Optimization of Bioprotein Production by Pleurotus ostreatus in Submerged Culture Conditions

Year 2013, , 35 - 42, 01.04.2013

Cem Özkan Mustafa Yamaç Zeki Yıldız

https://doi.org/10.5578/fmbd.5905

Cited By: 1

Abstract

In this study, the effects of environmental factors on mycelium development of Pleurotus ostreatus was investigated. Besides, optimum nutritional factors for bioprotein production by the isolate were selected by Plackett-Burman design. Temperature of 30 °C, pH 8 and 4% inoculant size were selected among 7 incubation temperature (15-45 °C), 9 pH values (4.0-8.0), and 9 inoculant size (1-16%) for maximum mycelial growth of the isolate. In the case of Pleurotus ostreatus, dog food, lactose, corn steep liquor, gibberellic acid, CaCl2 and KH2POsignificantly influenced the bioprotein production. © Afyon Kocatepe Üniversitesi

Keywords

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Pleurotus ostreatus Makrofungusu ile Derin Kültür Koşullarında Biyoprotein Üretiminin Optimizasyonu

Abstract

Bu çalışmada, Pleurotus ostreatus makrofungus izolatının misel gelişimi üzerine etki eden çevresel faktörler belirlenmiştir. Ayrıca izolatın biyoprotein üretimi için en uygun besinsel bileşenler PlackettBurman yöntemi ile seçilmiştir. Araştırma sonucunda 7 farklı inkübasyon sıcaklığı (15–45 °C), 9 farklı pH derecesi (4.0-8.0) ve 9 farklı inokülant miktarı (1-16 %) arasından en iyi misel gelişimi gözlenen sıcaklık 30 °C, pH 8 ve inokülant miktarı % 4 olarak belirlenmiştir Köpek yemi, laktoz, corn steep liquor, giberellik asit, CaCl2 ve KH2PO4 içerikli besiyerinin Pleurotus ostreatus izolatının biyoprotein üretimini önemli oranda arttırdığı belirlenmiştir

Keywords

Biyoprotein, Derin kültür, Pleurotus ostreatus, PlackettBurman

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