Q Humması Hastalığının Antijenik Peptidinin Sentezi ve Karakterizasyonu

Year 2017, Volume: 17 Issue: 1, 312 - 317, 24.04.2017

Mesut Karahan

Abstract

Q humması gram negatif Coxiella burnetii bakterisinin sebep olduğu tüm Türkiye ve Dünya’da yaygın olarak görülen zoonotik bir hastalıktır ve Q humması hastalığı için aşı geliştirme çalışmaları devam etmektedir. Biyoteknolojik aşılardan peptid aşıları sahip olduğu avantajlar ile ön plana çıkmakta ve son yıllarda yaygın olarak çalışılmaktadır. Bu çalışmada Q humması hastalığına ait m1E41920 peptid epitopu (NH2- Ser - Leu - Thr - Trp - His - Lys - His - Glu - Leu - His - Arg - Lys –COOH) mikrodalga destekli katı faz peptid sentezi yöntemiyle sentezlenmiştir. Bu peptidin saflaştırılması ve karakterizayonu yüksek basınçlı sıvı kromatografisi ve kütle spektrometresi ile yapılmıştır. Bu çalışmadan elde edilen saf peptid polimerik adjuvanlara bağlanarak Q humması hastalığı için yeni nesil sentetik peptid aşısı geliştirilmesinde kullanılacaktır.

Antigenic Peptide Synthesis and Characterization

Keywords

Katı Fazda Peptid Sentezi, Q Humması, Sentetik Peptid Aşısı

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