F. K., Wang, W., Zhou, P., Mustapha, A. ve Lin, M. (2018). Green synthesis of silver nanoparticles using turmeric extracts and investigation of their antibacterial activities. Colloids and Surfaces B: Biointerfaces, 171, 398-405.
• Asif, M., Yasmin, R., Asif, R., Ambreen, A., Mustafa, M. ve Umbreen, S. (2022). Green Synthesis of Silver Nanoparticles (AgNPs), Structural Characterization, and their Antibacterial Potential. Dose Response, 20(2), 15593258221088709.
• Aytac, Z., Ipek, S., Durgun, E., Tekinay, T. ve Uyar, T. J. F. c. (2017). Antibacterial electrospun zein nanofibrous web encapsulating thymol/cyclodextrin-inclusion complex for food packaging. Food chemistry, 233, 117-124.
• Balciunaitiene, A., Viskelis, P., Viskelis, J., Streimikyte, P., Liaudanskas, M., Bartkiene, E., . . . Lele, V. (2021). Green Synthesis of Silver Nanoparticles Using Extract of Artemisia absinthium L., Humulus lupulus L. and Thymus vulgaris L., Physico-Chemical Characterization, Antimicrobial and Antioxidant Activity. Processes, 9(8), 1304.
• Banerjee, P., Satapathy, M., Mukhopahayay, A., Das, P. J. B. ve Bioprocessing. (2014). Leaf extract mediated green synthesis of silver nanoparticles from widely available Indian plants: synthesis, characterization, antimicrobial property and toxicity analysis. Bioresources and Bioprocessing, 1, 1-10.
• Boskovic, M., Zdravkovic, N., Ivanovic, J., Janjic, J., Djordjevic, J., Starcevic, M. ve Baltic, M. Z. J. P. F. S. (2015). Antimicrobial activity of thyme (Tymus vulgaris) and oregano (Origanum vulgare) essential oils against some food-borne microorganisms. Procedia Food Science, 5, 18-21.
• Castillo-Henríquez, L., Alfaro-Aguilar, K., Ugalde-Álvarez, J., Vega-Fernández, L., Montes de Oca-Vásquez, G. ve Vega-Baudrit, J. R. J. N. (2020). Green synthesis of gold and silver nanoparticles from plant extracts and their possible applications as antimicrobial agents in the agricultural area. Nanomaterials, 10(9), 1763.
• Chakravarty, A., Ahmad, I., Singh, P., Ud Din Sheikh, M., Aalam, G., Sagadevan, S. ve Ikram, S. (2022). Green synthesis of silver nanoparticles using fruits extracts of Syzygium cumini and their bioactivity. Chemical Physics Letters, 795, 139493.
• De Melo, A. P. Z., Maciel, M. V. d. O. B., Sganzerla, W. G., da Rosa Almeida, A., de Armas, R. D., Machado, M. H., . . . Barreto, P. L. M. (2020). Antibacterial activity, morphology, and physicochemical stability of biosynthesized silver nanoparticles using thyme (Thymus vulgaris) essential oil. Materials Research Express,7(1), 015087.
• Gholami, M., Shahzamani, K., Marzban, A. ve Lashgarian, H. E. J. I. n. (2018). Evaluation of antimicrobial activity of synthesised silver nanoparticles using Thymus kotschyanus aqueous extract. IET nanobiotechnology, 12(8), 1114-1117.
• Güneş Çimen, C., Dündar, M. A., Demirel Kars, M. ve Avcı, A. (2022). Enhancement of PCL/PLA Electrospun Nanocomposite Fibers Comprising Silver Nanoparticles Encapsulated with Thymus Vulgaris L. Molecules for Antibacterial and Anticancer Activities. ACS Biomaterials Science & Engineering, 9, 3717-3732.
• Hashim, N., Paramasivam, M., Tan, J. S., Kernain, D., Hussin, M. H., Brosse, N., . . . Raja, P. B. (2020). Green mode synthesis of silver nanoparticles using Vitis vinifera’s tannin and screening its antimicrobial activity / apoptotic potential versus cancer cells. Materials Today Communications, 25, 101511.
• Khalil, M. A., El-Shanshoury, A. E.-R. R., Alghamdi, M. A., Alsalmi, F. A., Mohamed, S. F., Sun, J. ve Ali, S. S. (2022). Biosynthesis of Silver Nanoparticles by Marine Actinobacterium Nocardiopsis dassonvillei and Exploring Their Therapeutic Potentials. Frontiers in Microbiology, 12, 705673.
• Khurana, C., Vala, A. K., Andhariya, N., Pandey, O. ve Chudasama, B. J. J. o. B. M. R. P. A. (2014). Antibacterial activity of silver: the role of hydrodynamic particle size at nanoscale. Journal of Biomedical Materials Research Part A, 102(10), 3361-3368.
• Manikkannan, T., Umapathi, B., Behera, S., Panda, P. ve Mishra, B. (2013). Biosynthesis of silver nanoparticle from leaf extract of Desmodium gangeticum (L.) DC. and its biomedical potential. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 116, 424-427.
• Manivasagan, P., Venkatesan, J., Senthilkumar, K., Sivakumar, K. ve Kim, S.-K. (2013). Biosynthesis, antimicrobial and cytotoxic effect of silver nanoparticles using a novel Nocardiopsis sp. MBRC-1. BioMed research international, 2013.
• Manukumar, H., Yashwanth, B., Umesha, S. ve Rao, J. V. J. A. J. o. C. (2020). Biocidal mechanism of green synthesized thyme loaded silver nanoparticles (GTAgNPs) against immune evading tricky methicillin-resistant Staphylococcus aureus 090 (MRSA090) at a homeostatic environment. Arabian Journal of Chemistry, 13(1), 1179-1197.
• Nowak, A., Kalemba, D., Krala, L., Piotrowska, M. ve Czyzowska, A. (2012). The effects of thyme (Thymus vulgaris) and rosemary (Rosmarinus officinalis) essential oils on Brochothrix thermosphacta and on the shelf life of beef packaged in high-oxygen modified atmosphere. Food Microbiol, 32(1), 212-216.
• Nowak, A., Kalemba, D., Piotrowska, M. ve Czyowska, A. J. A. J. o. M. R. (2013). Effects of thyme (Thymus vulgaris L.) and rosemary (Rosmarinus officinalis L.) essential oils on growth of Brochothrix thermosphacta. Afr. J. Microbiol. Res, 7, 3396-3404.
• Pandiyan, I., Sri, S. D., Indiran, M. A., Rathinavelu, P. K., Prabakar, J. ve Rajeshkumar, S. (2022). Antioxidant, anti-inflammatory activity of Thymus vulgaris-mediated selenium nanoparticles: An in vitro study. J Conserv Dent, 25(3), 241-245.
• Patel, R. R., Singh, S. K. ve Singh, M. (2023). Green synthesis of silver nanoparticles: methods, biological applications, delivery and toxicity. Materials Advances, 4(8), 1831-1849.
• Polat, G., Erdal, Z. A. ve Kalay, Y. E. (2020). Design of novel non-equiatomic Cu-Ni-Al-Ti composite medium-entropy alloys. Journal of Materials Engineering and Performance, 29, 2898-2908.
• Rakaa, J. M. ve Obaid, A. S. J. I. J. o. P. (2020). Biosynthesis of silver nanoparticles using Thyme vulgaris leaves extract and its antibacterial activity. Iraqi Journal of Physics, 18(46), 1-12.
• Roy, P., Das, B., Mohanty, A. ve Mohapatra, S. J. A. N. (2017). Green synthesis of silver nanoparticles using Azadirachta indica leaf extract and its antimicrobial study. Applied Nanoscience, 7(8), 843-850.
• Singh, H., Du, J., Singh, P. ve Yi, T. H. (2018). Ecofriendly synthesis of silver and gold nanoparticles by Euphrasia officinalis leaf extract and its biomedical applications. Artificial cells, nanomedicine, and biotechnology,46(6), 1163-1170.
• Thakur, B. K., Kumar, A. ve Kumar, D. (2019). Green synthesis of titanium dioxide nanoparticles using Azadirachta indica leaf extract and evaluation of their antibacterial activity. South African Journal of Botany, 124, 223-227.
• Usmani, A., Mishra, A., Jafri, A., Arshad, M. ve Siddiqui, M. A. (2019). Green synthesis of silver nanocomposites of Nigella sativa seeds extract for hepatocellular carcinoma. Current Nanomaterials, 4(3), 191-200.
• Wasilewska, A., Klekotka, U., Zambrzycka, M., Zambrowski, G., Święcicka, I. ve Kalska-Szostko, B. (2023). Physico-chemical properties and antimicrobial activity of silver nanoparticles fabricated by green synthesis. Food Chemistry, 400, 133960.
• Yılmaz Öztürk, B., Yenice Gürsu, B. ve Dağ, İ. (2020). Antibiofilm and antimicrobial activities of green synthesized silver nanoparticles using marine red algae Gelidium corneum. Process Biochemistry, 89, 208-219.
• Zhang, D., Ma, X. L., Gu, Y., Huang, H. ve Zhang, G. W. (2020). Green Synthesis of Metallic Nanoparticles and Their Potential Applications to Treat Cancer. Front Chem, 8, 799.